We narrowed to 1,078 results for: CAN

...reproducibility in science. General Considerations The ELISA can be completed in a single day or broken up over several...best concentration for your assay. Washing steps can be done by hand but if available, use an automated... to add 200 µL wash buffer to each well. Washing can be done by hand but if available, use an automated...

... about Henrietta Lacks - the African American woman whose cervical cancer cells became a mainstain of ...

...glycerol stock. Pro-Tip If you use a wire loop you can sterilize it by passing it through a flame, just ...angle, the way you would hold a pencil, so that you can make a broad stroke. Only touch the surface of the...single colonies. Once you have single colonies, you can proceed to Recovering Plasmid DNA or use the individual...

...an organization that raises money for childhood cancer through the sale of art. About two years ago, I...Northeast Human Resources Association, Association of American Medical Colleges and the Society for Human Resource...501c3 public charity raising funds for childhood cancer through the sale of art. Past volunteer appointments...

...Introduction This protocol can be used to purify AAV of any serotype. In medicine, iodixanol can be used as an intravenous...rotor at 10 °C. Pro-Tip If you need more time, you can alternatively centrifuge for 2 h at 200,000 x g at...used to collect fractions from the gradient. You can collect fewer fractions once you have a good idea... 3 mL collected corresponds to the 60% phase and can be discarded. The fractions obtained from the 40%... to collect the solution, or you will lose a significant amount of your virus. When the first fraction...

...A wide variety of protocols from Addgene that can be used for basic molecular biology, plasmid cloning...page you'll find practical lab protocols that you can use for a wide range of applications, with videos...for select protocols in the right-hand column. You can find even more video content on the Addgene Video...

... the recombinant antibody can be used as-is or the recombinant antibody can be purified from the supernatant...transfection and expression, the recombinant antibody can be purified for use in a variety of applications....supplement the flask with 4% BCD Feed. Pro-Tip The feed can be repeated up to 4 times for a total of 16% of the...Pro-Tip The optimal ratio of DNA:PEI may vary significantly and should be empirically determined for your...

...expedite research. We recognize, however, that we can’t do it all ourselves. Therefore, in this blog post... write for the Addgene blog simply email us. We can’t guarantee that all those who sign up will be able...

...expression of shRNAs. pLKO.1 can be introduced into cells via direct transfection, or can be converted into lentiviral...plasmid for producing viral particles. Note: pLKO.1 can also be used with packaging plasmid pCMV-dR8.2 dvpr...Biomedical Research hosts an siRNA Selection Program that can be accessed after a free registration. Guidelines...pLKO.1 vector was created. Additional design rules can be found at the Broad Institute's TRC shRNA Design...that two different shRNAs that target the same gene can produce the same phenotype will alleviate concerns... on 0.8% low melting point agarose gel until you can distinctly see 2 bands, one 7kb and one 1.9kb. Cut...is for transfection in a 6 cm plate. The protocol can be scaled to produce different amounts of virus as...

... at Addgene. Stolzenburg S, Beltran AS, Swift-Scanlan T, Rivenbark AG, Rashwan R, Blancafort P (2015)... and targeted de novo DNA methylation in breast cancer. Oncogene 34:5427–5435 . https://doi.org/10.1038...

...specifically on selection with carbenicillin; however it can be adapted for any antibiotic through the use of ...liquid is mostly absorbed. The spreading of cells can be done in the same way as the antibiotic, using ...

...polymerase/exonuclease functions, T4 DNA polymerase can create overhangs of varying length (typically 10-...specified distance from their recognition sequence. This can create multiple distinct overhangs with a single ...

...alone, we had 12,427 plasmids deposited with us. We cannot say thank you enough - but this week we’re certainly...

...confident in your application and protocols, but can’t quite seem to find an antibody that works, we highly...

...excited to see your thoughts. Our mascot Blugene can’t wait either. Acknowledgements We would like to ...

... Addgenie, from our co-ops to our directors, we can't celebrate Addgene without recognizing our incredible...

...time of year for those of us at Addgene! And we can’t think of any better way to start off our holiday...

...That’s one sticky situation our customer service can’t get you out of.           View this post on ...

...awarded plasmids from Addgene as a successful applicant to the Addgene, Seeding Labs Plasmid Grant. For...

...touch? The whole trouble with houseplants is they can’t bark, meow, or cry when they need something. But...