Skip to main content
Addgene

We narrowed to 541 results for: SHI

Showing: 121 - 140 of 541 results
  1. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    ...like-minded science communicators? Attend the annual flagship meetings of AAAS, WCSJ or ECSJ. These meeting ...in the UK, join the SciComm mailing list. 7) Internships – Learn on the job Nothing prepares you better...better than learning on the job. Try to get an internship at a magazine or newspaper (here’s a partial list...). A great opportunity is the AAAS Mass Media Fellowship, which places science students for 10 busy weeks...American or National Geographic. Similarly, media fellowships from the British Science Association allow you...Society of Experimental Biology offers press internships to cover their annual meetings. 8) Training –...
  2. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...https://doi.org/10.1126/science.aat0839 Yoshimi, K., & Mashimo, T. (2022). Genome editing technology and...all-in-one Cascade plasmids — such as the Tomoji Mashimo lab and the Tilmann Weber lab — to increase efficiency...doi.org/10.1016/j.molcel.2023.01.024 Morisaka, H., Yoshimi, K., Okuzaki, Y., Gee, P., Kunihiro, Y., Sonpho...Yamamoto, T., Sano, S., Hotta, A., Takeda, J., & Mashimo, T. (2019). CRISPR-Cas3 induces broad and unidirectional...s41587-019-0310-0 Makarova, K. S., Wolf, Y. I., Alkhnbashi, O. S., Costa, F., Shah, S. A., Saunders, S. ...
  3. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    ...skills that create a full package and add an extra shine to your work. Knowing your transferrable skills ...using your charisma to give people hope for some shiny vision on the horizon. You’re about to keep silent...opportunity much different? Might you already have leadership skills that are just not being tapped at the ...this time to step up and develop your nascent leadership skills. You might just find that you’re more ...didn't even know you possessed. Think about your shining moments. Recall a few times you really felt great...Managing a team Conflict resolution Organization Leadership Problem Solving Creativity Data analysis Listening...
  4. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...contributed by Ryan Watkins, Professor at George Washington University and developer of wesharescience.org...English is a second or third language (making publishing their research all the more challenging even ...Hamilton, biology professors at the University of Washington in St. Louis, offer guidance on how to make a...Rhythm and Melatonin for the Timing of Nocturnal Courtship Vocalization. The video summarizes the research...science! Ryan Watkins is a professor at George Washington University and he the developer of the WeShareScience.org...journals: An overview and case study." Journal of Librarianship and Scholarly Communication 2.2 (2014): 2. Additional...
  5. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...pCAG-EGxxFP target plasmid and pX330-sgRNA plasmid (Mashiko et al. 2013). The pCAG-EGxxFP target plasmid contains...indicates a more successful gRNA design. Adapted from Mashiko et al., 2014. Not all gRNA’s are effective....fluorescent signal, for details on this plasmid, see Mashiko et al., 2013). Testing the efficacy of your gRNAs...validation assay (above) and it is just as effective (Mashiko et al., 2014). The plasmid is sturdier than the...24253446. PubMed Central PMCID: PMC3875854. 2. Mashiko, Daisuke, et al. "Generation of mutant mice by ...24284873. PubMed Central PMCID: PMC3842082. 3. Mashiko, Daisuke, et al. "Feasibility for a large scale...
  6. March for Science

    Type
    Blog Post
    ...formal stance taken by Addgene or its staff. In Washington, D.C. as well as sister locations on April 22...inherently political so why is there a march in Washington, D.C., the seat of the United States government...citizens. Thus civilization proceeds in an informed fashion. For science to be useful to governments, however... for Science has the details for the march in Washington as well as the satellite marches. Anyone can ...Climate Change References Purged from Website The Washington Post: Trump administration tells EPA to freeze...Line The New York Times: A Scientists’ March on Washington Is a Bad Idea Pew Research Center: Public Praises...
  7. Providing Plasmids to Researchers in Developing Countries: Addgene and Seeding Labs Team Up

    Type
    Blog Post
    ...facilitating connections to mentorship and networking opportunities, publishing assistance, and more to Instrumental...everywhere, but resources are not. Through its flagship program, Instrumental Access, Seeding Labs partners...research in the labs they’re granted to, as well as shine a light on some of the great minds working in developing...Castano Corsino’s Recent Post on the International Mentorship Program Learn How to Network and Meet People ...
  8. Introducing an All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering

    Type
    Blog Post
    ...Cas9 compared to ZFNs and TALENs,” said Tetsushi Sakuma of Hiroshima University. “However, there had been...now available thanks to researchers at Japan’s Hiroshima University who described their new tool in Scientific...they’ve filled that gap. As Sakuma and colleague Takashi Yamamoto describe in their recent study, the construction...all-in-one CRISPR/Cas9 vector system. Sakuma T, Nishikawa A, Kume S, Chayama K, Yamamoto T. Sci Rep. 2014...
  9. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ...speakers from academia, industry, nonprofits, and publishing. The livestream video from the event can be found...should be reproducible. Indeed, researchers are pushing the limits of knowledge as they develop new ways...single, searchable space. Finally there are new publishing outlets like F1000, PeerJ, PLoS, and bioRxiv ...adoption of policies that promote reproducible publishing practices. One example is the publication of ...Within universities, we’ll also hopefully see a shift toward hiring and promotion practices that focus... discussion to learn a bit more about how the publishing community views reproducibility, how industry...
  10. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    ...’t until much later when, in the 1960s, Osamu Shimomura studied the bioluminescent properties observed...green light. To investigate this green glimmer, Shimomura collected many, many jellyfish specimens from ...Sound in the Pacific Ocean, off of the coast of Washington state. Using these samples, he was able to isolate...mutations that alter and improve its function and shift its emission spectrum. New fluorescent proteins ...when the Nobel Prize in chemistry was awarded to Shimomura, Chalfie, and Tsien for their work on GFP. This...
  11. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...conferences. The Barry Goldwater scholarship is also a national scholarship that supports students interested...t know yet has helped me cultivate stronger relationships with my labmates. Sometimes what I thought was...makes all the difference in a lab experience. “Mentorship” was a bit of a foreign concept to me when I ...online for a while and observed. Apply for those scholarships that you don’t think you’re qualified for. Wayne...populations. Research universities often have scholarships and funds dedicated for undergraduates interested...
  12. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ... distribution of research materials. Our small shipping and handling fees drive our work. As we have grown...confidentiality where it is appropriate. This grows our relationship with our community members. We also find that...that, as a nonprofit, we attract partnerships with organizations that share our community-oriented values...Michael Lo., Michael Le., Michel, Michelle, Morgan, Natashia, Nicole, Olivia, Pamela, Racheal, Ramsey, Randall...Learn More About Our Mission Meet the Addgene Leadership Team Get the Latest News from Addgene ...
  13. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ...scientific publishing world and debate issues about how to improve the scientific publishing process. There...as financially critical. Those who work on the publishing side of science have professional meetings, societies...Kitchen’ for a glimpse into the world of scholarly publishing. And see for example the Eighth International...science communications world, you might consider an internship or a short program to build skills and connections...
  14. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    ...We are excited about our new partnership with Allele Biotechnology which allows researchers to deposit...Because the excitation and emission maxima were red-shifted (462 nm and 492 nm, respectively), they gave this...further sought to identify why this protein was blue-shifted compared to its parent protein CFP484. Collaborating... that specific amino acid changes favored blue-shifting more than others. During this process, they identified...Central PMCID: PMC1698604. Ai, Hui-wang, et al. "Hue-shifted monomeric variants of Clavularia cyan fluorescent...
  15. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    ...from Kyoto University, and Marcus N. Leiwe and Takeshi Imai from Kyushu University. Stochastic multicolor...from Kyoto University, and Marcus N. Leiwe and Takeshi Imai from Kyushu University. Richi Sakaguchi is...anatomical maturation of the olfactory system.      Takeshi Imai is a professor at Kyushu University. His lab...2732. PubMed PMID: 26972009. Ke, Meng-Tsen, and Takeshi Imai. "Optical clearing and index matching of tissue...PMC4246963. Sakaguchi, Richi, Marcus N. Leiwe, and Takeshi Imai. "Bright multicolor labeling of neuronal circuits...
  16. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ... 181-206. PubMed PMID: 17224625. Mizushima, Noboru, Tamotsu Yoshimori, and Beth Levine. "Methods in mammalian...PubMed Central PMCID: PMC4517063. Yoshii, Saori R., and Noboru Mizushima. "Monitoring and measuring autophagy...20144757. PubMedCentral PMCID: PMC2852113. Noda, Takeshi, and Daniel J. Klionsky. "The quantitative Pho8Δ60...
  17. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...010822 Nishimasu, H., Ran, F. A., Hsu, P. D., Konermann, S., Shehata, S. I., Dohmae, N., Ishitani, R., ..., Isomura, K., Kajimoto, S., Komori, T., Nishimasu, H., Shima, T., Nureki, O., & Uemura, S. (2018). Real‐time...they are completely unique to the CRISPR system (Nishimasu et al., 2014). Table 1. Summary of Cas9 domains... the last base pair with an end-capping loop (Nishimasu et al., 2014). This cap is where the DNA:RNA heteroduplex...
  18. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ...framework If you can’t find internships at science companies, try to find internships with translatable skills...career: Try to get software product management internships, preferably on teams that run within the agile...project management  Bolster your skills in strong relationship-building and influence. You’ll need a large ...large deal of influence and strong relationship-building in order to rally many stakeholders behind a common...
  19. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    ...no better place for a scientist to make new relationships than at scientific conferences. Conferences ...this is a great ice breaker. Of course, real relationships go further and grow over time, but being interested...fun, memorable meal is a great way to build relationships. Make a list of experimental problems or questions...work – casual socializing is how people build relationships that are memorable. It isn’t always appropriate...Thesis Whisperer "Not Networking - Building Relationships for Success" webinar with Joanne Kamens via ...
  20. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    ...participants in skills useful for future mentoring relationships.   If you would like to start a group mentoring...for the time commitment of a formal mentoring relationship or there aren’t enough senior mentors available... the discussion. This is a chance to practice leadership and communication skills. The leader also provides...ideas on what to do once you are in a mentoring relationship.  Celebrate your successes and support each... come. Are you involved in Peer Mentoring relationships? Comment below with ideas from your own mentoring...
Showing: 121 - 140 of 541 results