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We narrowed to 772 results for: LIS

Showing: 101 - 120 of 772 results

  1. Zhang Lab CRISPR Page

    Type
    Collection
    ...DA, Weinstein JA, Ran FA, Konermann S, Agarwala V, Li Y, Fine EJ, Wu X, Shalem O, Cradick TJ, Marraffini...CRISPR-Cas9. Swiech L, Heidenreich 1, Banerjee A, Habib N, Li Y, Trombetta J, Sur M, Zhang F. Nat Biotechnol . ... of Cas9 in vivo and ex vivo , the Zhang Lab established Cre-dependent and constitutively expressing Cas9...

  3. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...PMID: 25514926. PubMed Central PMCID: PMC4383998. 3. Li, Tong, et al. "Cysteinyl‐tRNA formation: the last...difficulties with kinase specificity in vitro. The establishment of a system in which purified, recombinant protein...interested in the intersection of cell signaling, metabolism, and the mighty mitochondrion. You can follow...

  4. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...common diseases. References Zhang Y, Yin C, Zhang T, Li F, Yang W, Kaminski R, Fagan PR, Putatunda R, Young...viral reservoirs, and CRISPR may be the way to accomplish this tough job! Kamel Khalili’s lab at Temple...infected cells, an idea that became much more realistic with the advent of CRISPR. Using CRISPR/Cas9 SAM...enough T-cells could be reached to ablate a well-established viral reservoir. A second challenge for CRISPR...developing resistance. Shortly after Kaminski et al. published their results, Wang et al. showed that HIV-1 could...

  6. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...22205518. PubMed Central PMCID: PMC3574576. Lai, Sen-Li & Tzumin Lee. “Genetic mosaic with dual binary transcription...multiple genes at the same time.  The 3 binary systems listed above are orthogonal, meaning that they do not ...doi.org/10.1038/s41598-022-07852-7 De Boer, Herman A., Lisa K. Comstock & Mark Vasser. “The tac promoter: a ...

  7. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ... . https://doi.org/10.1038/mt.2009.280 Hirsch ML, Li C, Bellon I, Yin C, Chavala S, Pryadkina M, Richard...Iodice C, Polishchuk EV, Sommella A, Colecchi L, Rossi S, Simonelli F, Giunti M, Bacci ML, Polishchuk RS, Auricchio...Marrocco E, Rossi S, Giunti M, Palfi A, Farrar GJ, Polishchuk R, Auricchio A (2013) Effective delivery of large...

  8. Antibodies 101: Validation

    Type
    Blog Post
    ...Cordell JL, Cragg MS, Šerbec VČ, Jones M, Lisnic VJ, Kremer L, Li D, Koch-Nolte F, Pascual N, Rodríguez-Barbosa...unless you share the results with others. If you publish data generated using your antibody, it’s best scientific...

  9. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    .... Biol., Apr. 2016. PubMed PMID: 27075976. 4.  X. Li, E. R. Burnight, A. L. Cooney, N. Malani, T. Brady...enhanced random integration efficiencies that are accomplished with transfections and are therefore virus-free...enhance gene editing? Gene editing can now be accomplished in a more cost-effective and efficient manner...team.     Mathew Goodwin is a Technical Sales Specialist at Transposagen. As a gene editing enthusiast...Singh, V. V Adjan Steffey, T. Yeshi, and D. W. Allison, “Gene Editing in Human Pluripotent Stem Cells:... Perry, V. V. A. Steffey, K. Miller, and D. W. Allison, “Decoding the Epigenetic Heterogeneity of Human...

  10. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...science.aae0169 2. Long C, Amoasii L, Mireault AA, McAnally JR, Li H, Sanchez-Ortiz E, Bhattacharyya S, Shelton JM, ... treat disease postnatally. Now, three papers published concurrently in Science have shown CRISPR can ...

  11. Plan Your Experiment

    Type
    Collection
    ...4939-1862-1_10 PMID: 25408407 Dixit, A., Parnas, O., Li, B., Chen, J., Fulco, C. P., Jerby-Arnon, L., Marjanovic...j.cell.2014.09.029 PMID: 25307932 Glass, Z., Lee, M., Li, Y., & Xu, Q. (2018). Engineering the delivery system...exon coding for an essential protein domain could abolish protein activity and essentially function as a ...with identical genomes. If your end goal is to establish an edited cell line, for example, you will likely...

  12. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ... N., Ma, W., Liu, X., Cai, Y., Kou, J., Wang, J., Li, L., Peng, S., Xu, Z., Zhang, W., Qiu, Y., Wu, Y....the first paper identifying a binding receptor published this year (Dhungel et al., 2025)! It can transduce...contributing their expertise! This post was originally published in January 2025 and updated in August 2025. References...

  15. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...plasmids from this paper at Addgene. 6. Xing, Hui-Li, et al. “A CRISPR/Cas9 toolkit for multiplex genome...Originally published Jan 28, 2016 and last updated Sep 10, 2020 by Jennifer Tsang. CRISPR makes it easy...

  16. CRISPR References and Information

    Type
    Collection
    ...Xiaole Shirley Liu Lab . For more information, see Li et al. (2014) (Link opens in a new window) . protoSpaceJAM...Browse Addgene’s curated list of CRISPR resources and references, including gRNA design tools, CRISPR...Addgene CRISPR blogs CRISPR tips, FAQs, and news published on the Addgene blog. CRISPR Pooled Libraries Addgene's...experiments. Developed by the Hyongbum Kim Lab . Green Listed - a CRISPR Screen Tool (Link opens in a new window... custom CRISPR screens targeting a long or short list of genes provided by the user. Developed by the ...meningitidis Cas9, or Cas12a from your input sequence or list of sequences. Developed by the George Church Lab...activation). Genome support for bacteria ( E. coli, B. subtilis ), yeast ( S. cerevisiae ), worm ( C. elegans ...

  18. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...PDB ID 7S4X). The end of the DNA:RNA heteroduplex lies between the RuvC (orange) and HNH (red) domains,... will destabilize the nascent heteroduplex and abolish cleavage (Pattanayak et al., 2013). As mentioned...

  19. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...Wilson, Ning Sun, Deepak M. Gupta, Mei Huang, Zongjin Li, Nicholas J. Panetta, Zhi Ying Chen, Robert C. Robbins...reported in González et al. A percentage is not listed because many variables (cell type, criteria used...Check out the links at the end of the post for a list of Tet-inducible reprogramming vectors available...

  20. Deisseroth INTRSECT Collection

    Type
    Collection
    ...Zhao F, Luo MH, Gong L, He M, Zhou P, Paninski L, Li B. 2017. The central amygdala controls learning in...
Showing: 101 - 120 of 772 results