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  1. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...HiFi so accurate (apart from the high-fidelity polymerase) is the ability to remove, reset and ligate mismatched...primer design based on fragment sequences and the polymerase used in the amplification. This tool allows instant...

  2. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...and Oskar protein were detected at the posterior pole. Oskar protein levels were negatively correlated... translated, the SunTag sites on the nascent polypeptide recruit scFV-GFP, generating green puncta that...

  3. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ..., NIH3T3, CHO, 293HEK, Jurkat T cells Blocks polypeptide synthesis at 80S; inhibits chain elongation 100...hygB HeLa, NIH3T3, CHO, Jurkat T cells Blocks polypeptide synthesis at 80S; inhibits chain elongation. ...

  4. Antibodies 101: ChIP

    Type
    Blog Post
    ...incubate the sample with your antibody.  Both polyclonal and monoclonal antibodies can work well in ChIP...Antibodies 101: Immunoprecipitation  Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction to Antibodies...

  5. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...Cassettes transfection gRNAs expressed from a polycistronic transcript  Requires co-transfection of Cas9...Cassettes transfection gRNAs expressed from a polycistronic transcript Requires co-transfection of Cas9  ...

  7. The time and cost required to make a plasmid

    Type
    Blog Post
    ...plasmid? Figure 2: Survey results from a Twitter poll run in 2023, n=194.   After the great Plasmid...much harder to calculate, in both our 2018 and 2023 poll, most people said it took them more than 22 hours...

  8. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...shoulder for the picture. It tends to look like a police photo if you look straight at the camera. Pro tip...organizational work. LinkedIn is not the platform for politics, family updates, or click bait of any kind. For...

  9. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...professor of economics at the Andrew Young School of Policy Studies at Georgia State University, is an expert... in the intersection between science and public policy. Follow David on Twitter @driglar.     References...

  10. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...and Bester 2021). For example, inserting a polyadenylation signal downstream of the transcription start...fragment. (D) Knock-in: insertion of a synthetic polyadenylation (spA) signal. Figure and caption adapted from...

  11. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...such as the serotonin 3 receptor, will promote depolarization in neurons. Alternatively, combining with the...such as the glycine receptor, will promote hyperpolarization. PSAM ligands The advantage of PSAMs lies ...

  13. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ...In addition the transfection method (i.e. polyethyleneimine, calcium phosphate or liposomal methods), ...centrifugation on a sucrose gradient, spin columns, and polyethylene glycol (PEG) precipitation. All of these methods...

  14. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...). mAbs and rAbs have higher homogeneity than polyclonal antibodies because of their immunoglobin component...circumvents the batch-to-batch variability found in polyclonal antibodies, creating a potentially unlimited ...

  15. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...can influence the transcription of genes by RNA polymerase II, or by the addition of methyl- groups to the...9 methylation, while the K27 reporter uses the polycomb chromodomain to recognise lysine 27 methylation...

  16. TALEN Plasmids and Kits

    Type
    Collection
    ...specificity is determined by customizable arrays of polymorphic amino acid repeats in the TAL effectors. View...

  17. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...conference If the conference has a social media policy, this should be on their website or the program...program booklet. Make sure you’re familiar with the policies before posting something on social media. If someone...

  18. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ... myidp.sciencecareers.org mentioned museums. Indianapolis is home to one of "The world’s biggest and best...children’s museum", The Children's Museum of Indianapolis. I drove passed these dinosaurs every day. They...

  19. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...single mRNA. IRES was originally discovered in poliovirus RNA, where it promotes translation of the viral...eukaryotic mRNA directed by a sequence derived from poliovirus RNA. Pelletier et al (Nature. 1988 Jul 28;334...

  20. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...While monoclonal and polyclonal antibodies are readily available from several sources, fewer sources ...Antibodies Plasmid-based Recombinant Antibodies Polyclonal Antibodies Resources on addgene.org Addgene's...

  21. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...allows for the following to occur: A) Multiple polyclonal secondary IgGs can bind to different epitopes... Reagent Pros Cons Antibodies Bivalent Polyclonal Can be labeled with multiple dyes Commercially...

  22. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...CREATE cap plasmid, loxP sites flank the downstream polyA sequence. When viruses produced from this plasmid...into an animal with cell-type-specific Cre, the polyA sequence is inverted only in the Cre-expressing ...

  24. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...relevant parties, and the company at large, by polling and informal communications we made a decision ...scientists often prefer data. At Addgene we rely on polls and surveys. A mix of closed (multiple choice/binary...

  26. Tetracycline Inducible Expression

    Type
    Collection
    ...Expression of your gene of interest (MCS with a β-globin poly A) & EGFP from a bidirectional tet-responsive promoter...

  28. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...ensure this tRNA processing only occurs on the RNA polymerase III transcript, the sgRNAs were encoded on the...donor site; SAS, splice acceptor site; pA, polyadenylation signal. Image reused from Riedmayr et al. 2023...

  29. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...preparation for updating this post, I took an informal poll of Addgene hiring managers. All of them stated that...through in their resumes alone.  In my unofficial poll, hiring managers also said that they considered ...

  30. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...for your idea. Our product team has implemented a policy of never rejecting an idea simply because it's ...innovative group. Our entire company benefits from their policy! Perfectionism When we reach for perfection, our...
Showing: 151 - 180 of 362 results