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We narrowed to 969 results for: ARC

Showing: 571 - 580 of 969 results
  1. CRISPR Plasmids - dCas9-FokI

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    Collection
    ... off-target effects of CRISPR. Browse, sort, or search the tables below for CRISPR-FokI plasmids. Plasmids...
  2. Plasmids 101: Shuttle Vectors

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    Blog Post
    ...different kingdoms of life. That’s convenient for researchers who want to do experiments in complex eukaryotic...course, that includes you. Besides the risk to the researcher, Zeocin has been shown to cause mutations even...experiments. Reporters Finally, in some cases a researcher may choose not impose selection on cells and ...
  3. Plasmids 101: Codon usage bias

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    Blog Post
    ...useful for organisms, they can be problematic for researchers trying to express proteins in heterologous hosts... cost DNA synthesis, one of the primary ways researchers solve the problem of codon choice is to resynthesize...of these factors on their own. Luckily, many researchers have created codon optimization algorithms and...
  4. To Codon Optimize or Not: That is the Question

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    Blog Post
    ...amino acid across an entire polypeptide. Many researchers have created codon-optimization algorithms/resources...they can effectively inhibit translation. Genome Research 23:604–615 . https://doi.org/10.1101/gr.139758.112...databases: status for the year 2000. Nucleic Acids Research 28:292–292 . https://doi.org/10.1093/nar/28.1.292...
  5. Viral Production at Addgene

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    Blog Post
    ...of the transfer plasmid Addgene uses a rigorous barcode matching system that follows the sample from the...purification kits.     Figure 4: Schematic of the barcode matching system and PCR-based confirmation of transfer... plasmids used in Addgene’s viral production. Barcoding allows our DNA and virus to be monitored throughout...
  6. Important Considerations in Optogenetics Behavioral Experiments

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    Blog Post
    ...yet sometimes overlooked, aspect of scientific research is that the field benefits from collaboration ...neuroscience field is a great example of this; research benefits when the advances of some are shared ...help illuminate (is that pun worn out yet?) your research. Derek P. Simon received his PhD in Cellular ...
  7. Plasmids 101: The Promoter Region – Let's Go!

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    Blog Post
    ...not a big issue for cells, but it may confound research results or even kill your cells if your gene of...right tissue-specific promoters as described by researchers at the NIH here.  Note: A. Max Juchheim contributed...Resources on Addgene.org Find Plasmids for Your Research     ...
  8. CRISPR Plasmids - Plants

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    Collection
    ...Addgene's Empty gRNA Expression Vectors page and search the table for "Plant". Activate ID Plasmid Gene...
Showing: 571 - 580 of 969 results