We narrowed to 261 results for: Mac

1. Negative Can Be Positive: Open AAV Data with Addgene

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   ...214–225. https://doi.org/10.1089/hgtb.2019.105 Rumachik, N. G., Malaker, S. A., Poweleit, N., Maynard,...

2. An Introduction to Adenovirus

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   ... Bian, A., Zhi, Q. X., Iparraguirre, A., Lopez-Camacho, C., Wherry, E. J., & Ertl, H. C. J. (2007). Adenoviral...

3. Viral Vectors 101: The Retroviral Lifecycle

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   ...into the host cell’s genome and then use cell machinery for transcription and translation. Here, we will...

4. Simplify Cloning with in vivo Assembly

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   ..., but have successfully tested DH5a, TOP10, and Mach1. No common lab cloning strain has failed so far....

5. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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   ...have been developed to recruit transcriptional machinery to dCas9 and its RNA directed binding site. These...

6. AAVs in Retinal Gene Therapy

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   ...retinitis pigmentosa, achromatopsia, and age-related macular degeneration are ongoing and some will hopefully...

7. Tips for Screening with Yeast Two Hybrid Systems

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   ...: PMC2965261. 8. Obrdlik, P., El-Bakkoury, M., Hamacher, T., Cappellaro, C., Vilarino, C., Fleischer, ...

8. Plasmids 101: Protein tags

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   ...Guide Expression Vectors from the Berkeley QB3 MacroLab Eric thanks his wife, Annette Sievers, Ph.D.,...

9. New CRISPR Tools: Cas7-11 and PASTE

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   ...system wasn’t dependent on the cell’s intrinsic machinery being already activated to work. (It was also ...

10. Save Time with Transient Plant Leaf Transformations

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    ... a virus and the plant's post-transcriptional machinery to silence genes of interest (Unver and Budak,...

11. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

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    ...and historically, bagpipes were made using the stomachs of different animals. Check out the Addgene website...

12. How to Be an Excellent Trainee

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    ...maybe you would like to take pictures/videos of a machine set up while your mentor is demonstrating it. Requests...

13. Transferable Skills Guide: External Collaborations

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    ...become popular. There are only so many methods and machines one team can learn so linking together expert ...

14. Twenty Years of Sharing: Expanding Accessibility

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    ...Beijing Zhongyuan 2012 China Summit Pharmaceuticals International 2013 Japan Leehyo Bioscience...

15. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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    ...2008. 8(3): p. 147-61. PubMed PMID: 18537590. 9. Mack, A.A., et al., Generation of induced pluripotent...

16. Plasmids 101: Gateway Cloning

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    ...coli strain sensitive to CcdB (e.g. DH5α, TOP10, Mach1). The ccdB gene is present in the donor vectors ...

17. Hot Plasmids - August 2020

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    ...immobilized metal ion adsorption chromatography (IMAC) using Ni-NTA magnetic beads. Workflow of ...

18. Finding nucleic acids with SHERLOCK and DETECTR

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    ... Isern S, Michael SF, Lorenzana I, Yozwiak NL, MacInnis BL, Bosch I, Gehrke L, Zhang F, Sabeti PC (2018...

19. CRISPR-mediated Plant Base Editors

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    ...is either repaired by cellular mismatch repair machinery making an original C-G pair converted to T-A or...

20. Getting the Most from Your Lentiviral Transduction

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    ...Addgene's New Viral Service! References 1. Hay, R.J., Macy, M.L. & Chen, T.R. “Mycoplasma infection of cultured...

21. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

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    ...Griffith, her research focuses on the replication machinery of Kaposi’s sarcoma-associated herpesvirus.   ...

22. Plasmids 101: The Promoter Region – Let's Go!

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    ... in your host organism. Because transcription machinery differs between cell types or organisms, promoters...

23. Five Popular Model Organisms

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    ...of the fly in therapeutic drug discovery." Pharmacological reviews 63.2 (2011): 411-436. PubMed PMID: ...

24. Hot Plasmids: Summer 2024

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    ... determination on magnetic beads for scarce macromolecules in heterogeneous samples. bioRxiv 2024.01.21.576499...

25. Antibodies 101: Introduction to Gating in Flow Cytometry

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    ...expression, and viability. Gates are the parameters the machine uses to differentiate between variations in those...

26. Antibodies 101: Single Chain Fragment Variables (scFvs)

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    ...also used for eye diseases, such as age-related macular degeneration (AMD) since direction injection to...

27. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

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    ...highlight the specific modifications to CRISPR machinery that allow for the use of CRISPR in plants, and...

28. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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    ...hurdles. The first is the delivery of the CRISPR machinery to all of the target cells. This goal may prove...

29. Is this the right place for me? 8 tactics for choosing a lab

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    ... a mentor that sees you only as a paper making machine. Choose one that is a true mentor, supporting career...

30. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

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    ...When delivering the ssDNA template and CRISPR machinery, Quadros et al. recommend ribonucleoprotein (RNP...

31. Technologies Enabled by NanoLuc® Luciferase

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    ...27966527. PubMed Central PMCID: PMC5171807. 6. Machleidt, T., et al. (2015) NanoBRET-A novel BRET platform...

32. CRISPR 101: Epigenetics and Editing the Epigenome

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    ...Both of these methods also recruit epigenetic machinery - but is there an advantage to using direct epigenetic...

33. PiggyBac-ing Through the Genome Editing Field

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    ...the piggyBac™ donor is used by the cell’s host machinery for DNA repair by homologous recombination, which...

34. Building Global Connections with the International Mentorship Program USA-EUROPE

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    ... Medical School, where she studies age-related macular degeneration. She is also a blogger who writes ...

35. Live and Let Dye: Self-Labeling Protein Tags

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    ..., Frangieh, C. J., Friedrich, M. J., Faure, G., Macrae, R. K., & Zhang, F. (2023). Cell type-specific ...

36. Quick Guide to All Things Lentivirus

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    ...quiescent or terminally differentiated cells such as macrophages and microglia. This property makes the lentivirus...

37. Finding Your Science Policy Path

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    ...implementation, program management, communications, diplomacy and international relations, advocacy, and writing...

38. Plasmids 101: Modular Cloning Applications and Kits

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    ...a broad range of parts, including copy number machinery, chromosomal integration modules, and many more...

39. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

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    ...Brown, T. A., Lemon, W. C., Patel, R., Lu, R., Macklin, J. J., Keller, P. J., Ji, N., & Lavis, L. D. (...

40. With an Eye Towards the Future, We Look Back at the March for Science

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    ...where these issues hit hardest. According to the Guttmacher Institute, most women of reproductive age live...