... cell from the clinic. The 2nd generation is similar to the first one with the exception that the HIV...must be chosen carefully. Often researchers want a simple set-up where the gene of interest is expressed ...experience in transfection, CaCl2 transfection is a simple and cheap technique that should work well. Quickly...

...pitfalls of accepting alternative facts. Because, similar to scientists, the legacy of each government and..., challenged us. I worried the march would be a simple, unquestioning celebration of science, at a time...research scientist would be a career that could simply cease to exist. But then November hit and panic...

... every genomic target. Due to its comparative simplicity and adaptability, CRISPR rapidly became the most... change the genomic target of the Cas enzyme by simply changing the target sequence present in the gRNA...The requirements for a CRISPR knockout assay are simple. You’ll need a Cas enzyme and a gRNA specific to...AcrVA3.1, which inhibits both Cas12a and Cas3. Similarly, some Acr proteins are specific to a particular...editors are based on cytosine base editors and similarly work by first converting cytidine to uridine. ...repress gene expression in mice and human cells. The simplest dCas9-based activators and repressors consist ...imaging has numerous advantages, including the simplicity of gRNA design, programmability for different...

...likely to express the target at a robust level, similar to that in samples of interest. If you are working...   If your protein of interest is expressed at similar levels in your cell lines, you can consider a cell...transiently overexpressed in CHO cells and displayed similar labeling patterns on different primary blood leukocytes...

...audience blindly receiving them; it results in highly similar content and the absence of iterative design; it...published 1 Jan 2013, no longer available), is a simple catalogue of reported sgRNA designs. The second...The third, WGE (published 15 Sep 2015), is more similar to EENdb than CrisprGE, but is also a good example...

...a single recombination reaction, drastically simplifying the process and reducing the amount of time required...specific recombination sites (in this regard, not so dissimilar from restriction enzyme cloning). To do this ...selection cassettes). Although gateway cloning is a simple and efficient cloning method, gateway vectors and...benefit of Gibson cloning is that it allows for the simple assembly of multiple fragments of DNA in the chosen...Oligonucleotide Stitching Yeast-mediated cloning is very similar in principle to Gibson cloning, but instead of ...the powerful recombination abilities of yeast. Similar to Gibson, this method can efficiently fuse two...

...protein that enables imaging of two biosensors simultaneously   Researchers in the labs of Michael Lin ...mCyFP1 and EGFP as FRET donors in experiments simultaneously measuring the activities of CaMKII and RhoA...PhoCl between an enzyme and an inhibitor domain similarly allows for control of enzymatic activity. In addition...

... of the best ways to learn about a new field is simply to know where to look and how to start. There is...  About the author Niko McCarty is the editor of Asimov Press and is designing a genetic engineering curriculum...

...structures demonstrating “the structure is strikingly similar to the design model at atomic resolution” (Kuhlman... design is possible. Although Top7 was designed simply to be stable and novel, the principles that created...

...in the biological sciences and techniques that simplify and expedite research. We recognize, however, ... blog! To sign up to write for the Addgene blog simply email us. We can’t guarantee that all those who...

...that within that article you’ll find new plasmids. Simply click on the badge to be taken directly to the ...an account on PubMed. Once you have an account, simply click on your account name in the top right corner...

...application. The submission process is easy, with simple, descriptive fields, drop-down boxes when appropriate...can work through it at your own pace. To submit, simply click "Contribute Data."    Figure 6: Contributing...

...ladders are not next to empty wells. Most people will simply load an extra ladder or technical replicate (ie... leftover sample) to fill the relevant wells. Similarly, if you load a gel and then forget to start it...blocking time! Fill the time with smaller tasks, or simply enjoy some well-deserved down time while waiting...

...Addgene, we try to make the process as smooth and simple as possible. We are the Customer Support Specialists... Placing an order on Addgene’s website is quite simple, even if you’re using a PO number. Follow these...

...advantage of KTRs is that they are easy to create and simple to multiplex. Problems with FRET kinase reporters...swapping of fluorescent proteins and multiplexing is simple when fluorescent proteins with limited spectral...

...DePace’s yearly planning meetings revolve around two simple worksheets, each of which is filled out separately...Extracurricular Activities for a Strong Science Career Path 7 Simple Strategies to Resolve Conflict With Difficult Supervisors...

... it to fruition. The concept, though relatively simple, changed the course of scientific research. What...of requesting and sharing plasmids has become simplified through Addgene. Looking back, Addgene’s journey...

...cerevisiae and S. pombe. The protocol is surprisingly simple, requiring only a PCR product containing the modifying...ensure that all desired tags have been integrated. Simply design your amplification primers with the desired...

...green fluorescent proteins. nLightR did perform similarly to GRABNE, and having both colors allows for increased...AR. Selectivity Norepinephrine and dopamine are similar molecules, and cross-reactivity is a very common...

...this simple act directly associates you with a network of researchers who may be working on similar projects...

...this over 30 minutes, which was fun to watch. A simpler 1 protein - 1 RNA system Drs. Dounda and Charpentier...into one RNA to direct cleavage the result was a simpler 1 protein - 1 RNA system.  Dr. Charpentier advocated...

...designed in the same vector backbone with very similar restriction enzymes. You can essentially cut and...quickly. Researchers should keep in mind that a very simple sequential or double restriction enzyme digest ...

...spent collecting it will have been worthless. But simply writing down your data doesn’t guarantee a publication...welcoming us. Then it was time to work! Set up similarly to the online class, we met as a group for sessions...

...analyzing deposits with many plasmids that contain similar features and it allows you to more quickly identify...size of the plasmid and the insert. By using the “Simulate agarose gel” option with the desktop version of...

...such as ChR2 and NpHR, can even be expressed simultaneously and still be individually activated with different... By looking at groups of neurons that elicited similar tail movements, they were able to narrow down the...

...antibodies in the serum of a camel and soon after found similar antibodies in llamas and alpacas (Figure 1). At...Suitability for in vivo experiments, due to their simple structure    sdAbs in research These advantages...

...even blogged about this here and here); however, simply put, I moved into this position because I want ...will find useful and interesting than that will simply get people ordering plasmids. The need to keep ...

...biosensor is the GFP-LC3-RFP-LC3ΔG probe. It’s similar to the mRFP-GFP-LC3 Tandem Fluorescent Protein,...fluorescent protein oligomerization Seeing red: simple GFP photoconversion Light sheet fluorescence microscopy...

...e.g. titer, injection site, animal strain used). Similar to the goodwill of Addgene plasmid depositors, ...this resource for the community. There are a few simple steps you can take to input your data for sharing...

...target protein? Epitope Availability The answer is simple: epitope availability. An antibody can only bind...that has been validated for an assay that uses similar sample preparation - for instance, if you cannot...

... useful natural materials, such as antibiotics. Simon Moore and Paul Freemont’s lab previously developed..., this cell free system allows for a relatively simple, controlled “one-pot” reaction capable of reproducing...

...probed simultaneously on the sample. What to do?  If you’ve ever found yourself facing a similar problem...

...problem, Gao et al. searched for Argonaute orthologs similar to TtAgo and PfAgo, another thermophilic Argonaute...variety of mammalian cell lines, with an efficiency similar to that of Cas9. Like Cas9, NgAgo is also able ...

... out more than necessary for my interview. The simplest place I could think of to start was “Marketing...about marketing would be to start reading a broad simple overview, learn some jargon and key phrases and...

...Merkx of Technische Universiteit Eindhoven has similar interest in FRET-based sensor proteins, particularly...monitoring calcium versus zinc should require the simple swapping of binding domains, in practice optimizing...

...hopes that other researchers will be able to use similar designs to create a wide variety of enzymes and...breaks down the HMO of interest into its component simple sugars (including lactose), and a lac operon controlled...

...sequencing instruments by enhancing the efficiency and simplicity of library prep. As part of our mission, we’ve...here is that, as more samples are sequenced simultaneously, it gets harder and harder to obtain balanced...

...cell and any individual AAV particle is rare. But simply getting into the cell may not always be enough ... can optimize AAV production. We’ve listed some simple steps you can take below but you should read our...

...a white color. While CRISPRainbow allows for simultaneous visualization of sites on different chromosomes... color detection In order to label two loci simultaneously, Ma and Pederson created another sgRNA containing...

...produced? Purifying exo-AAVs is actually a bit simpler than the classical purification protocol involving...summary, exo-AAVs seem to target cell types with a similar specifically as conventionally produced AAV particles...

...feasible, three separate things happened nearly simultaneously: The Wellcome Trust funded a center for mitochondrial... process of good research practice: computer simulations, in vitro research, animal models, and several...

...dilution factor x 50 ug/ml This method is quick and simple and doesn’t require any special reagents. However...Quantifying DNA using capillary electrophoresis is similar to quantifying DNA using gel electrophoresis. Except...

...prime editing, achieving up to 3-loci MPE with similar high efficiencies, which is scalable for a higher...adeno-associated virus (AAV) and lentivirus, demonstrating simultaneous editing of genes against multiple genetic diseases...

...certain projects. The idea behind the platform was simple: any person working on COVID-19 could file a request...groups. In other cases, the collaborations were simpler and the volunteers helped with things like translations...

...agreement, read on!  What is the MTA?  An MTA is simply a contract or agreement that states the terms of...clearly communicated. And that’s where MTAs, and similar agreements, shine.  These types of agreements allow...

...an invaluable tool to be used in this capacity. Similar to the Human TFome Library, the MORF library can...understanding of disease onset, but also better simulate a disease in a model system. TF screens can be...

... you have something to ask or say. It can be as simple as “it was nice meeting you.” Include follow-up...person on LinkedIn I once helped by answering one simple question so I accepted the connection invitation...

...pocket.   5. +AMINO- by iGreenEarth AMINO is a very simple app that packs important biological information... names have a B, J, X, or Z. Some alternative, similar apps: Findings Lab Notebook Cell Culture Split...

...using CRISPR/Cas9 for genome editing, researchers simply need to express a gRNA designed to direct Cas9 ...these arrays, they did not know their functions and simply called the repeated elements “direct repeats” and...

...BamHI) are capable of cleaving sequences which are similar, but not identical, to their defined recognition... the DNA mass of comparably intense samples of similar size. Run the gel at 80-150V until you have good...