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We narrowed to 273 results for: aav

Showing: 181 - 210 of 273 results

  1. Chemogenetics Plasmids

    Type
    Collection
    ...Chemogenetics Guide Viral Service: Chemogenetics AAV Blog: Chemogenetics Viral Vectors 101 Blog: Chemogenetics...collection. For example, search for "hM4D mCherry", "KORD AAV", "LMO", "Sternson", or other terms. Looking for ...for viral preps? We also offer ready-to-use AAV preparations of many chemogenetics plasmids. ID Plasmid ...

  5. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...about how to minimize leakiness in experiments using AAV vectors. Highlighted Plasmids Browse the tables below...preps feature Cre-dependent tools, or consider our AAV Packaged on Request service for more options. Addgene...Activity with GFP Plasmids 101: FLEx Vectors Optimizing AAV DIO and FLEx Vector Expression Plasmids 101: Knockout...preps feature Flp-dependent tools, or consider our AAV Packaged on Request service for more options. Addgene...CRISPR Knockout Library CRISPR Sidi Chen Use this AAV CRISPR knockout library expressing luciferase and...Return to top Related Addgene Plasmid Collections AAV Enhancer Collection Fluorescent Biosensors Chemogenetics...

  6. Tetracycline Inducible Expression

    Type
    Collection
    ...APEX targeting Alice Ting 85040 pK170.AAV-TRE-Cre-WPRE (Supernova) AAV vector for dox-inducible expression...sgRNA TetR H1-2O2 Nathanael Gray 35625 pAAV-Ptet-RFP-shR-rtTA AAV Tet-On shRNA vector. To evaluate shRNA...promoter Tet-On 3G rtTA David Vereide 120309 pAAV-FAH-rtTA3G AAV vector to express Tet-On 3G transactivator...promoter TetR Eric Campeau , Paul Kaufman 175274 pAAV-rtTA AAV Tet-On vector with neuron-specific expression...Plasmid #27106 for rtTA. tTA Edward Hsiao 99118 pAAV-CAG-tTA AAV Tet-Off vector, expresses tTA from the CAG... Tet-Off tTA-Advanced Takeshi Imai 104109 pAAV-Syn1-tTA AAV Tet-Off vector, expressed tTA from the hSyn...transactivators and inducible tools or consider our AAV Packaged on Request service available for many transactivator...

  7. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    ...transgenic DNA exceeding the carrying capacity of AAV vectors." Gene Therapy for Neurological Disorders...Hudry, Eloise, and Luk H. Vandenberghe. "Therapeutic AAV gene transfer to the nervous system: a clinical reality...Maguire, 2016). Recombinant adeno-associated viruses (rAAVs) have become increasingly popular for gene delivery...immune responses (Fenno et al., 2011). However, rAAVs lack the ability to specifically infect defined ... promoters, on the other side, are too long for rAAVs, which can only package sequences shorter than 5...vectors in combination with transgenic animals or rAAVs expressing Cre under a specific cell-type promoter... 2019). For example, Taylor and colleagues used rAAV FLEx-based vectors to study the neural circuitry...

  8. Luciferase Plasmid Collection

    Type
    Collection
    ...Schulze-Lefert 83282 pAAV-CAG-RLuc Renilla CAG AAV expression of Renilla luciferase Mark Kay 83280 pscAAV-CAG-RLuc...Description PI 60226 AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Renilla EF1α AAV-CRISPR knockout...William Hahn, David Root 105533 pAAV.CMV.Luc.IRES.EGFP.SV40 Firefly CMV AAV expression of firefly luciferase...luciferase expression Stephen Tapscott 83281 pAAV-CAG-FLuc Firefly CAG AAV expression of firefly luciferase Mark...Mark Kay 105532 pAAV.CMV.ffLuciferase.SV40 Firefly CMV AAV expression of firefly luciferase James Wilson...expressing luciferase. We also offer ready-to-use AAV preparations of select luciferase expression plasmids...Renilla CAG Vector for packaging self-complementary AAV expressing Renilla luciferase Mark Kay 140328 pLenti-PGK-Venus-Fluc...

  9. What the HEK?

    Type
    Blog Post
    ...Troubleshooting Additional resources on Addgene.org AAV Production in HEK-293T Cells  ...

  10. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...of GCaMP8 AAV preps has expanded. Check out the new selection of ready-to-use AAV9 and AAVrg vectors. ...available in AAV8! Expand your imaging experiments with the Cre-OFF/FLP-ON vector 137128-AAV8: Ef1a-Coff...

  12. Chemogenetics Guide

    Type
    Guide
    ...they drive expression. Different AAV serotypes can be used to target AAV vectors to specific cells of interest...considered. For tissue specificity of AAV serotypes, see our AAV guide . FLEx Vectors are also used to...models. For viral injection, Adeno-associated virus (AAV) is a widely-used tool for achieving in vivo expression...of chemogenetic receptors, and a wide variety of AAV-encoding chemogenetics plasmids are available. Additionally...available. Browse our collection of Ready-to-use AAV Preparations of Chemogenetic Plasmids ! Table 4. ...Targeted expression. Depending on the experiment, an AAV-encoded chemogenetic plasmid may need to be targeted...regions of a neuron. Cell-specific expression of AAV-delivered constructs can be controlled with cell-...

  17. Plan Your Experiment

    Type
    Guide
    ... collection of in-stock lentiviral preps . AAV vectors AAV vectors offer some advantages for viral vector...delivery. Both lentiviral and AAV vectors are safe to use in the lab, but AAVs exhibits the lowest immunogenicity... transgene expression with AAVs can be long-lived, despite recombinant AAVs not integrating into the host...also frequently use AAVs to develop CRISPR-based therapeutics. One limitation of AAV vectors is that the...blog post on overcoming the AAV size limitation . Browse Addgene's in-stock AAV preps or try Addgene's Packaged...delivery, lentiviral and adeno-associated virus (AAV) vectors are the most common. Using viral vectors...vector) is smaller than that of lentivirus. Thus, AAVs are generally only compatible with smaller Cas enzymes...

  18. Neurodegeneration Research Collection

    Type
    Collection
    ... Nat Biotechnol. 2023 May 22. See More AAV Viral Preps Find AAV viral preps for systemic delivery of viral...research. Disease Info Plasmid Collection CRISPR Tools AAV Viral Preps iPSC Differentiation Factors Antibodies...Oct 18. Target neural oxytocin receptors using an AAV-CRISPR/Cas9 strategy for gene editing across divergent...brain cells and more. New and Noteworthy: AAV.rTH.PI.Cre.SV40 ready-to-use viral prep useful for dopamine...

  19. Optogenetics Plasmid Collection

    Type
    Collection
    ...Collection You may also like... Optogenetics Guide AAV Viral Service Biosensors Chemogenetics Plasmids Chemogenetics...Looking for viral preps? We also offer ready-to-use AAV preparations of many optogenetics plasmids. ID Plasmid...

  20. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...and to compare different Cas enzymes in all-in-one AAV delivery. While Fragmid developers were specifically...Fragmid toolkit to facilitate other lentivirus- and AAV-based studies. Unified MoClo Systems While many MoClo...

  23. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...pX601 61591 Mammalian/AAV BsaI yes, cut S. aureus Zhang pX602 61593 Mammalian/AAV BsaI yes, cut S. aureus...pyogenes Joung AAV:ITR-U6-sgRNA (Backbone) PCB-FlPO-WPRE-syntetisk pA-UTR 68347 Mammalian/AAV none S. pyogenes...pyogenes mCherry Kuhn AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR 60229 Mammalian/AAV SapI none S. pyogenes...pyogenes Zhang AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR 60231 Mammalian/AAV SapI none...pyogenes Zhang AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR 60226 Mammalian/AAV SapI none S...particular expression system , eg. Mammalian, Lentiviral, AAV, Bacteria, Drosophila, Yeast, Plant, Xenopus, Worm... none S. pyogenes Gersbach PX552 60958 Mammalian/AAV SapI none S. pyogenes Zhang pgRNA-humanized 44248...

  24. Hot Plasmids - February 2022

    Type
    Blog Post
    ...off-target effects, yet small enough to be packaged in AAV. A dCasMini-mediated adenine base editor allows for...

  26. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...been deposited to Addgene, including lentiviral and AAV expression vectors for certain variants. Klapoetke... infeasible. Finally, an adeno-associated viral (AAV) system enables greater spatio-temporal control over... either the Thy1 or CAG promoter, while Brainbow AAV is under control of the EF1a promoter. Livet et...

  27. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ... 74307 pAAV-CMV-DIO-Magneto2.0-sNRPpA AAV 74306 pAAV-CMV-DIO-TRPV4-p2A-ferritin-sNRPpA AAV 74302 ...plasmids, which already includes dSaCas9-VPR in an AAV transfer vector for in-vivo expression and plasmids...Magneto2.0 in the mammalian brain after delivering it via AAV injection, finding that it could increase the activity...lines by inserting OsTIR1 into the "safe harbor" AAVS1 locus (a tet-inducible OsTIR1 plasmid is also available...

  28. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...protein production AAV-GfaABC1D-MCS--4x6T-WPRE - For astrocyte-selective expression in AAV Dual promoter Separate...Advantages Representative Empty Backbones AAV High transduction efficiency, but do NOT integrate...expression, see our dedicated Adeno-associated Virus (AAV) Plasmids page Lentiviral Can transduce both dividing...

  30. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...it easier to package for therapeutic delivery by AAV, which has strict capacity limitations. New efforts...Science, 362(6416): 839-842 (2018). 10.1126/science.aav4294 Ozcan, Ahsen, Krajeski, Rohan, Ioannidi, Eleonara...
Showing: 181 - 210 of 273 results