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We narrowed to 569 results for: CIT

Showing: 101 - 120 of 569 results

  1. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...AAV components      Cargo capacity Empty rAAV has a cargo capacity of ~ 4.7 kb, meaning that your...or not infect) different cell types with some specificity. There are a lot of AAV serotypes, many of which...or 2A to co-express them from one promoter. Specificity The ability of AAV to drive gene expression in...or tissues is one of its biggest advantages. Specificity can be achieved using specialized promoters, ..., and/or serotype can also be used to ensure specificity; this is particularly helpful when wanting to...

  2. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    ...the host genome, removing the risk of oncogenicity or genotoxicity seen with other vectors (Lukashev et...large transgene packaging capacity. For instance, with a packaging capacity of about 6 kb, the 1st generation...AdVs have already a packaging capacity larger than AAV vectors. This capacity is large enough to carry the...

  3. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    ... permanent residence case with United States Citizenship and Immigration Services (USCIS). If it were ...researchers who require visas, permanent residence or citizenship to continue their work in the US have the added...objective criteria including awards, media attention, citations to your work, downloads, commercialization of ...to join (selected due to expertise in field). Publicity in major media and/or trade journals: You the ...platinum standard for an expert is someone who has cited your work. Immediate steps that you can take to ...working visas, permanent resident green cards and citizenship.   Lauren Celano, CEO Propel Careers. Lauren...about working with scientific individuals to find exciting growth opportunities in life sciences       Propel...

  4. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...regeneration into one of my own courses, I was excited to hear about how he developed his program. Initially... students work on malate dehydrogenase (MDH), a citric acid cycle enzyme he studied during graduate school... to identify regions of MDH that interact with citrate synthase and presented their findings at the American...the research process – so if this sounds like an exciting opportunity for your classroom, I highly recommend... Wallert, M., Provost, J. 2010a. Bringing the excitement and motivation of research to students; Using... Wallert, M., Provost, J. 2010b. Bringing the excitement and motivation of research to students; Using...

  5. CRISPR Antimicrobials

    Type
    Blog Post
    ...induce cell death. CRISPR also enables increased specificity - rather than targeting a process essential to...like for organisms like C. difficile. In 2014, Citorik et al. and Bikard et al. developed sequence-specific...antimicrobial, plasmid-based, CRISPR systems (Find the Citorik Plasmids here). In both cases, they used 1-2 CRISPR...sequences found only in certain bacteria (Figure 1). Citorik et al. targeted beta-lactam and quinolone resistance...beta-lactam resistance genes, again with very high specificity. This double phage system both favors antibiotic...fight against bacterial superbugs. References 1. Citorik, Robert J., Mimee, Mark, and Timothy K. Lu. “Sequence-specific...

  6. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...ssAAV). To maintain the packaging capacity and improve the specificity of ssAAV, a targeting sequence of...As research has just begun with PE7, it will be exciting to see future studies enabled by this tool as ... in an AAV vector (Figure 5). Efficiency and specificity of these AAV vectors for microglia was further...mice. B) Quantifications showing high microglia specificity of GFP expression for the indicated ssAAV5s. ...reconstitute the gene of interest, reducing the immunogenicity typically associated with viral vector-based...

  7. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ... humanities, and 32% in the social sciences go uncited. Creative titles and controversial topics can garner...since the 1980s researchers have been increasingly citing research from outside of their disciplines. Included...at the same time increasing interdisciplinary citations in their own work. Of course not all research ...ImpactStory, a website that helps researchers go beyond citation rates to measure the impact of their contribution... the WeShareScience project is to find new and exciting ways to share ideas derived from research. The...Archambault. "The decline in the concentration of citations, 1900–2007." Journal of the American Society for...

  8. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ..., you can easily directly excite the acceptor with the laser used to excite the donor. In that case the... energy is transferred non-radiatively from an excited molecular fluorophore (the donor) to another fluorophore...between the two fluorophores is linked to their excitation spectrum overlap. If you choose a pair too close...sensitised emission method, where the donor is excited by a specific wavelength of light and the signal...method is also less sensitive to direct acceptor excitation artifacts and it is possible to use a non-fluorescent...

  9. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...promoters with no tissue specificity, so in some cases they can lead to toxicity and off target effects.... flexibility in expression levels and tissue specificity. Using your shRNA plasmids One of the most common...color":"#0052a9","css":"#0052a9","hex":"#0052a9","opacity":100,"rgb":"rgb(0, 82, 169)","rgba":"rgba(0, 82..."css":"rgba(0, 82, 169, 75%)","hex":"#0052a9","opacity":75,"rgb":"rgb(0, 82, 169)","rgba":"rgba(0, 82,...color":"#ffffff","css":"#ffffff","hex":"#ffffff","opacity":100,"rgb":"rgb(255, 255, 255)","rgba":"rgba(255...

  10. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...Bicistronic vectors combine excitation and inhibition by using both excitatory and inhibitory channels activated...Light activated ion channels (rhodopsins) either excite or inhibit neuronal activity upon exposure to light...light within a specific range of wavelengths. Excitatory channels allow positively charged ions such as...wavelength, turns it off, allowing the cell to be excited over a sustained period of time (Gong et al., 2020... the opsin.      Figure 2: Diagram of excitatory (ChR2) activation by light showing ions flowing...

  11. Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies

    Type
    Blog Post
    ...proteins and this interaction determines the specificity and efficiency of the AAV serotype used for a...Combining different approaches can increase the specificity of cell targeting even further. For example, ...AAV at the injection site results in increased specificity of the targeted cell population.  Combinatorial...improve delivery by increasing transgene cargo capacity, enhancing anterograde transport efficiency, and...testament to openness and sharing in science.  We’re excited to support the neuroscience community by sharing...

  12. Antibodies 101: Validation

    Type
    Blog Post
    ...arrived, hooray! Before you jump straight into the exciting new experiments that you’ve been planning, it ... to molecular targets with high affinity and specificity. However, there are countless variables that ...that impact an antibody’s affinity and specificity for a given target and it is all too common for antibodies...experiments for every antibody that you receive; citing existing validation data can be acceptable. However...binds to what you expect it to bind to. Show specificity for the target antigen - Just because an antibody...something unexpected. This approach has high specificity, but you need to be aware of any possible alternative...

  13. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...Being excited about the science allows you to ask more interesting questions and your excitement will ...us everything there is to know is a strange and exciting revelation. Science in any field can generally...vague sense of what kind of research you would be excited about. I know quite a few friends who opted to ...people actively seek out science that genuinely excites them, and reach out to the PI to get involved. ...their research, and learn about what makes them excited to do what they do. I would also highly recommend...

  14. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ...Antibody dependent cellular cytotoxicity (ADCC) Antibody dependent cellular cytotoxicity (ADCC) is a slightly...deposition (ADCD) b) antibody-dependent cellular cytotoxicity (ADCC) c) antibody-dependent cellular phagocytosis...JV. Inhibitory Fc receptors modulate in vivo cytotoxicity against tumor targets. Nat Med. 2000 Apr;6(4... constant region contributes to affinity and specificity. Trends in Immunology, 29(2), 91–97. https://...

  15. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type
    Blog Post
    ...future of open science are you most excited about? Katz: What’s exciting about the open science movement ...We’re excited to welcome Karen Katz, Esq and Carl Paratore, MBA, CPA to the Addgene Board of Directors... will accomplish in the near future? Katz: I’m excited that Addgene is continuing to look at providing... General Auditor at Tufts Health Plan. In that capacity he leads the Internal Audit function and is responsible...

  16. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...hiring again and getting ready to embark on new exciting projects. Right now we don’t have to worry about...will have some fun challenges this year and I’m excited about this, but definitely not worried. I have ...the way scientists starting their own labs get excited about making their first deposit to Addgene. ...law review article we received some entirely unsolicited praise: “Addgene, for one, is a sterling example...have so few worries and can often focus on the excitement of what we are accomplishing and what more we...

  17. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...most of the alternative reagents have reduced immunogenicity. Effector functions are eliminated, as is antibody...reduced binding strength, half-lives, and/or specificity as well. They’re more difficult to source, may... typically consist of 3-4 repeats, have high specificity and binding affinity, can be produced in E. coli...generated using combinatorial libraries, and high specificity has been reported for a number of targets. Due... reagents you use, one thing is clear: it’s an exciting time to be a scientist looking for that perfect...

  18. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...variety of ways. First, review the citations. An antibody that has been cited thousands of times may be a safer...Picture this: you’ve been assigned an exciting new project aimed at understanding how a critical cellular...similar to yours. Take a look at the publications cited on the manufacturer’s website, which may contain...secondary considerations to validation, application specificity, and experimental considerations - no other factors...

  19. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    ...We are excited to announce the recipients of this year’s Michael Davidson and Roger Tsien Commemorative...cultured neurons, imaged 14 days post-transfection, excited at 505 nm (right). Scalebar represents 100 µm. ...painful stimuli are processed by a network of excitatory and inhibitory neurons whose function and connectivity...mammalian cell line engineered to be electrically excitable. The mutants are co-expressed with a photoconvertible...membrane voltage,” says Tian. Figure 5: Citrine-tagged voltage indicator expressed in mouse cortex...

  20. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    ...-invasive gene delivery and optogenetic excitation. Excitation of neurons deeper in the brain may still...makes them light-responsive. When light shines on excitatory channelrhodopsin-expressing neurons, the channelrhodopsins...action potential (Figure 1). This light-induced excitation can take place in a tissue culture dish but can... as compared to direct injection. This lower multiplicity of infection (MOI) makes it challenging for ...
Showing: 101 - 120 of 569 results