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Showing: 101 - 110 of 110 results

  1. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...GAL4 gene downstream of a tissue- or developmental stage-specific promoter and design a UAS reporter construct...genes for metabolic engineering applications in Saccharomyces cerevisiae.” FEMS Yeast Res. 12(2) (2012): 197...

  2. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs...pyogenes URA3 Wyrick pCRISPomyces-2 61737 Bacteria BbsI yes, cut S. pyogenes Apramycin Zhao pRB1017 59936...none S. pyogenes Zon pCRISPomyces-1 61736 Bacteria BbsI yes, cut S. pyogenes Apramycin Zhao pMB70 47943 Worm...pyogenes tagRFP Ebert pL-CRISPR.SFFV.tRFP 57826 Mammalian/Lentiviral BsmBI yes, cut S. pyogenes tagRFP Ebert...activate, interfere, or nick. Selection , such as Puromycin or EGFP Cloning enzyme used for insertion of your...Mammalian/Lentiviral BbsI none S. pyogenes Puro, TagBFP Yusa lentiGuide-Puro 52963 Mammalian/Lentiviral...Mammalian/Lentiviral BsmBI yes, cut S. pyogenes tagRFP Ebert pFGC-pcoCas9 52256 Plant AscI, PacI, SbfI...

  3. Lentiviral Prep Service

    Type
    Collection
    ...carrying version 1 of the CellTag barcoding library. Version 1 of the Celltag library contains 19973 barcodes...carrying version 2 of the CellTag barcoding library. Version 2 of the Celltag library contains 4934 barcodes...carrying version 3 of the CellTag barcoding library. Version 3 of the Celltag library contains 5737 barcodes... genes along with 1000 non-targeting controls Puromycin Doench , Root 73179 Human gRNA pooled library ... genes along with 1000 non-targeting controls Puromycin Doench , Root Human activation pooled libraries...56,762 unique sgRNAs targeting 18,885 human genes Puromycin Doench , Root Human inactivation pooled libraries...human genes along with 500 non-targeting controls Puromycin Doench , Root 92386 Human CRISPR inhibition library...

  4. Lentivirus Plasmids

    Type
    Collection
    ... genome, it has the potential for insertional mutagensis. The 2nd and 3rd generation lentiviral systems...-pLKO-puro 3rd inducible expression of shRNA; puromycin selection. See plasmid 21916 for Neo selection...cDNA expression, gateway plasmid, constitutive Hygromycin resistance gene coexpression. See article for...19319 pLJM1-EGFP 3rd for EGFP fusion; PGK driven puromycin Sabatini 25895 pLX301 3rd Gateway plasmid for ...Tet-pLKO-neo 3rd inducible expression of shRNA; neomycin selection. See plasmid 21915 for Puro resistance...

  5. Lentiviral Guide

    Type
    Guide
    ... genomes have selectable markers, such as the puromycin resistance gene, conferring antibiotic resistance...the transfer vector during the viral production stage. Many of the lentiviral transfer vectors that have...Flap. Zennou V, Petit C, Guetard D, Nerhbass U, Montagnier L, Charneau P. Cell. 2000. 101(2): 173-185. PubMed...

  6. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...joining (NHEJ) repair. Deletions have potential advantages as compared to single-site small indels given...Addgene plasmid ID 48139), which include GFP and puromycin as selectable markers, respectively, or constructs...consists of DMEM supplemented with 2% penicillin/ streptomycin and 1% L-glutamine, which is used for MEL cells...

  7. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...Loren Looger AV-1-PV3661 98927-AAV1 pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 Control Loren Looger AV-10-PV0101...James M. Wilson AV-9-PV2453 45185-AAV9 AAV-EF1a-BbTagBY Control Joshua Sanes AV-9-PV2454 45186-AAV9 AAV-EF1a-BbChT...Boyden AV-1-PV3511 98926-AAV1 pAAV.CAG.GFPsm-myc.WPRE.SV40 Loren Looger AV-1-ALL856 100048-AAV1 pAAV.CAG.LSL.tdTomato...

  8. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...apoeb TAL3402 & TAL3403 TGCCAGGCTCGTAGCCTGttccaggctgatgccccTCAGCCCAGATGGGAGGA arap3a TAL3026 & TAL3027...ARSA TAL 3002 & TAL 3003 TCAAGAGGCGGTCTCCCTctcaacgagaccaccaTAGCCGAGGTGCTGAAGA asun TAL3404 & TAL3405 TAACTCAAGCCAACTCCAggaaacatcaggagtttGCAGGAAGATTGAGTTCA...cited2 TAL3228 & TAL3229 TGGTAGACCGCATGATGGcaatgaaccatggtcgTTTCCCAGATGCTGTGAA cited4a TAL3046 & TAL3047...clcn1b TAL3052 & TAL3053 TTTTATTGTGTTACAGACtgcctagcccgtgtccAGAGGTATATTGTGACAA col22A1 TAL3410 & TAL3411...crispld2 TAL3232 & TAL3233 TATCAGGAGGAGCTAGAAcccaacagcactaaacCCGACGCTCCTGTCCGAA cx52.6 TAL3056 & TAL3057...CYP20A1 TAL3414 & TAL3415 TAGACTTTCCTGAAAAGCaaaaatgctagattttGCCATTTTTGCTGTGACA dachc TAL3234 & TAL3235 ...TCCCGTGCAAGGAATGAAtgcaaaaacatttcctTTCCCTTGTTTTGACGGA dcps TAL3416 & TAL3417 TGAACAGTCCACCGATAAcccagtggtagacttcCAGCTCACCCGCATTCTA ddx26b TAL3418 & TAL3419...

  9. Retrovirus Plasmids

    Type
    Collection
    ...results in long-term transgene expression. Some disadvantages of γ-retroviruses are that they can only transduce... can lead to oncogenesis (termed insertional mutagenesis). Read our γ-Retrovirus Guide for more information... For cloning and gene expression; select with puromycin or screen for GFP Bartel 32702 pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE...

  10. Ras Pathway

    Type
    Collection
    ...protein, LST8 homolog MTOR Mechanistic target of rapamycin NF1 Neurofibromin 1 NFE2L2 Nuclear factor, erythroid...SPRY1 SPRY2 SPRY3 SPRY4 Sprouty RTK signaling antagonist STK STK3 STK4 Serine/threonine kinase STK11 Serine...
Showing: 101 - 110 of 110 results