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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 1,053 results for: FER

Showing: 1041 - 1053 of 1053 results

  1. Pouring LB Agar Plates

    Type
    Protocol
    ... one or more antibiotic resistance genes, which confer resistance to a specific antibiotic to the bacteria... with resistance to that antibiotic - usually conferred by a plasmid carrying the antibiotic resistance...x (0.220 L) = 8.14 g pre-mixed LB-agar powder. Transfer the LB-agar powder you’ve measured out into an...molten agar from boiling over in the autoclave. Transfer the sterile water (in our case 220 mL) to the ...

  2. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ...backbone (See Addgene's Sequencing Primers for reference). Many sequencing cores have a list of common ...recommends using Addgene's sequencing results as a reference for primer design. A good sequencing reaction ...reaction with high background: If you obtain a different sequence than expected and wish to contact Addgene...

  4. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ... select transformed cells containing plasmids differing in their resistance genes, as one does not need...Sample Data Selection of E.coli on LB-agar using different concentrations of carbenicillin plated over-agar...

  5. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ... and this is used to determine how much of each buffer to add during the DNA isolation step. Finally, ...want to load 1.0 μg in 10μL, make 1.1μg in 11μL. Reference Page Top Index...

  6. Water Bath Protocol

    Type
    Protocol
    ... biology, and thaw cell lines. There are many different types of water baths that exist as scientists ...scientists work with tubes and bottles of different sizes in the lab. Some water baths contain individual slots...

  7. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ... of the four levels is to distinguish between different types of hazards and to provide protective measures...measures for each level, as each level has different safety requirements. BSL-1 is designated for those...

  8. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...restriction enzymes that can both function in the same buffer, as this will save time later. In our example, ...important that you sequence the final product. Reference Page Top Index...

  12. Fluorescence Titering Assay

    Type
    Protocol
    ...5 mL of 100X glutaGRO. Store at 4 °C. Pro-Tip Different brands and lots of FBS can promote or inhibit ...
Showing: 1041 - 1053 of 1053 results