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We narrowed to 642 results for: ato

Showing: 181 - 210 of 642 results

  1. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    ...ways scientists have adapted them for use in the laboratory. The discovery of GFP The story of how fluorescent...sequence. Soon after that, in 1994, Prasher’s collaborator Martin Chalfie expressed GFP in exogenous organisms...studying it grew. In particular, studies in the laboratory of UCSD biochemist Roger Tsien resulted in much...

  3. Chemogenetics Plasmids

    Type
    Collection
    ...Drugs (DREADDs) and Pharmacologically Selective Actuator Modules (PSAMs). DREADDs are the most widely used...optogenetic element, making a bimodal opto-chemogenetic actuator. When the luciferase enzyme oxidizes its substrate...

  4. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...Find CRISPR plasmids to study the regulatory networks involved in retinal development....mouse retina. To aide in their studies of the regulatory networks involved in retinal development the ...

  5. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    ...transduction of Cas9-expressing (Rosa26-Cas9) hematopoietic stem cells with a lentiviral vector containing...LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard Medical School and is interested in...G., & Sharpe, A. H. (2024). X-CHIME enables combinatorial, inducible, lineage-specific and sequential ...

  6. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    ...Vampire bacteria Vampirovibrio chlorellavorus is a predatory bacteria that attaches to the cell wall of green...forcing it out of hiding to become exposed to predators. Hungry birds spots the “caterpillar” and then... "Back from the dead; the curious tale of the predatory cyanobacterium Vampirovibrio chlorellavorus." ...

  7. Recombinases AAV Preps

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    Collection
    ... mCherry 1 Yang 107738 pAAV-hSyn-Cre-P2A-dTomato Syn dTomato 1, 2, 5, 8, 9, rg*, PHPeB Larsen 107787 AAV.TBG.PI.Cre.rBGe...

  8. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    ... interpret signals from these reporters. Lack anatomical context/spatial localization: In vivo imaging...years to clinically treat enteric infection and inflammatory bowel conditions. In these experiments, arg1...abdomen. arg1 signal, however, can be overlaid onto anatomical ultrasound imaging to show where the bacteria...

  9. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

    Type
    Blog Post
    ...department.” Caitlin does this by acting as a facilitator between her team, the sales department, and operations...business.” Caitlin’s advice for budding science communicators: “My recommendation would be to take any opportunity...education tools? Check out this Addgene podcast with educator and science rap mastermind Tom McFadden Get tips...

  10. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    ...scientists. Over the course of the class, the moderator discussed individual sections of a paper, the ...quizzes and discussion on that week’s topic by the moderators. The assignments, which consisted of reading ...course was breaking up into focus groups with a facilitator. In the focus groups, we were required to critique...

  11. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

    Type
    Blog Post
    ...passion, and dedication to sharing epitomized by the creators of these plasmids with the first annual Blue Flame...has “literally enabled sharing with 100's of investigators worldwide. Now everything from every paper is...Flame awardee Sophien Kamoun of the Sainsbury Laboratory in Norwich, UK: “We’re very pleased to distribute...

  12. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...plasmid. Parts shown in grey are optional cis-regulatory elements.   Key elements of an AAV plasmid...plasmid harbors non-coding sequences. Other cis-regulatory elements Next let’s talk about some of the elements...Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) forms a tertiary structure when...

  13. RUBY-Red Siliques

    Type
    Blog Post
    ...Willoughby   Using RUBY as a selection marker The creators of RUBY highlight its use in crop plants under... is difficult for new members of the lab or collaborators unfamiliar with these mutant plants. Using RUBY...promoter with self-cleaving 2A site linkages and a terminator. Image from He, Y., Zhang, T., Sun, H. et al....

  14. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...Neurobiology has been working on genetically encoded indicators of calcium and other small molecules since the...imaging for beginners Griesbeck says his calcium indicators can be used in mouse, Drosophila, C. elegans,...his team is now applying it to improve upon an indicator they developed and published in 2010 for visualizing...

  15. Distribution to Industry

    Type
    Collection
    ...signed by an authorized signatory at the recipient institution. An authorized signatory is someone who can ...

  16. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    ...15-30 minute break. The method is named for the tomato or “Pomodoro” kitchen timer that the technique’...student.   The Pomodoro timer is literally a tomato! Image from Gerlos.   Tools for checking grammar...with others which makes it easier to work with collaborators. Zotero is a desktop based program that’s compatible...

  17. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ...to create gene deletions of important unknown regulatory regions in Pseudomonas and Klebsiella spp. The...Ibadan, Nigeriaunder supervision of Dr. Akindele Oluwatosin Adeyi, University of Ibadan, Nigeria Gloria Otito...Candidate working under the supervision of Akindele Oluwatosin Adeyi at the University of Ibadan. She works ...

  18. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...culture and include a counter, lab timer, dilution calculator, and a couple of other nice features. This app...sequence for your enzyme of interest. Lastly, the Tm calculator helps you set up an optimal PCR depending on ...apps: Findings Lab Notebook Cell Culture Split Calculator by DRDP Restriction Enzyme Tool by Promega Material...

  19. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...histidine kinase that phosphorylates a response regulator (RR). TCS-induced responses are varied; one outcome...conditions, the chromophore activates the response regulator via phosphorylation, promoting transcription of...spread across three plasmids, with the response regulator encoded in the genome. To make the system less...

  20. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    ...identified other advantages, including Cas13d-specific regulatory proteins and high targeting efficiency, both ...WYL domain-containing proteins may be broader regulators of Cas13d activity. This property makes WYL proteins...REPAIR Get tips for using Cas9 Transcriptional Activators Learn about Cas13a (C2c2) Characterization Resources...

  21. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...sponsored the capital construction of our new BL2+ laboratory and the infrastructure capabilities to produce...our generous, sharing depositors. Over 3,000 laboratories are sharing plasmids (with 16 labs currently...helping thousands of sharing scientists from laboratories all over the world. With this reach and exposure...

  22. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...effectively contribute as part of a team.” As a seasoned educator, Kuldell also knew that the best days in the classroom... or small, receive the BioBuilder textbook, a laboratory kit, and support during the academic year from...state-of-the-art lab embedded in LabCentral’s community of innovators that opened in August 2017.  This flexible teaching...

  23. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    ...of stakeholders from industrial and commercial operators, government agencies, active researchers, as well...understanding of the societal ramifications of laboratory science, and how it may best be leveraged for...re always delighted to engage with potential collaborators and interested audiences. Please visit our team...

  24. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type
    Blog Post
    ...in Huntsville explains it, a major goal in his laboratory is to understand the function of the non-coding...effort to track down every last one of those gene regulatory proteins and the places in the genome where they... that, but the system is so flexible. Other investigators can easily adapt these vectors for use with ...

  25. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...containing the RNA pol III promoter, gRNA and pol III terminator. These relatively large cassettes (considering...Size is also a factor; the pol lll promoter and terminator need be encoded only once, and each <200 bp tRNA-gRNA...deletions of other regions, such as noncoding RNAs or regulatory elements, to examine their function. Bacteria...

  26. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    ...(Shaner et al, 2008). Obligate dimers (such as dTomato) that promote interactions between the FPs interfere...CytERM-mKOkappa, CytERM-mTurquoise2, and CytERM-dTomato. When using and interpreting the results of the...proteins. Fusions of CytERM with mTurquoise2 and dTomato, which are established monomeric and dimerizing...

  27. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    ...reporting and genetically encoded fluorescent voltage indicators (GEVI) are ideal tools to track bioelectric states...channelrhodopsin used as a sensitive fluorescent voltage indicator with near-complete desensitization in continuous...Use of genetically encoded, light-gated ion translocators to control tumorigenesis." Oncotarget 7.15 (...

  28. Developing Lab Management Software for Biology

    Type
    Blog Post
    ... Laboratory management software is not a requisite for a functioning lab, but it is for a scaleable lab...about what steps are most likely to fail. Our laboratory manager would definitely be interested to see...this case, the physical considerations of the laboratory make object composition a smarter choice than...

  29. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...Library, deposited by Carrie Bhang, a research investigator in the In Vivo Pharmacology group at Novartis...ClonTracer Since starting her new role as an investigator in the In Vivo Pharmacology group at Novartis...Addgene community! Carrie Bhang is a research investigator in In Vivo Pharmacology at Novartis Oncology...

  30. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...with a terminator. A simple TU could be comprised of just three parts: promoter, gene, and terminator.   ...this category also includes promoters, UTRs, terminators, etc. Once selected, individual units can be ...
Showing: 181 - 210 of 642 results