Skip to main content
Addgene
Showing: 301 - 350 of 649 results

  1. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ...strong collaborative research team motivated by similar visions and goals? We continue our conversation...and a friend. George Church runs his lab fairly similarly to his advisor Wally Gilbert: “Very interdisciplinary...

  2. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...can either express the plasmid DNA transiently (similar to bacteria) or incorporate the genetic material.... Positive selection in mammalian cells works similarly to that in bacteria and a table of the most commonly...

  5. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...Packaging using 293T cells This method is very similar to lentiviral packaging methods. The three components...constructs. Why do many γ-retroviral plasmids have similar names? The nomenclature of the plasmid backbone...

  6. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ... an advanced search option. For the former, you simply type in identifier keywords (Cas name, RefSeq ID...phylogenetic nomenclature and discover orthologs with similar properties (Makarova et al). CasPEDIA is an evolving...

  7. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...organisms. This is often cDNA, shRNA, or something similar, and is contained on the transfer plasmid. If expression...different from gamma-retrovirus, but they perform very similar functions to infiltrate the host cell and deposit...

  8. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ...management, and how does it relate to science? In the simplest of terms, a software product manager helps ensure...could code the best operations software possible. Similarly, when the developers needed to know information...

  10. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...this post in the graphic below - including some simple comparisons. The easy multiplexing afforded by...integrated RNA and CRISPR/Cas toolkit in human cells. Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK. ...

  12. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...Assembly, Golden Gate etc.), and try to find the simplest way to do it (i.e. avoid assembling too many fragments...help you at this stage and that can be used to simulate in silico cloning. This will definitely help in...

  13. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...Fandino, L. B., Labasky, M. E., Forostyan, T., Colosimo, L. K., Graham, T. E. (2015). Rapid parallel measurements...Fluorescent Protein Oligomerization Seeing Red: Simple GFP Photoconversion Light Sheet Fluorescence Microscopy...

  14. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...With these improvements, labeling efficiency was similar to that of a comparable FISH method. Importantly...By labeling two genes of a given chromosome simultaneously, they could also monitor the spatial relationship...

  16. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...experiences. If you’re hearing multiple people with similar misconceptions, it’s time to rework your strategy...group was just really tired and needed a slower, simpler presentation than I had planned; other times students...

  17. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...variables (scFvs) are ~27kD engineered fusion proteins similar to a Fab fragment but created through engineering...different antigen; binding of each can be either simultaneous or independent of the other (Shahied, 2004)....

  18. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...successfully used in conditions that are the most similar to yours. Take a look at the publications cited...application, a publication successfully used it in a similar way as you plan to. To secondary or not to secondary...

  19. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...conjugated in the lab. In the indirect approach, you can simply keep a stock of conjugated secondary antibodies...regardless of the number of protein targets, simplifying multiplex assays.  A brief note Since the indirect...

  20. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...getting too nervous or having a memory lapse, I simply pause and take a deep breath before the panic sets...make a strategy that can help. Even something as simple as telling yourself, “I can stop and check my notes...

  21. All in a Twist: dsRNA

    Type
    Blog Post
    ...been clinically exploited for antiviral purposes similar to that of vaccines. If you know the sequence of...responses can mount in the absence of infection. Similarly, if endogenously synthesized dsRNA finds its way...

  23. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...protein fusions, and multiple cloning sites. In the simplest case we have a standard backbone with one insert...reactions in parallel. We have a few tricks to simplify the process. First, nearly all of our Sanger sequencing...

  24. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...actually really research oriented [and] somewhat similar to an academic research environment” with a strong...multiple replicate populations, they found that similar barcodes consistently made up the majority of the...

  26. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ...their parents. Here’s how to cross mice, using a simple example with the goal is to generate mice that ...raised in the same environment and will harbor similar epigenetic modifications. Special breeding cases...

  29. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...plasmid deposit process can be broken down into four simple steps: Send plasmid information to Addgene Material...the Deposit Spreadsheet is the quickest option. Simply download the spreadsheet and copy and paste your...

  30. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ... For some transgenes, transfection optimization simply is not enough to get a sufficient titer; this is...volumes of lentivirus routinely, you may opt for simply increasing the number of 10 cm2 dishes. While this...

  33. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...task, action, and results. While this method seems simple, I found it hard to remember to describe all four...answers rings true for you, go ahead and give a similar answer in a following interview. Expect to be tired...

  36. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...cutting most anywhere within the RNA will do. Similarly, if you want to knock out a gene, a frameshift...enzymes   The solution to the PAM problem seems simple, in theory: find a Cas enzyme or evolve one that...

  37. CRISPR in the Clinic

    Type
    Blog Post
    ...therapy currently in clinical trials is somewhat similar (Hussein et al). It employs Cas9 to cut at two ...therapy, since TTR is primarily made in the liver. Similarly, for cardiovascular disease approaches targeting...

  38. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...researcher to come into contact with than GFP. Similarly, if the viral vector carries an shRNA to a gene...classified as a BSL-1 safety level. Adenovirus is similar to AAV in that it does not integrate into the host...

  39. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ... better for probing poorly expressed targets. Similarly, rabbit derived antibodies may be the better choice... from each other. In a direct approach, that is simple - but what about when you need to use an indirect...

  42. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...CRISPR systems based on Cas9 were thought to be the simplest CRISPR systems and the easiest to adapt to genome...et al. suggest that AsCpf1’s off-target rate is similar to that of high fidelity Cas9s eSpCas9 and SpCas9...

  43. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...OpenWorm project. The main goal of OpenWorm is to simulate C. elegans as a computer model. When I first heard...genome. While C. elegans are often thought of as simple organisms , they have many levels of complexity...

  44. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...transfer plasmid. The good news is there’s a few simple ways to deal with this issue. One way is to grow...cultures with intact ITRs. I use a spreadsheet similar to this one to plan my AAV packaging PEI transfections...

  46. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...insect cells require demanding culture conditions similar to mammalian expression systems. Bacterial expression...expression. Protein expression in bacteria is quite simple; DNA coding for your protein of interest is inserted...

  48. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...End point PCR, which is commonly referred to as simply PCR, is used for an array of different molecular... or pathogen identification. Scientists can simultaneously detect several different pathogens in one specimen...

  49. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...jbc.RA120.01289     Novel protein cage provides simple enzyme protection in yeast bioproduction pathways... a reaction pathway, the MPyV system provides a simple, efficient method for protecting a single enzyme...

  50. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ... helper viruses like adenovirus (Ad) or herpes simplex virus to facilitate efficient replication. The ...purify your rAAV from your supernatant, you can simply use polyethylene glycol (PEG). We add 25 mL of ...
Showing: 301 - 350 of 649 results