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We narrowed to 471 results for: FLU;

Showing: 101 - 120 of 471 results
  1. Luciferase Plasmid Collection

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    Collection
    ...donor and HaloTag labeled with a fluorophore as the acceptor. LumiFluors: EGFP-NanoLuc ( GpNLuc ) and LSSmOrange-NanoLuc...Nanoluc®. CalFlux VTN : An intracellular calcium sensor created by fusing NanoLuc® and Venus fluorophore to ...Constructs Other Highlights You may also like... Fluorescent Proteins Blog: Luciferase 101 Blog: Technologies...Addgene's collection include: Firefly luciferase (Fluc) Renilla luciferase (Rluc) Gaussia luciferase (Gluc...luciferase expression Stephen Tapscott 83281 pAAV-CAG-FLuc Firefly CAG AAV expression of firefly luciferase...luciferase Mark Kay 105532 pAAV.CMV.ffLuciferase.SV40 Firefly CMV AAV expression of firefly luciferase James Wilson... Wilson 101156 T7-CMVtrans-FFLuc-polyA Firefly T7 Expression of firefly luciferase from a cell-free mammalian...
  2. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

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    Blog Post
    ...lab fused a fluorescent protein to the hybrid anti-HA scFvs. The addition of the fluorescent protein allows...to amplify fluorescent signals in in vivo imaging: the 10xHA tag increased the fluorescent signal to noise...protein turnover Download the fluorescent protein eBook Browse our fluorescent protein blog posts Resources...Read our fluorescent protein guide Find plasmids for subcellular localization Find fluorescent protein ... part epitope binding region, all fused to a fluorescent protein. “Frankenbody,” like the infamous frankenstein...scFvs) (Inbar et al., 1972) are fused to mature fluorophores, and can bind and light up epitope tagged proteins...as living neurons, and can be fused to other fluorescent proteins and tags including mCherry, HaloTag ...
  3. New and Upcoming Viral Vectors - Spring 2019

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    Blog Post
    ...cre-dependent version at Addgene! Fluorescent protein and control AAV These fluorescent protein expression vectors...exhibits high contrast between peak fluorescence and resting fluorescence. It is useful for activity imaging...exhibits high contrast between peak fluorescence and resting fluorescence.  It is useful for activity imaging...pGP-AAV-syn-FLEX-jGCaMP7b-WPRE) exhibits the brightest resting fluorescence of the GCaMP7 family of calcium sensors and ...the endoplasmic reticulum, but exhibits larger fluorescent responses to GABA. Find the iGABASnFR and its...the control vectors for experiments encoding a fluorescent-tagged molecular tool.   Find pAAV-FLEX-tdTomato...of densely labeled neurons because background fluorescence from inactive neurons is reduced. 105322 ...
  4. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

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    ...DNA Fluorescence dyes Another way to quantify DNA would be to use fluorescent dyes that fluoresce when...and their corresponding fluorescence. That way, you can compare the fluorescence of your sample against...sequencing. Unlike absorbance-based methods, fluorescence-based methods require a standard curve, a set... have to do the calculation yourself as many fluorometers will calculate your sample concentration for... and you don’t need a spectrophotometer or a fluorometer to quantify DNA this way. First, start by pouring... run, DNA fragments move through a micro- or nanofluidic channel and the sample is separated by electrophoresis...These fragments are quantified over time using a fluorescent dye that intercalates into the DNA such that ...
  5. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

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    Blog Post
    ...protein (the FAP - fluorogen activating protein - dL5** in this case), and 2) a fluorogen (the TAP - targeted...photosensitizer - MG-2I in this case). The fluorogen is not fluorescent until it binds its protein partner thus...Up to Receive the Fluorescent Protein eBook Learn about Small Molecule Fluorophores Resources on Addgene.org...the protein complex complex and the MG-2I-dL5** fluorogen that can be used to ablate cells in culture and...It’s job is to bind MG-2I, a photosensitizer fluorogenic dye.  MG-2I is the TAP portion of the complex...which suggests that it could be used with these fluorescent proteins. He et al also compared MG-2I-dL5** ...head-to-head with KillerRed, a genetically encoded red fluorescent photosensitizer protein that generates ROS when...
  6. Antibodies 101: Buffers, Storage, and Conjugates

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    ...panel.  First, fluorophores vary in brightness. You don’t want a super-bright fluorophore, like PE, on ...assay you can do. Most non-HRP conjugates are fluorophores, which work not by reacting to a chemical, but...then read by a detector panel in the machine. Fluorophores are light sensitive, so antibodies conjugated...before you make your purchase. Picking a single fluorophore for an experiment is easy, but what about those...of interest! Research the brightness of your fluorophores before buying and try to inversely pair brightness...possible.      Fig 2: Try to inversely pair fluorophore strength with protein abundance.    Second...Second, you’ll need to consider spectral overlap. Fluorophores signal are actually quite broad, resulting in...
  7. Viral Vectors 101: Calcium Sensors

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    Blog Post
    .... This sensor switches from green fluorescence to a red fluorescent state only by simultaneous (1) user-controlled...Genetically encoded calcium indicators (GECIs) are fluorescent proteins linked to calcium binding proteins (... (e.g., calmodulin or troponin C). Their fluorescent readouts are used to monitor calcium concentrations... of the sensor, and (3) subsequent change in fluorescence only occurs at high calcium concentrations. ...reading with other measurements, specifically fluorescent ones? Many of the available biosensors use a ... methods Many calcium reporters use a single fluorescent protein (FP), requiring only a simple measurement...Structure-and mechanism-guided design of single fluorescent protein-based biosensors. Nature chemical biology...
  8. Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)

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    Blog Post
    ...of two fluorescent proteins with very specific spectral properties that limit which fluorophores can be...genetically encoded fluorescent kinase reporters. A KTR is comprised of a fluorescently-tagged substrate ...flexible swapping of fluorescent proteins and multiplexing is simple when fluorescent proteins with limited...The relative ratio of cytoplasmic vs. nuclear fluorescence serves as a proxy for kinase activity.  Advantages...cytoplasm, nuclear export and import rates can influence KTR translocation. Work from the Covert lab suggests...about photoswitchable kinases Learn more about fluorescent biosensors Learn about using phosphoserine to...Resources on Addgene.org Find FRET plasmids Find fluorescent biosensors ...
  9. Botman-Teusink Yeast FP Collection

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    Collection
    ...overexpress various fluorescent markers and tag your proteins of interest with fluorescent proteins in yeast...Teusink Lab Materials Kurt Thorn Lab Materials Fluorescent Protein Plasmids & Resources FP Tagging FP Overexpression...comprehensive collection of yeast codon-optimized fluorescent proteins (FPs) in a variety of plasmids allows...proteins of interest with yeast codon-optimized fluorescent proteins. These vectors are based on the pFA6a-link...Teusink Lab Materials Kurt Thorn Lab Materials Fluorescent Protein Plasmids & Resources External Resources...Goedhart J, Teusink B. In vivo characterisation of fluorescent proteins in budding yeast. Sci Rep. 2019 Feb ... WA, Thorn KS. Improved blue, green, and red fluorescent protein tagging vectors for S. cerevisiae. PLoS...
  10. Antibodies 101: Introduction to Antibodies

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    Blog Post
    ... be visualized using fluorophore-conjugated antibodies (i.e., immunofluorescence), or by a colorimetric... antibody, you can visualize the protein via fluorescence or chemiluminescence, precipitate the protein...protein to be detected by chemiluminescence or fluorescence. Western blots are frequently used to compare... primary antibodies that are conjugated to a fluorophore, to avoid the use of secondary antibodies in ... a mixture of cells. The technique of using fluorescently-labeled antibodies to detect proteins is referred...antibodies to bind to intracellular proteins. The fluorescence signal is detected by the flow cytometer and.... Because the signal comes from the antibody-fluorophore conjugate, and is not expressed by the cell itself...
  11. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

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    ...about other Fluorescent Biosensors A Practical Approach to Choosing the B(right)est Fluorescent Protein Visit...Visit the Fluorescent Protein Featured Topic Page Resources at Addgene.org Find Fluorescent Protein Biosensors...conformational changes lead to an increase in fluorescence intensity from the inserted GFP. After characterizing...expression levels in bacteria and produces stronger fluorescent signals upon sensing glutamate in mouse and ferret...higher expression levels and produce stronger fluorescent signals upon sensing glutamate using both 1-photon... Biosensors Browse All Fluorescent Proteins Find AAV Tools References Marvin, Jonathan S., et al. “An...An optimized fluorescent probe for visualizing glutamate neurotransmission.” Nature Methods (2013) PubMed...
  12. Viral Vectors 101: Biosensors

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    Blog Post
    ...generate a fluorescent output, which will turn on, change color, or change intensity of fluorescence in the...Epigenetics with a FRET Based Biosensor Fluorescent Proteins 101: Fluorescent Biosensors ...and the receptor, but readouts could involve fluorescence, pH change, heat, etc. The last component of...readout for whatever the bio-recognition event is (fluorescence, pH, etc.). The transducer will generate a numerical...   Neurotransmitters Many canonical fluorescent as well as FRET-based biosensors have been engineered...the ADP:ATP ratio. Most of these sensors are fluorescence-based and have been optimized for a wide-range...A. V., Shcherbakova D. M., Verkhusha, V. V., Fluorescent Biosensors for Neurotransmission and Neuromodulation...
  13. Visualizing Protein Turnover In Situ

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    ...observed with fluorescence microscopy. This application of AHA labeling is known as fluorescence noncanonical...localization of protein synthesis. Alternatively, fluorescent protein tags can be used to facilitate the visualization...protein synthesis in situ is the knock-in of fluorescent tags into the gene locus of interest. An advantage...there is the question of whether or not the tags influence behavior. A conference and a breakthrough! During...covalently links the azido containing AHA to a fluorescent alkyne tag. The newly synthesized and tagged ...amplification. The amplified DNA segment is labeled with fluorescent probes that enable visualization of the location...PMC4121734. Additional Resources on the Addgene Blog Fluorescent microscopy techniques - Which is best for me?...
  14. Seeing Red: Simple GFP Photoconversion

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    ...Addgene’s Fluorescent Protein Plasmids and Resources Relevant Blog Posts Plasmids 101: Green Fluorescent Protein...localization studies or make a reporter construct. Fluorescent proteins (FPs) are also the foundation of multiple...use in biological research. Photoconvertible fluorescent proteins Newly developed photoconvertible FPs...Sattarzadeh et al., as well as a wide variety of other fluorescent proteins for your imaging needs. If you need ...LL. A bright and photostable photoconvertible fluorescent protein. Nat Methods. 2009 Feb 6(2):131-3.  PubMed...Verkhusha VV. A photoswitchable orange-to-far-red fluorescent protein, PSmOrange. Nat Methods. 2011 Jul 31;...Protein Which Fluorescent Protein Should I Use? ...
  15. Plasmids 101: Secondary Nanobody Toolbox

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    Blog Post
    ...nanobody conjugated with Alexa Fluor 488, Alexa Fluor 568, or Alexa Fluor 647 before application to the...Western blots. ELISAs. Immunofluorescence. What do all of these techniques have in common? They all typically... distance between the target antigen and the fluorescent label, which can reduce the potential image resolution... resolution of super-resolution fluorescence microscopy. Their size also limits their ability to penetrate... This process can be avoided direct antibody fluorophore conjugates are used, but these are usually more...penetration and decreases the distance between a fluorescent tag and the target antigen to about 2 nm, which...secondary nanobodies conjugated with the desired fluorophores. In figure 4B below, triple colocalization is...
  16. New Norepinephrine Indicators: nLightG and nLightR

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    ...the Addgene blog Fluorescent proteins 101: Fluorescent Biosensors Which Fluorescent Microscopy Technique...circularly permuted green fluorescent protein (GFP), and nLightR uses a red fluorescent protein, mApple. Kagiampaki...findings that red fluorescent proteins tend to be less sensitive than green fluorescent proteins. nLightR..., J., & Li, Y. (2019). A Genetically Encoded Fluorescent Sensor for Rapid and Specific In Vivo Detection...Plasmids Find Optogenetics Plasmids Visit the Fluorescent Protein Guide Pages ...
  17. Sensing Neuronal Dopamine

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    ...has been in vivo microdialysis of extracellular fluid (Darvesh et al., 2011). This technique is performed...the semipermeable membrane from the extracellular fluid into the perfused liquid. The probe then collects...this, researchers from some labs have developed fluorescent-protein based DA sensors (Figure 2). The dLight1... sensors, the new dLight1 sensor is a single fluorescent protein-based sensor. This sensor was engineered...a GFP variant and a DA receptor such that GFP fluoresces when the receptor is bound by DA. Two variants...affinity and high-magnitude responses (increases in fluorescence) at low concentrations (e.g., 100 nm) of DA....variant bound to a DA receptor, such that the GFP fluoresces upon DA-receptor binding (Sun et al., 2018). ...
  18. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

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    ... of fluorescent proteins is only possible at low copy number ranges, resulting in low fluorescent protein... Cre-loxP system to express one of the three fluorescent protein (XFP) genes in a transgene. When multiple...Sakaguchi et al., 2018) (Figure 1). Once introduced, fluorescent proteins were best imaged after tissue clearing... expression levels. Clearing large tissues - Fluorescent proteins are very stable in SeeDB2. However, ...matching of tissue samples for High-resolution fluorescence imaging using SeeDB2." Bio-Protocol 8 (2018)...Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system." Nature 450.7166...multicolor labeling of neuronal circuits with fluorescent proteins and chemical tags." eLife 7 (2018): ...
  19. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

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    ...stranded DNA bound by tTALE-FP with the fluorescent protein fluorescing green. Image from Shin et al., 2019...intercalating fluorescent dyes for DNA-protein interaction studies? Kyubong Jo's lab generated fluorescent protein...this vector is suitable for flow cytometry and fluorescence microscopy, they also show that this method ...dx.doi.org/10.15698%2Fmic2020.04.713 Truncated TALE-fluorescent protein fusions as a DNA staining dye Article...specific DNA sequences which brings the attached fluorescent protein to the DNA. They created plasmids for...tTALE-mCherry. In comparison to intercalating fluorescent dyes which can cause photo-induced DNA breakage... lab also used tTALE-FP in a single molecule-fluorescence assay to monitor restriction enzyme digestion...
  20. 10 Great Guest Posts We're Thankful For

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    ...work!   Fluorophores When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein...us that, although fluorescent proteins are incredibly useful, chemical fluorophores can still be used ...Better Dyeing Through Chemistry & Small Molecule Fluorophores In this post, Luke Lavis from Janelia Research...variety of experiments. Read more to find chemical fluorophores for your next experiment. Nucleic acid based...to different pH’s and some that are fused to fluorescent proteins. She also showed that R-bodies can rupture...to achieve more efficient genome editing - all influenced by an understanding of Cas9 biophysics! Casilio...genes in a single cell, and localization of a fluorescent signal to your target gene. Read this post to...
Showing: 101 - 120 of 471 results