We narrowed to 1,082 results for: CAN

...you can easily scan the plate for individual cells, which should be on the same focal plane. Scan the ...the microscope later when scanning the plate for individual cells, which can be difficult to find. Leave...calcium or magnesium, Corning 21-040-CV (cations can affect the attachment of adherent cells) Trypsin ...this protocol. Conditioned medium (details below) can be used to enhance the growth of cells in individual...protocol has been optimized for adherent cells but can be modified for suspension cells. The number of monoclonal... that the cells have detached from the plate and can be isolated. When trypsinized, detached cells will...cells for 13 days. Note that growth rate differences can be inferred by the differences in colony size. Figure...

...primer, one can include more than one nucleotide at that position termed a mixed site. One can also use ...completely to the template DNA strand so elongation can proceed. Usually a guanine or cytosine is used at...

...wearing open-toed shoes, cloth coverings or “booties” can be used to protect your feet. Pro-Tip If they are...Eye protection such as goggles and/or face shields can be used as needed. For BSL-2 work always wear glasses...administrators, or anyone else associated with Addgene, can be held responsible for your use of the information...be snug to your hand, but not so tight that you cannot perform your duties. Be sure to try out different...

...attached to the backbone and the complete plasmid can be transformed into bacterial cells for propagation...Whenever you need to set up ligations in the future you can thaw a new tube that you know has only been thawed...ligation of annealed oligos) the efficiency of ligation can be improved by incubation at 37°C. Proceed with bacterial...insert to vector ratio is usually sufficient, you can optimize the amount of insert and vector to improve...If the DNA concentrations are low such that you cannot get all 100ng of DNA, buffer and ligase into a ...

...reproducibility in science. General Considerations The ELISA can be completed in a single day or broken up over several...best concentration for your assay. Washing steps can be done by hand but if available, use an automated... to add 200 µL wash buffer to each well. Washing can be done by hand but if available, use an automated...

...glycerol stock. Pro-Tip If you use a wire loop you can sterilize it by passing it through a flame, just ...angle, the way you would hold a pencil, so that you can make a broad stroke. Only touch the surface of the...single colonies. Once you have single colonies, you can proceed to Recovering Plasmid DNA or use the individual...

... about Henrietta Lacks - the African American woman whose cervical cancer cells became a mainstain of ...

...an organization that raises money for childhood cancer through the sale of art. About two years ago, I...Northeast Human Resources Association, Association of American Medical Colleges and the Society for Human Resource...501c3 public charity raising funds for childhood cancer through the sale of art. Past volunteer appointments...

...Introduction This protocol can be used to purify AAV of any serotype. In medicine, iodixanol can be used as an intravenous...rotor at 10 °C. Pro-Tip If you need more time, you can alternatively centrifuge for 2 h at 200,000 x g at...used to collect fractions from the gradient. You can collect fewer fractions once you have a good idea... 3 mL collected corresponds to the 60% phase and can be discarded. The fractions obtained from the 40%... to collect the solution, or you will lose a significant amount of your virus. When the first fraction...

...A wide variety of protocols from Addgene that can be used for basic molecular biology, plasmid cloning...page you'll find practical lab protocols that you can use for a wide range of applications, with videos...for select protocols in the right-hand column. You can find even more video content on the Addgene Video...

... the recombinant antibody can be used as-is or the recombinant antibody can be purified from the supernatant...transfection and expression, the recombinant antibody can be purified for use in a variety of applications....supplement the flask with 4% BCD Feed. Pro-Tip The feed can be repeated up to 4 times for a total of 16% of the...Pro-Tip The optimal ratio of DNA:PEI may vary significantly and should be empirically determined for your...

...expedite research. We recognize, however, that we can’t do it all ourselves. Therefore, in this blog post... write for the Addgene blog simply email us. We can’t guarantee that all those who sign up will be able...

...expression of shRNAs. pLKO.1 can be introduced into cells via direct transfection, or can be converted into lentiviral...plasmid for producing viral particles. Note: pLKO.1 can also be used with packaging plasmid pCMV-dR8.2 dvpr...Biomedical Research hosts an siRNA Selection Program that can be accessed after a free registration. Guidelines...pLKO.1 vector was created. Additional design rules can be found at the Broad Institute's TRC shRNA Design...that two different shRNAs that target the same gene can produce the same phenotype will alleviate concerns... on 0.8% low melting point agarose gel until you can distinctly see 2 bands, one 7kb and one 1.9kb. Cut...is for transfection in a 6 cm plate. The protocol can be scaled to produce different amounts of virus as...

... at Addgene. Stolzenburg S, Beltran AS, Swift-Scanlan T, Rivenbark AG, Rashwan R, Blancafort P (2015)... and targeted de novo DNA methylation in breast cancer. Oncogene 34:5427–5435 . https://doi.org/10.1038...

...specifically on selection with carbenicillin; however it can be adapted for any antibiotic through the use of ...liquid is mostly absorbed. The spreading of cells can be done in the same way as the antibiotic, using ...

...polymerase/exonuclease functions, T4 DNA polymerase can create overhangs of varying length (typically 10-...specified distance from their recognition sequence. This can create multiple distinct overhangs with a single ...

...alone, we had 12,427 plasmids deposited with us. We cannot say thank you enough - but this week we’re certainly...

...confident in your application and protocols, but can’t quite seem to find an antibody that works, we highly...

...excited to see your thoughts. Our mascot Blugene can’t wait either. Acknowledgements We would like to ...

... Addgenie, from our co-ops to our directors, we can't celebrate Addgene without recognizing our incredible...