We narrowed to 804 results for: Eng
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TypeCollection...gondii Trypanosoma sp. Plasmids for Viruses Species Dengue Hepatitis Influenza Mumps virus Rubeola (measles...Read our CRISPR Methods for Bacterial Genome Engineering blog post to see how Addgene depositors are making...
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Zebrafish Plasmid Collection
TypeCollection...and non-coding RNAs in zebrafish - Tatjana Sauka-Spengler Lab Lineage Tracing Cell lineage tracing in multicellular...drivers in vivo. Biotagging toolkit - Tatjana Sauka-Spengler Lab. A genetic binary system for cell- and compartment-specific... -
Open Enzyme Collection
TypeCollection...functionality or add new functions through genetic engineering and directed evolution. More information about...polymerase 165543 pOpen-Bstpol Bst DNA Polymerase, Full Length 165544 pOpen-T5gene122 T5 DNA Polymerase 165553... -
COVID-19 Resources
TypeCollection...Finding nucleic acids with SHERLOCK and DETECTR . Feng Zhang's lab at the Broad Institute has utilized ...Center The Lancet COVID-19 Resource Centre The New England Journal of Medicine Coronavirus webpage PLOS Journals... -
CRISPR References and Information
TypeCollection... Manuel Leonetti Lab . For more information, see Peng et al. (2024) (Link opens in a new window) . gRNA...new window) A Google groups forum started by the Feng Zhang Lab . Protocols Lab(s) Description Plasmids... -
Genomic Deletions in Mammalian Cell Lines
TypeCollection...re-purposed for site-specific eukaryotic genome engineering. CRISPR/Cas9 is an inexpensive, facile, and efficient...manuscript here: Bauer et al, 2014 322.2 KB Genome engineering using the CRISPR-Cas9 system. Ran FA... -
CRISPR Pooled gRNA Libraries
TypeCollection...192824 Knockout Mouse Knouse 3rd ~5 71,878 Mouse messenger-RBP sgRNA Library 169082 Knockout Mouse Turner...Knockout Yeast Borodina N/A 1 56 Turner Lab Human messenger-RBP sgRNA Library 168791 Knockout Human Turner... -
Plasmids for Stem Cell Research
TypeCollection...Cells Transl Med. 2015 Mar 5. pii: sctm.2014-0214. Cheng RNA Human Reprogramming factors for delivering synthetic...expression signatures. Cell Res. 2011 Mar;21(3):518-29. Cheng piggyBac Mouse Doxycycline-inducible piggyBac transposon... -
Tetracycline Inducible Expression
TypeCollection... control gene expression, the system has been engineered with a variety of improvements. A synthetic Tet...systems (Loew et al., 2010; Heinz et al., 2011), engineered to eliminate binding of endogenous mammalian ... -
Fluorescent Protein Guide: Subcellular Localization
TypeCollection...fluorescent protein that has a different emission wavelength. For example, if the marker protein is fused ...Patricia Wadsworth 27123 eTC GFP beta-actin full length Actin Filaments beta-actin EGFP Robert Singer 27382... -
Fluorescent Protein Guide: Empty Backbones
TypeCollection... Gene, 1992 ), and since then scientists have engineered numerous GFP-variants and non-GFP proteins that...Photoactivatable (e.g. off to on) Excitation and Emission wavelengths after activation. Protein Excitation (nm) Emission... -
Genetic Code Expansion
TypeCollection...Because there are no free codons, this can be challenging. In E.coli , the rarest codon is the amber stop...the ncAA in the media to make sure that the full length protein is only made when the ncAA is included.... -
Synthetic Biology - Browse Plasmids
TypeCollection...SynBio Assembly Standards SynBio Blog Genome Engineering Browse the list of available plasmids related... -
Synthetic Biology - Algal
TypeCollection...SynBio Assembly Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic biology... -
Synthetic Biology - Bacterial
TypeCollection...SynBio Assembly Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic biology... -
Synthetic Biology - Fungal
TypeCollection...SynBio Assembly Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic biology... -
Synthetic Biology - Mammalian
TypeCollection...SynBio Assembly Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic biology... -
Synthetic Biology - Plant
TypeCollection...SynBio Assembly Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic biology... -
Synthetic Biology - Worm
TypeCollection...SynBio Assembly Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic biology... -
Synthetic Biology - Yeast
TypeCollection...SynBio Assembly Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic biology...