We narrowed to 952 results for: cha;

...MSCV Retroviral construct for miRNA expression. Chang-Zheng Chen 52114 pMSCV-IRES-mCherry FP MSCV A bicistronic...

...activity of three-fold or greater in a well-characterized bacterial two-hybrid reporter system but have...

... One-step assembly of antibody heavy- and light-chain DNAs into a single mammalian expression vector A...

... flap is excised, the target sequence remains unchanged and available as a substrate for another round...

... AAV retro helper plasmid from the Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . ...

...Help Center Check out Addgene’s Help Center, a searchable database of information to help you every step...

...-dependent expression of the voltage-gated Na+ channel mNaChBac and (physically separate) tdTomato. 8 ...

...diseases, are listed below: Lipid droplet reporters - Richard White Lab. An imaging platform to study lipid dynamics...

... AAV retro helper plasmid from the Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . ...

...and Myers Tagging System The Eric Mendenhall and Richard Myers labs have deposited plasmids for a CRISPR-based...

...supports a wide range of organims. Developed by the Michael Boutros Lab . CasFinder: Flexible algorithm for...

...Fluorescent Protein (FP) Guide and the Michael Davidson FP Collection NLS Nuclear localization...

...references are below. The Cas9 knockin mouse can be purchased from Jackson Labs: Cre-dependent Cas9 mouse Constitutively...

...1–10 µL single channel pipette 2–20 µL single channel pipette 20–200 µL single channel pipette 200–1000...Insert tab, then Chart . This will add a blank chart to your spreadsheet. In the Chart Editor , select ...select the Chart Type drop down menu and select Scatter chart. Change the data range and select the cells where...1000 µL single channel pipette Pipette controller Microcentrifuge Electrophoresis chamber Power supply ...running buffer. Fill the chamber with 1X MOPS running buffer. Make sure that the chamber is sealed. Load 5 µL...and unplug the electrophoresis chamber. Remove the gel from the chamber. Use the metal spatula to gently...and ~25 kDa corresponding to the heavy chain (HC) and light chain (LC) proteins, respectively. There should...

...1–10 µL single channel pipette 2–20 µL single channel pipette 20–200 µL single channel pipette 200–1000...1000 µL single channel pipette 20–200 µL multichannel pipette Multichannel pipette reagent reservoirs Pipette...determine when the desired color change has occurred. The color will change from yellow to blue and generally... desired color change has occurred, gently remove the plate seal. Using a multichannel pipette, add 100... and set aside. Aspirate the wells and use a multichannel pipette to add 200 µL wash buffer to each well...tube and invert several times to mix. Using a multichannel pipette, add 200 µL of blocking buffer to each...

...10 µL single channel pipette 20–200 µL single channel pipette 200–1000 µL single channel pipette 1–10 ...10 µL multichannel pipette 2–50 µL multichannel pipette 20–200 µL multichannel pipette Vortex, VWR, 10153...Use multichannel pipettes for the dilution series. For dilutions 1–5, use the 1–10 µL multichannel pipette...polystyrene reagent reservoir. Using a 20–200 µL multichannel pipette, carefully add the dilution buffer to...dilution scheme listed in step 13. Use a single channel 1–10 µL pipette to add 5 µL of each viral sample...slowly to avoid generating aerosols. Be sure to change tips each time. Dilution 1 (20X): 5 µL in 95 µL...to 5 µL. For dilutions 6–8, use the 20–200 µL multichannel pipette set to 50 µL. For mixing between dilutions...

...0.1–1 mL single channel pipette 0.5–10 µL single channel pipette 20–200 µL single channel pipette 2–20 µL...Option 2 with a buffer exchange/concentration using an Amicon 30 kDa MWCO buffer exchange column. (Optional... Day 1: Purify antibody Day 2 or later: Buffer exchange Equipment Class II, Type A2 Biological Safety ... µL single channel pipette Reagents Aspirating pipette, VWR 414004-265 5 mL pipette, VWR 89130-896 10 ...above 1.0 mg/mL proceed to Option 1 with a buffer exchange using a Zeba Spin 7 kDa MWCO desalting column....the same recombinant antibody. Section 2: Buffer exchange Choose Option 1 or Option 2 based on the concentration...concentration of the pooled sample above. Option 1: Buffer exchange using a desalting column The 10 mL Zeba Spin Desalting...

...10 µL single channel pipette 20–200 µL single channel pipette 200–1000 µL single channel pipette 1–10 ...10 µL multichannel pipette 2–50 µL multichannel pipette 20–200 µL multichannel pipette Reagents Universal...i.e master mix + water Whenever possible use a multichannel pipet to minimize pipetting error and variability...immediately before use. Use a reservoir and a multichannel pipette to dispense the master mix into the ...red were calculated using this plasmid, but will change if you use a different plasmid. Sample Calculation...with >50 uL if your well contains 100 uL) Use a multichannel pipette to load the standards and samples onto...

...10 µL single channel pipette 20–200 µL single channel pipette 200–1000 µL single channel pipette 2–50 ...50 µL multichannel pipette 20–200 µL multichannel pipette Ice bucket 96-well freezer blocks Vortex, VWR...appropriate amount of media volume listed in the chart below. For the first virus dilution, add 160 µL ...dilution. Repeat for all dilutions as indicated in the chart below. Dilution Factor Virus Volume to Dilute Media...cartridge into the cartridge holder. Using a 2–50 µL multichannel pipet, load 20 µL of the reaction mixtures into...polystyrene reagent reservoir. Using the 20–200 µL multichannel pipet, load 70 µL of droplet generation oil ...droplets have been generated, use a 20–200 µL multichannel pipette to collect 40 µL of droplets. Pro-Tip...

...0.5–10 µL single channel pipette 2–20 µL single channel pipette 20–200 µL single channel pipette 200–1000...fresh media containing selection reagent Day 3–14: Change media as needed Day 14–18: Expand and harvest stable...1000 µL single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container Reagents... one suitable with your protocols. FBS can be purchased already heat-inactivated, or it can be inactivated...lentivirus, above) are dying. Perform regular media changes and monitor the growth of the cells. Pro-Tips Depending...culture, so it is important to do regular media changes and maintain optimal growth conditions for the ...absence of cell death, the cell media should be changed every 2–3 days to maintain the dose of antibiotic...