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We narrowed to 881 results for: NSI

Showing: 301 - 350 of 881 results

  1. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...gRNAs! Comic by Maya Kostman.   Important considerations before you start an experiment with CRISPR ...expression. Location and sequence are important considerations for designing your gRNAs. For indels, it's ...off targets. For CRISPRa and CRISPRi, these considerations are of roughly equal importance (target should..., 2020). is not limited to single nucleotide transitions but still requires a nearby PAM, although these...The off-target activity of gRNAs is important to consider. While the basic landscape of mismatches that ...

  2. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...the Moloney Murine leukemia virus (MoMLV) and consisted of two plasmids: the packaging plasmid containing...vectors Although retroviruses are still in use, considerable efforts have been devoted to develop efficient...generation. The 1st generation of lentiviral vectors consisted of three plasmids encoding 1) the lentiviral vector... from one batch to another. For experimental consistency it’s a good idea to purify and titer your virus...sucrose to the supernatant to create a gradient of density that favours virus precipitation. My tip for this...

  3. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...FLEx Vectors Important Considerations When Using AAVs Three Key Considerations For Precise Neuronal Targeting...-target expression.  Some classes of neurons transiently express TH during development or have low levels...titer to prevent off-target expression of the sensitive FLEx-split TVA receptor used for tracing neural...recombinase-dependent AAV vectors and mitigation with cross-over insensitive ATG-out vectors. Proc Natl Acad Sci USA 116:27001...

  4. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

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    Blog Post
    ...for a growing organism to make the complicated transition from a clump of cells to a fully differentiated...cooperate to cause a higher-energy electronic transition in a fluorescent molecule (see figure to the ...technical specificities have to be taken into consideration especially in the use of fluorescent proteins...wavelength can significantly affect emission intensities from fluorescent proteins (Salomonnson et al....Marcello, et al. "Dynamic imaging of dendritic cell extension into the small bowel lumen in response to epithelial...

  5. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    ...before you even receive the product? How can we consider the environmental impact of those processes? Who...laboratory products that emphasizes Accountability, Consistency, and Transparency. This label scores products...might sound risky in terms of contamination, but consider flow cytometers. These fluidic machines handle...through an EPA-approved process and no longer considered hazardous. Then, they use this material to produce...

  6. Viral Vectors 101: Systemic Capsids

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    Blog Post
    ...goes beyond such restrictions, you may want to consider a systemic capsid for your AAV.   Systemic capsids... definition of systemic capsids. After some consideration, we offer this definition: A systemic capsid...capsids are engineered, as well as highlight some considerations for their use. Then, we’ll provide information...and/or its intermediate engineered precursor. Considering systemic capsids for your research? While systemic...payload — there are a few additional, specific considerations you’ll need to be aware of before starting ...choice on which systemic capsid to use. Factors to consider include the delivery method, mouse strain, target...recommend you check out Caltech’s CLOVER Center’s extensive repository of published systemic AAV experiments...

  7. A new platform for fast functional characterization of potentially cancerous mutations

    Type
    Blog Post
    ...time consuming and expensive, especially when done in vivo. There are also sensitivity issues when attempting...them remain uncharacterized. This represents a considerable barrier to furthering research and developing...Atlas (TCGA), a catalog of genetic mutations responsible for cancer from genomics data, and large-scale...

  8. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...offer more information on these regions.   Cas9’s transition from weak, random binding to precision gene editing.... This cap is where the DNA:RNA heteroduplex transitions back to DNA duplex, and it’s the reason guide...RuvC loop caps the heteroduplex and marks the transition back to a DNA duplex. sgRNA, dark blue; target...DNA strand, and Cas9 bends the DNA helix by transitioning between the open and closed conformations. This... spots—to these questions. Each new structural insight also spurs the next generation of engineered CRISPR...

  9. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    ...  Joining the Addgene crew!  This pattern was consistent: everyone I spoke with at Addgene, no matter ...been done at Addgene before. This meant I was responsible for establishing a new report and routine across..., and make the occasional TikTok. I was also responsible for typical entry level tasks such as stuffing...connections with coworkers seem, in retrospect, nonsensical. I have found the people at Addgene have become...

  10. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ...need to be inside of a gene’s open reading frame, in fact, they can be activated a considerable distance... These reporters have historically been used extensively in fly, mouse, and yeast models. Why traps are... endogenous gene expression are all factors to consider. Once you’ve decided on the type of trap, there...

  11. Advice for Choosing a Research Project

    Type
    Blog Post
    ...interests. We've outlined some important factors to consider and summarize helpful advice from the successful...Achieve Success in Academia". Important factors to consider when choosing a research project After joining... your professional brand. It offers colleagues insight into your interests and will determine how you ...fungal biology.” Connie Cepko advises students to consider their goal of PhD training when selecting a research...

  12. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    ...Addgene blog Important Considerations When Using AAVs Three Key Considerations For Precise Neuronal Targeting...experiment, read our blog post on important considerations when using AAVs. Experimental results: These...Frontiers in Neuroanatomy and summarized some considerations for precise neuronal targeting using AAV on...

  13. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    ... genetic condition leaves patients incredibly sensitive to sunlight and prone to developing metastatic... into XP genes and other genes involved in photosensitivity in melanocytes with the goal of understanding...explore the functional differences among mutations responsible for the disease known as Alternating Hemiplegia...disorder.” In 2012, researchers identified the gene responsible for the disease as ATP1A3. The gene encodes a...

  14. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ...next and what options lie ahead of me? Like a considerable number of researchers, I had high hopes of securing...thought they would focus on “Has this person been consistent throughout their career?”, "Have they sought ...interviewed for 1 hour so having a broad enough insight was going to help me push both my scientific and...promotions or further education may allow you to transition into various realms of business.  Thank you ...

  15. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    ...do you make the most of this new relationship? Consider adding formality and active goal setting to your...major advantage of working with a mentor.The responsibility of arranging meetings and setting the direction...meetings and discuss these in person. See the extensive resource list available from this post. Use an...items and make progress. To illustrate this point, consider that I try to exercise every morning, but the ...

  17. Resume Writing for Non-academic Science Careers

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    Blog Post
    ...of the University of British Columbia. When transitioning from an academic science career path to a non-academic...applying for the position?   Here are a few things to consider when you're preparing your resume for a non-academic...postings and look at the jobs you are interested in. Consider listing the skills you already have, and the skills...Originally on track for a research career, she transitioned into a communications role instead after she...

  18. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    ...the plasmids we distribute. This method is time-intensive, so we recommend a variety of ways to screen and...10kb, with the 3.0kb fragment having increased intensity to serve as a reference band. The uncut DNA (lane...accurately visualize the full size of your construct. Consider buffer and temperature compatibility when digesting...methylation issues. Enzymes like XbaI and ClaI are sensitive to methylation and their activity may be blocked...

  19. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    ...positive population is no higher than 105 signal intensity. Signals any higher than this may not be clearly...comparison, say somewhere between a 104–105 signal intensity. If your samples and controls were processed ...better. The histogram shows peaks of high signal densities, so signal shifts due to upregulation or downregulation... shifts in signal using the mean fluorescence intensity (MFI) of the different populations. MFI is the...

  20. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type
    Blog Post
    ... Directors brings complementary experience and insight to the now 10 member board at Addgene. Let’s meet...perspective, what do you think are important considerations for the future of open science? What about ... he leads the Internal Audit function and is responsible for bringing a systematic and disciplined approach...Addgene blog Learn more about about Addgene in our "Inside Addgene" blog series Subscribe to the Addgene blog...

  21. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...Institute were instrumental in helping us establish consistent, high-quality protocols for lentiviral production...Janelia Research Campus have provided valuable insight into AAV production. Finally, we are blessed to...vector project team develops methods, performs extensive quality control, generates  educational materials... ship internationally and be ready to provide extensive logistics support for export and shipping on dry...

  22. AAV Q&A with Tim Miles

    Type
    Blog Post
    ... kB, then you would be oversized even before considering the need for some manner of polyA. In this scenario...dosing, we advise that users keep titering method consistent across samples within a project. Targeting Your...experiments using PHP.eB in the brain give some insight into codelivery efficiency at different doses. ...best practices’ for model organisms.    Q11: How consistent is expression is when injecting CAP-B10 or CAP-B22...

  23. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    ...using sequence homology is also common. Overlap extension PCR is used to splice together two PCR amplicons... multiple fragments, but these methods can be expensive because they require particular enzymes, some ...recombineering proteins Red/ET from a temperature-sensitive plasmid. Homologous recombination then joins the...this insertion size, primer synthesis is more expensive than gBlock synthesis, and multifragment assembly...

  24. To Each HIS Own

    Type
    Blog Post
    ... 2000).  One downside of polyhistidine tag-based purification, however, is the propensity for nonspecific...contamination, such as mammalian production systems, consider using these more selective substrates along with...the opposite end.  If neither placement works, consider purifying under denaturing conditions. Unlike ...

  25. All in a Twist: dsRNA

    Type
    Blog Post
    ...process. These transcription-coupled R-loops are transient in nature and will dissociate when gene transcription... respond accordingly. While not perfect, these sensing systems work to distinguish between RNA of cellular...viral origin and respond appropriately. When these sensing systems fail, diseases and steep cellular consequences...or anticipating this response are important considerations when using these reagents. RNA:DNA hybrids...

  26. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...clinical applications could be considered. It is important to consider the question of broader applicability...disease prevention. However, it is important to consider that already-tested CRISPR alternatives are applicable...many more experiments are needed before we can consider clinical applications for germline editing. Although...

  27. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ...to a large family of solute channels that are responsible for maintaining the nutrient and ionic balance...says Iyer. Since the RESOLUTE plasmid collection consists of human and murine solute channels, the team ... University.  These protein-receptor complexes consist of many parts that work together on the cell membrane...transduction events, we will utilize BRET-, FRET-, and intensity-based signaling biosensors to monitor intracellular...

  28. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...vector + insert may spontaneously religate, this transient construct retains functional Type IIS sites and...be scarless. Golden Gate assembly is also less expensive than many commercial cloning methods. Golden ...amenable to "domestication", you might want to consider using an alternative method like Gateway cloning...cloning or Gibson assembly. Another important consideration is the design of flanking overhangs. Although...

  29. Working with Nuclear Receptors

    Type
    Blog Post
    ... multitude of therapeutic indices, including hypertension, cancer, diabetes, cardiovascular disease, cholesterol...receptor research, and has generated a set of comprehensive datasets that collectively address many important...of coregulators in HeLa cells was the first comprehensive analysis of the interactome of these key regulatory... between laboratories. The development of the transient transfection platform for assaying transcriptional...

  30. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ... in E. coli. The system available from Addgene consists of two plasmids: pCas9: carries Cas9 and chloramphenicol... enable gRNA-directed transposition. INTEGRATE consists of four major components including (1) a CRISPR... ability to multiplex repression. Using a Tet responsive promoter to drive expression of a catalytically...and developed new, more tightly controlled, Tet responsive promoters to avoid leaky activation of the system...technology can have on the human microbiome. You can consider the microbiome as a collection of organisms, their...

  31. Design Tips for Prime Editing

    Type
    Blog Post
    ...a prime editing guide RNA (pegRNA). The pegRNA consists of a CRISPR gRNA extended to include a primer ... BioRender.com. The first base of the 3′ extension of the pegRNA should not be C. A C base is speculated...al., 2014). Figure 3: pegRNAs whose 3′ extension begins with a C base can cause unwanted base pairing...influence the success of your prime edit. If possible, consider following these suggestions: Edit the PAM along...

  32. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...categories: probes and editors. A probe array may consist of at least two sensor modules targeting a specific...auxin inducible degron (AID) system has been extensively used in C. elegans since 2015 to conditionally... which can be easily visualized.  The tool-kit consists of highly useful vectors compatible with Gibson.... Check out the new additions.  Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f, now...

  33. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    ...time moaning about their troubles, Silent or unresponsive clams, who seem to ignore you and your questions...communicate their ideas confidently while also considering the needs of other people. Some students and ...for it. Do not take criticism personally or get defensive. Look at the problem from their viewpoint, and... you even when you meet in person (silent or unresponsive clam), you will probably need support from your...

  34. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...which spectral flow cytometry requires special considerations. Conventional flow cytometry During conventional...cytometry for a given experiment, it is important to consider how many parameters you want to use in a given...and spectral flow cytometry, it is important to consider the expression levels of the markers you are including...molecules conjugated together) can be especially sensitive to batch effect. For example, when a PE molecule...

  35. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...Overall the general strategy for protein expression consists of transfecting cells with your DNA template of... Mammalian systems can express proteins both transiently or through stable cell lines. Both methods produce...recombinant proteins from plants however can be expensive and time consuming as plant tissues themselves...applications. Cell-free expression systems however, are expensive and very technically challenging to use. Alyssa...

  36. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...gotten larger, easier, and more comprehensive thanks to the consistent development and improvement of ... for each experiment. Additional considerations One of the downsides to using AAV is the limited packaging...

  37. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...The pegRNA consists of a target-complementary spacer, a scaffold sequence, and an extension sequence containing...screens such as PRIDICT/PRIDICT2.0 provide valuable insights into pegRNA editing efficiencies for specific ...experiments, thus saving you time, sweat, and money on extensive optimization efforts. This holds true for any ...

  38. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...most starting materials, and is not particularly sensitive. The fluorescent signal can be affected by AT-...Image courtesy of: Ygonaar    Taqman is more sensitive than Sybr, with less background noise, and is ...amplified in the same reaction. Taqman’s increased sensitivity allows for a more confident determination of ...approach is right for your experiment. You’ll have to consider:  Resources needed Most molecular biology labs...

  39. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type
    Blog Post
    ...all seriousness, EGFP/GFP has a real nontrivial propensity to noncovalently dimerize. That means that your...to not produce artifacts in your experiments. Considerable effort has gone into making FPs monomeric, yet...actually monomeric, at least in practical terms. The propensity of a particular type of molecule to form a dimer...study of GFP-based FRET, with application to intrinsically unstructured proteins. Protein Sci, 2007. 16...

  40. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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    Blog Post
    ...RNA-guided gene drives in the summer of 2014 (after extensive discussions with biosecurity experts, ecologists...gene drives intended for eventual release should consider building an appropriate immunizing reversal drive...not use them whenever applicable?Against this, consider the cost of an accidental release. Science relies...trusted to handle such a powerful technology responsibly. An accidental release would be particularly ...

  41. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...DDBJ, and PDB sequences and may be the most comprehensive for searching. Timesaving Tip #1: If you know...searches! Now, before you click the BLAST button, consider the Program Selection parameter, as this will ...the alignment at a more distal amino acid. We consistently run our blastx searches with the “Low complexity...101: How to Verify Your Plasmid Plasmids 101: An Inside Look at NGS Plasmid Quality Control Learn about...

  42. An Introduction to Adenovirus

    Type
    Blog Post
    ...translation. Its genome consists of “early” (E) and “late” genes, and the transition between early and late...length. Human adenovirus type 5 (hAdV-C5) can be considered the prototype, although many viruses infecting...the majority of adenoviral vector expression is transient due to the fact that AdV surface proteins (especially...

  43. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...specific genomic region using the CRISPR system, consisting of dCas9 and targeting gRNA, to identify molecular...isolation of a specific genomic region was an intensive process. One method required insertion of a recognition...vitro" form. In this form, the CRISPR complex, consisting of a recombinant dCas9 protein and synthetic ...high-affinity purification. This system is both highly sensitive and specific and allowed the Xu lab to identify...

  44. AAVs in Retinal Gene Therapy

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    Blog Post
    ...abandoned early on due to its considerable immunogenicity and its transient expression. LV results in long...been shown to to transduce the retina but only transiently. However new viral vectors capable of providing...disease-causing genes, including the RPE65 gene responsible for an autosomal recessive, severe, childhood-onset...

  45. Negative Can Be Positive: Open AAV Data with Addgene

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    Blog Post
    ...Working with AAV vectors in neuroscience Let’s consider my first challenge when I started working with...and that it often provides incredibly valuable insight into one’s work. As a community, we must therefore...., Grames, M. S., & Klein, R. L. (2018). More expansive gene transfer to the rat CNS: AAV PHP.EB vector...with Addgene’s AAV Data Hub!Data Freedom: The Expansion of Data Sharing in Research Publications...

  46. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...method into six general steps and outlined the considerations to be made during each step. Now that you know...method of fixation is good for epitopes that are sensitive to cross-linking, but it doesn’t preserve sample...binding to any target protein that is still on the inside of the cell. However, assuming you do need to permeabilize... then optimizing from there is a good option.  Consider your target - if you are studying a membrane protein...

  47. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    ...data with a 7-day rolling mean gave us a crude insight into the magnitude of the effect COVID-19 had on...holiday effects. Based on these observations, we considered the following (noiseless) model for our data:...factor 𝜙z if t is during event z. The "events" we considered for our model were weekends, Christmas/New Years...Europe. To improve the stability of our model, we considered only the 10 papers with more than 10 unique order...

  48. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    ... in transit and some have been transported across oceans and continents. Each deposit may consist of anywhere...your lab ensure that sample ID’s are unique, consistently labeled, and that numbers are not switched around...Resources Avaliable on the Addgene Blog Read more Inside Addgene Posts! Learn all about Plasmids in our ...

  49. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...research grants, especially in the sciences and by extension to scientifically oriented nonprofits. Like Deb...career path changed when I was asked if I would consider consulting for a particular grant by another state...entering the Grants Profession. Whether you’re transitioning from a previous career or you’re starting your...above and formalizes your development. You may consider earning a degree in Grant Writing, Management ...

  50. Plasmids 101: Protein tags

    Type
    Blog Post
    ...these challenges, researchers have amassed a considerable molecular tool box of tags and fusion proteins...deploy proteosomes to degrade what the cell might consider junk protein. Though there are a number of chemical...EINAPTDGKV EKVLVKERDA VQGGQGLIKI GVHHHHHH  9 Combo Consists of a bacterially derived in-vivo biotinylation...and Gly residues   This article is not a comprehensive guide to all tags, but rather a quick overview...
Showing: 301 - 350 of 881 results