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  1. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...CRISPR-Cas9 System. Appl Environ Microbiol 81:2506–2514 . https://doi.org/10.1128/aem.04023-14 Li Q, Sun B, Chen...Escherichia coli. Acta Biochimica et Biophysica Sinica. https://doi.org/10.1093/abbs/gmab036 Zhang Y, Sun X, Wang...CRISPR-Associated Transposases. ACS Synth Biol 9:1998–2008. https://doi.org/10.1021/acssynbio.0c00073   Additional...

  2. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    ...Journal of Neuroscience Methods, 172(2), 250–254. https://doi.org/10.1016/j.jneumeth.2008.05.003 Ghosh, ...blots. Expert Review of Proteomics, 11(5), 549–560. https://doi.org/10.1586/14789450.2014.939635 Gilda, J....blots. Analytical Biochemistry, 440(2), 186–188. https://doi.org/10.1016/j.ab.2013.05.027 Additional Resources...

  3. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...Molecular Biology. Springer New York, pp 299–311. https://doi.org/10.1007/978-1-4939-8935-5_26 Stuurman ...Introduction to Fluorescence Microscopy. iBiology. https://www.ibiology.org/talks/fluorescence-microscopy...validation of antibodies. Nat Methods 13:823–827. https://doi.org/10.1038/nmeth.3995 Additional Resources...

  4. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ... Tet-On and Tet-Off systems: Second generation. https://www.takarabio.com/products/gene-function/tet-inducible-expression-systems...in mammalian cells. BMC Biotechnol 6, 43 (2006). https://doi.org/10.1186/1472-6750-6-43Kaczmarczyk A, Vorholt...overexpression transgenes. Sci Rep 12, 3835 (2022). https://doi.org/10.1038/s41598-022-07852-7 De Boer, Herman...

  5. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...Mammalian Cell Culture. Anal Chem 84:4227–4232 . https://doi.org/10.1021/ac2033112 Ligasová A, Vydržalová...Mycoplasmas Using Fluorescence Microscopy. Cells 8:1510 . https://doi.org/10.3390/cells8121510 Sung H, Kang SH, ...Cell Lines. In Vitro Cell Dev Biol Anim 38:86 . https://doi.org/10.1290/1071-2690(2002)038<0086:caeomi...

  6. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    .... Review of Scientific Instruments 43:404–409 . https://doi.org/10.1063/1.1685647 McKinnon KM (2018) Flow...Overview. Current Protocols in Immunology 120: . https://doi.org/10.1002/cpim.40 Picot J, Guerin CL, Le...recent instrumentation. Cytotechnology 64:109–130 . https://doi.org/10.1007/s10616-011-9415-0 Additional resources...

  7. Lentiviral Vector Guide

    Type
    Guide
    ...Pseudotyping. Current Gene Therapy , 5 (4), 387–398. https://doi.org/10.2174/1566523054546224 PMID: 16101513...System. Journal of Virology , 72 (11), 8463–8471. https://doi.org/10.1128/jvi.72.11.8463-8471.1998 PMID:...of lentiviral vectors. Viruses , 3 (2), 132–159. https://doi.org/10.3390/v3020132 PMID: 22049307 Ghaleh.... Biomedicine & Pharmacotherapy , 128 , 110276. https://doi.org/10.1016/j.biopha.2020.110276 PMID: 32502836... Methods & Clinical Development , 21 , 451–465. https://doi.org/10.1016/j.omtm.2021.03.018 PMID: 33981779... Methods & Clinical Development , 20 , 792–800. https://doi.org/10.1016/j.omtm.2020.10.012 PMID: 33768124...Integration Targeting. PLoS ONE , 2 (12), e1340. https://doi.org/10.1371/journal.pone.0001340 PMID: 18092005...

  9. Adenovirus Guide

    Type
    Guide
    ...strategies . Current Gene Therapy, 11 (4), 307–320. https://doi.org/10.2174/156652311796150372 (Link opens...Signal Transduction and Targeted Therapy, 6 (1), 53. https://doi.org/10.1038/s41392-021-00487-6 (Link opens...virus vaccine . Scientific Reports, 10 (1), 3613. https://doi.org/10.1038/s41598-020-60238-5 (Link opens...Vaccines & Immunotherapeutics, 13 (12), 3020–3032. https://doi.org/10.1080/21645515.2017.1383575 (Link opens...challenge . Journal of Virology, 85 (9), 4222–4233. https://doi.org/10.1128/JVI.02407-10 (Link opens in a ... Expert Review of Vaccines, 14 (10), 1347–1357. https://doi.org/10.1586/14760584.2015.1077122 (Link opens...the United States of America, 95 (5), 2509–2514. https://doi.org/10.1073/pnas.95.5.2509 (Link opens in ...

  10. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    ... #WinterWonderlab @Addgene Make your own here!: https://t.co/AnltWfKuQg pic.twitter.com/OOrwo9feyJ — BTC...Remind them you care!#thanks #health #mentalhealth https://t.co/Td2M6AOpuW pic.twitter.com/ofLI4eD8Zs — Microbigals...

  11. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...nervous system . Molecular Therapy, 10 (2), 302–317. https://doi.org/10.1016/j.ymthe.2004.05.024 (Link opens...systems . Nature Neuroscience, 20 (8), 1172–1179. https://doi.org/10.1038/nn.4593 (Link opens in a new window...comparison . Molecular Therapy, 4 (4), 383–391. https://doi.org/10.1006/mthe.2001.0456 (Link opens in ...therapy . Current Gene Therapy, 3 (4), 281–304. https://doi.org/10.2174/1566523034578285 (Link opens in...production . Journal of Virology, 77 (8), 4881–4887. https://doi.org/10.1128/jvi.77.8.4881-4887.2003 (Link ...transfer . Pharmaceutical Research, 25 (3), 489–499. https://doi.org/10.1007/s11095-007-9431-0 (Link opens ...Cytokine & Growth Factor Reviews, 80 , 109–120. https://doi.org/10.1016/j.cytogfr.2024.09.003 (Link opens...

  12. Reflections and Looking Ahead

    Type
    Blog Post
    ...for antibody characterization. Nature Protocols. https://doi.org/10.1038/s41596-024-01095-8 Kahn, R. A....reproducibility in biomedical research. eLife, 13, e100211. https://doi.org/10.7554/eLife.100211...

  14. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    ...Review of Pharmacology and Toxicology 55:399–417 . https://doi.org/10.1146/annurev-pharmtox-010814-124803...Chemogenetics. In Methods in Enzymology (pp. 181–196). https://doi.org/10.1016/bs.mie.2018.01.022 Additional ...

  15. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...Protocols in Protein Science. John Wiley & Sons, Inc. https://doi.org/10.1002/0471140864 Fox, J. D., & Waugh...Protocols (Vol. 205, pp. 99–118). Humana Press. https://doi.org/10.1385/1-59259-301-1:99 Resources on ...

  16. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ...eukaryotic proteome? Open Biol. (2019) 9:180241. DOI: https://doi.org/10.1080/rsob.180241 Vanni, C., et al.,...coding sequence space. eLife. (2022) 11:e67667. DOI: https://doi.org/10.7554/eLife.67667 Springer, S. P. Gene...

  17. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ... human disease.     Sun, et al. bioRxiv. 2021. https://doi.org/10.1101/2020.05.04.076067    Novel fluorescent...Chesterfield et al., ACS Synthetic Biology 2020. https://pubs.acs.org/doi/10.1021/acssynbio.0c00192   ...

  18. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ... GPCR transducerome. Nat Chem Biol 16:841–849 . https://doi.org/10.1038/s41589-020-0535-8 Takeda S, Kadowaki...human genome sequence. FEBS Letters 520:97–101 . https://doi.org/10.1016/s0014-5793(02)02775-8 Additional...

  19. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...Targeted Neuronal Manipulation. Front Neuroanat 13: . https://doi.org/10.3389/fnana.2019.00093 Wilmott P, Lisowski... Virus. Human Gene Therapy Methods 30:206–213 . https://doi.org/10.1089/hgtb.2019.276 Additional resources...

  20. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    ...The Immunoassay Handbook (pp. 245–265). Elsevier. https://doi.org/10.1016/b978-0-08-097037-0.00017-8     .... 269, Issue 17, pp. 13048–13055). Elsevier BV. https://doi.org/10.1016/s0021-9258(18)99982-5 More resources...

  21. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...      Moore et al., ACS Synthetic Biology 202. https://doi.org/10.1021/acssynbio.0c00581   New tool ...Brookhouser, N., et al. BMC Biol 18, 193 (2020). https://doi.org/10.1186/s12915-020-00929-7    The CRISPR...

  22. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...research. View her research on her SSRN Author page: https://ssrn.com/author=1232466.     Neil Thompson is ...control trials in innovation. Visit his website at: http://www.neil-t.com/ .   References 1. Cong, Le, et...

  24. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...Relations Director for Curiosity Collider (hyperlink https://curiositycollider.org/) , which she co-founded...Outreach Manager for Science Borealis (hyperlink http://scienceborealis.ca/)    ...

  25. Plan Your Experiment

    Type
    Guide
    ... Methods in Molecular Biology , 1239 , 197–217. https://doi.org/10.1007/978-1-4939-1862-1_10 PMID: 25408407...Genetic Screens. Cell , 167 (7), 1853–1866.e17. https://doi.org/10.1016/j.cell.2016.11.038 PMID: 27984732...mammalian cells. Nature Protocols , 17 (11), 2431–2468. https://doi.org/10.1038/s41596-022-00724-4 PMID: 35941224...repression and activation. Cell , 159 (3), 647–661. https://doi.org/10.1016/j.cell.2014.09.029 PMID: 25307932...editing. Trends in Biotechnology , 36 (2), 173–185. https://doi.org/10.1016/j.tibtech.2017.11.006 PMID: 25307932...experiments. Nature Biotechnology , 38 (7), 813–823. https://doi.org/10.1038/s41587-020-0490-7 PMID: 29305085...genome editing. Scientific Reports , 5 , 11315. https://doi.org/10.1038/srep11315 PMID: 26066060 Zukher...

  27. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...vaccine development. Vaccines. 7, 75 (2022). DOI: https://doi.org/10.1038/s41541-022-00503-y Gorry PR, ...landscape. Sig Transduct Target Ther 6, 53 (2021). DOI: https://doi.org/10.1038/s41392-021-00487-6 Additional ...

  28. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...microscopic imaging. Nat Commun 11, 5979 (2020). https://doi.org/10.1038/s41467-020-19447-9 Hohlbein, J...: quo vadis?. Nat Methods 19, 1020–1025 (2022). https://doi.org/10.1038/s41592-022-01602-3...

  29. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ... system. Science, 343(6166): 80-84 (2014). doi: https://doi.org/10.1126/science.1246981. Leemhuis, H.,...strategies. IUBMB Life, 61: 222-228 (2009). doi: https://doi.org/10.1002/iub.165.  Additional resources...

  30. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...University Flow Core. Retrieved January 31, 2022, from https://www.bu.edu/flow-cytometry/files/2013/06/Fluorochromes-Brightness-Chart.pdf...Core Facility. Retrieved January 31, 2022, from https://flowcore.syr.edu/help/spectral-overlap/   More...
Showing: 151 - 180 of 258 results