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...the biology of C. elegans and other nematodes. Wormbuilder - Describes methods to engineer the genome of...characterized which allow researchers to express desired constructs in precise cell types and/or developmental...that contain tools for use in worm research. This includes CRISPR, RNAi, empty backbones, fluorescent proteins...expression plasmids. Large collections or kits are described below and not included in this table. For a more...search Addgene's entire collection. ID Plasmid Description Gene/Insert PI Plasmids Expressing Worm Genes...strains. CRISPR plasmids from John Calarco's lab as described in Heritable genome editing in C. elegans via ...genome-editing events. Developed in Craig Mello's lab and described in A Co-CRISPR Strategy for Efficient Genome Editing...

...Bacteria CRISPR plasmids designed for use in bacteria. C. elegans CRISPR plasmids designed for use in C. elegans... CRISPR plasmids designed for use in Drosophila . Mammalian CRISPR plasmids designed for use in mammalian...Parasites CRISPR plasmids designed for use in parasites. Plant CRISPR plasmids designed for use in plants. Yeast...Yeast CRISPR plasmids designed for use in yeast. Xenopus CRISPR plasmids designed for use in Xenopus . ...Browse depositor protocols, find software for gRNA design and deep sequencing analysis, discover links to.... Zebrafish CRISPR plasmids designed for use in zebrafish....

... (V70) (Plasmid #188537) . These reagents are described in detail in Mohler et al. (2023) and help deliver...purifying the phosphosite library as GST-fusion peptides (“Mode #1” expression). The Mode #1 phosphosite...Phosphorylated Isomers (iSPI) The iSPI resource described in Galloway et al. (2022) is adapted from the ...the synthetic human phosphoproteome. It subdivides the 110,139 human phosphoserine site library (Pooled ...Iterative Synthetically Phosphorylated Isomers (iSPI) described in: Gassaway, B. M., Li, J., Rad, R., Mintseris...in a new window) rEcoli XpS and a new pSerOTS described in: Mohler, K., Moen, J. M., Rogulina, S., & Rinehart..., 551 KB) Human Serine Phosphopeptide Library described in: Barber, K. W., Muir, P., Szeligowski, R. V...

...Empty Backbones Plasmid Description Industry Return to top Blue Plasmid Description Industry Return to top...top Cyan Plasmid Description Industry Return to top Green Plasmid Description Industry Return to top mEmerald... mEmerald Plasmid Description Industry Return to top Yellow Plasmid Description Industry Return to top...top Orange Plasmid Description Industry Return to top Red Plasmid Description Industry Return to top Photoactivatable...photoactivatable fluorescent proteins. Plasmid Description Industry Return to top Content last reviewed:...

... is described in more detail in the Multiplex Assays section below. The following table describes the ... Science Guides Antibody Guide Antibody Guide View Addgene's Antibody...of B cells isolated from animals immunized as described above are used to create hybridomas, which produce...each antibody has two binding sites. Isotype Description Configuration and Valency Subclasses IgA Prevalent... used to quantify relative amounts of protein. Includes: Western blot ELISA Capture - used to isolate ...isolate specific proteins or protein complexes. Includes: Immunoprecipitation (IP) Chromatin immunoprecipitation...Visualization - used to visualize protein location. Includes: Immunofluorescence (IF) Immunohistochemistry ...

... Linzhao Cheng 17452 pLenti CMV Puro DEST 3rd Gateway destination plasmid with constitutive or inducible... for gRNA expression . ID Plasmid Generation Description PI 8453 pLKO.1 puro 3rd U6-driven shRNA empty...cloning plasmid 3rd U6-driven shRNA empty plasmid. Includes a stuffer for easy cloning. David Root 14748 pLKO...Fraser 15948 pLOVE 3rd Tet inducible gateway destination plasmid for cDNA expression. Miguel Ramalho-Santos...Tet-inducible lentivirus for ORF expression, multicistronic design enables tracking of viral transduction. See plasmid... based) for inducible knockdown, multicistronic design enables tracking of viral transduction. See Meerbrey...Tobias Meyer 86849 pBOB-EF1-FastFUCCI-Puro 3rd Encodes FUCCI reporters for cell cycle analysis. Puromycin...

... 36991 Desmoglein2-mCherry Desmosomes Desmoglein2 mCherry Kathleen Green 36989 1105-Desmoglein2-FLAG-eGFP... PIP2a mCherry Maria Harrison 61206 pCMU-PDESr Plasmodesmata (Plants) PDLP1 mCherry Maria Harrison 61200...-eGFP Desmosomes Desmoglein2 EGFP Kathleen Green 74260 pKanCMV-CX4.3-7aa-mClover3 Gap junctions Connexin...Davidson 40907 pDEST/hCx43-EGFP-N1 Gap junctions Connexin 43 EGFP Robin Shaw 49861 pDEST-mCherry-GJA1-20k-N1...Actin filaments beta-Actin EGFP Robert Singer 40908 pDEST/LIfeAct-mCherry-N1 Actin filaments LifeAct mCherry...junctions, gap junctions, tight junctions, and desmosomes. Browse or search the table below to find markers...

...at the bottom of this page. For more in depth descriptions of each serotype, check out Addgene's blog on...Browse Available PHP.eB AAV ID Name Promoter Description Category PI Controls 28306 pAAV-FLEX-tdTomato... Browse Available PHP.S AAV ID Name Promoter Description Category PI 28306 pAAV-FLEX-tdTomato CAG tdTomato...Browse Available PHP.V1 AAV ID Name Promoter Description Category PI 104052 pAAV-CAG-DIO-EYFP CAG EYFP...Browse Available MaCPNS1 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control...Browse Available MaCPNS2 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control...Browse Available CAP-B10 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control...

...can be designed using Benchling or using similar design tools and ordered as standard desalted oligonucleotides...phosphorylated oligonucleotides containing the guide target sequence: Up to two guides can be expressed...that utilize Golden Gate cloning to insert the desired guide RNA sequence by digestion of the backbone... #63798) Plasmids from Collection ID Plasmid Description PI Publications Kaufman, M. L., Goodson, N. B...

...efficiency, selecting desired clones, and making transgenic mice. ID Plasmid Description Gene/Insert Vector...light”): Cre Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene resources...Return to top Flp Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene resources...Return to top Dre Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene resources...Site-Specific Recombinase Plasmids ID Plasmid Description Gene/Insert Vector Type Promoter PI Return to...target site (lox, FRT, rox, etc.) ID Plasmid Description Vector Type PI Additional Addgene resources Use...Pooled Libraries Pooled Library Name Type PI Description Mouse Tumor Suppressor Gene CRISPR Knockout Library...

... Large collections or kits are described below . ID Plasmid Description Gene/Insert PI Return to top Highlights...rerio ), named for the horizontal stripes on the sides of the adult body, is a tropical freshwater fish...contain tools for use in zebrafish research. This includes plasmids for genome editing, empty backbones, ...Periklis Pantazis Lab Multibow: digital spectral barcodes for cell tracing - Sean Megason Lab Zebrabow -...Du Lab. A set of pCS2+ backbone-based Gateway destination vectors (pGCS), bearing either amino- or carboxyl-terminal...promoters, six fluorophores, and empty vectors designed to streamline the production of transgenic zebrafish...zebrafish-specific gene expression database that provides information about when genes are active in developing...

...scientists. Antibodies ID Recombinant Antibody Description PI 211756 Anti-SARS-CoV-2 Nucleocapsid Protein...secretion, and codon-optimized versions designed for your expression system. Some of the following...Plasmid Distribution to Industry page. ID Plasmid Description Industry PI Additional Addgene resources SARS-CoV...SARS-CoV-2 Viral Pseudotyping Collection – This page includes a table of all spike expression plasmids in our... SARS-CoV, MERS, BAT-CoV Plasmids ID Plasmid Description Industry PI Due to export control regulations...plasmids related to these genes below. ID Plasmid Description Industry PI Additional Addgene resources ACE2...Neutralizing nanobody plasmids And more! ID Plasmid Description Industry PI Addgene blog Finding nucleic acids...

...brain cells and circuits during behavior. Long desired by researchers, the revolutionary dynamic picture... with Addgene materials. Plasmids ID Plasmid Description PI Viral Preps Addgene distributes ready-to-use...Learn more about our viral service ID Viral Prep Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine...and proximal dendrites in a viral vector Hillel Adesnik 107708-AAV9 pAAV-hSynapsin- soCoChR-GFP A somatic...nuclear mRuby3 by P2A in a viral vector Hillel Adesnik 109048-AAV9 pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1...ribosome entry sequence in a viral vector Hillel Adesnik 111066-AAV1 pAAV-hSyn-dLight1.1 To generate Adeno-Associated...photoswitchable control of inhibitory GPCR signaling cascades. Michael Bruchas 139505-AAV8 pAAV-Ef1a-DIO-PPO-Venus...

.... The longer pegRNA also encodes a primer binding site (PBS) and the desired edits on an RT template. ...post . Need help designing a pegRNA? Try the DeepPrime (Link opens in a new window) design tool from Hyongbum...pegRNA). As with a typical gRNA, the pegRNA is designed with a spacer that binds to a specific genomic...3’ flap binds to the PBS of the pegRNA and the desired edit is incorporated into the DNA by reverse transcription... CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...

...promoters (sometimes generally called pTet) have been designed by combining tet O sequences with elements like...plasmids use "TetO" or "Tet-O" in their name or description, meaning they contain a TRE or tet O and can ...as much flexibility and control as a dual vector design, but it is easier to implement and ensures that...transduction (transient or stable) may depend on the desired sensitivity, level of induction, or amount of background...expression of your gene of interest. ID Plasmid Description Transactivator Promoter PI 21915 Tet-pLKO-puro...Lentiviral Tet-On plasmid for easier cloning and design of inducible shRNA expression, with puromycin selection...transactivator for your tetracycline system. ID Plasmid Description Transactivator PI 26429 pLenti CMV rtTA3 Blast...

...genome-wide knockout gRNA pooled library designed with 10 guides against 8,158 predicted protein-coding ...CRISPR expression plasmids that have been designed for use in parasites. CRISPR...have been the focus of biomedical research for decades. Recent developments with CRISPR-Cas9 gene editing...Addgene's parasite CRISPR plasmid collection also includes the Toxoplasma CRISPR Knockout Pooled Library ... CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...

...This page lists plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays...Expression Vectors This page lists plasmid vectors designed to express nucleases in different cell types. ...zebrafish (can be used as a substitute for pMLM335 described in Foley et al., PLoS One 2009) pMLM292 (for expression...zebrafish (can be used as a substitute for pMLM336 described in Foley et al., PLoS One 2009) pMLM800 (for expression...Methods 2011 ) in mammalian cells or zebrafish as described by Moore et al. PLoS One 2012 and Cade et al.,...

...target sequence and RISC cleaves and degrades the mRNA. RISC includes argonaute protein 2 (Ago2), which ...RISC). RISC unwinds the double-stranded siRNA and degrades one of the strands. The remaining single-stranded... binding of RISC. For more information on shRNA design and delivery, see resources below. Figure 1: Overview... Trono Lab Lentivectors Toolbox Optimized shRNA design in pSico with pSicoligomaker (Andrea Ventura lab...Taxman, D. J. (2010). Short hairpin RNA (shRNA): design, delivery, and assessment of gene knockdown . Methods...

...light microscopy. In brain tissue, these protein barcodes allow for efficient machine-learning segmentation...enhancers for morphological readout. How to Use PRISM Barcodes are delivered as pooled AAVs either directly to...bias in packaging representation. To read out the barcodes, each protein tag can be imaged with a corresponding...combinations of protein bits. B) Multiplex imaging of barcodes with expansion microscopy and reconstruction of...Sheridan et al. (2025). PRISM Plasmids ID Plasmid Description Tags Additional Resources PRISM was developed...., & Payne, A.C. (2025). Combinatorial protein barcodes enable self-correcting neuron tracing with nanoscale...

...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR system has been...plasmids and protocols needed for using them, are described below. Read our CRISPR 101 post for more information...information about HDR/repair template design. How to use CRISPR to tag your gene of interest Mendenhall and...interest is currently unavailable, you will need to design and clone in a gRNA(s) to guide the Cas9 protein...protein to your target sequence, as well as design and clone in the homology arms for the donor plasmid. The...produces a single plasmid targeting vector that encodes both a guide RNA transcript and a repair template...