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  1. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ... PCR followed by Restriction Fragment Length Polymorphism (RFLP) analysis (right). Introduction of restriction...of that particular site with fragment length polymorphism (RFLP) analysis. In this process, if your mutation...

  3. Antibodies 101: Validation

    Type
    Blog Post
    ...This is a good option for applications like immunohistochemistry on human tissues, when (1) other strategies...correspond to protein levels, so you perform immunohistochemistry with your antibody on these tissues (B)....

  4. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...Antibodies can be used to visualize tissues (immunohistochemistry) or cells (immunocytochemistry). This could...Arbabi-Ghahroudi M (2017) Camelid Single-Domain Antibodies: Historical Perspective and Future Outlook. Front Immunol...

  5. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...reversible photoactivation - and have enabled sophisticated imaging techniques.     Irreversible photoactivation...victoria) GFP (wtGFP) by mutating Threonine 203 to Histidine (T203H) (1). wtGFP normally contains a mixed population...

  7. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ... today, we’ll start with a CRISPR overview and history. Tuesday will focus on generating knockouts using...

  8. The Addgene Antibody Data Hub

    Type
    Blog Post
    ...application — flow cytometry, western blot, immunohistochemistry, immunocytochemistry, ELISA — but in all...

  13. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    ...Feng Zhang lab identified a Cas12b from Bacillus hisashii, BhCas12b that is functional at 37 °C but preferentially...

  23. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...Arbabi-Ghahroudi M (2017) Camelid Single-Domain Antibodies: Historical Perspective and Future Outlook. Front Immunol...

  39. Science Career Options

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    Blog Post
    ...hobbies and volunteer experiences, as well as work history can really show that you have some of the abilities...

  41. Components of CRISPR/Cas9

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    Blog Post
    ... to modify the desired genomic locus.   Some historical notes CRISPR arrays in bacterial genomes consist...

  43. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...of the biosensors used in research are very sophisticated, but at their core they can all be boiled down...

  44. Antibodies 101: Isotypes

    Type
    Blog Post
    ...antibody-based applications including flow cytometry, immunohistochemistry, immunocytochemistry, ELISAs, and Western...

  47. Antibodies 101: ChIP

    Type
    Blog Post
    ...antibody on crosslinked proteins. In the case of histone modifying proteins, which bind quite strongly to...

  48. Hot Plasmids - February 2022

    Type
    Blog Post
    ...separately. The proteins, which are purified via histidine tags, are optimized for SDS-PAGE gels and can ...
Showing: 301 - 350 of 414 results