...ones? Many of the available biosensors use a single GFP or CFP/YFP-derived FRET pair. If you need to free...targeting. By employing various AAV serotypes, researchers can precisely direct the calcium sensor to specific...complex brain circuit dynamics in vivo. Neuroscience Research. 2021 Aug 1;169:2-8. Suzuki J, Kanemaru K, Iino...

...Expression with Cre-lox Control Protein Activity with GFP Resources at Addgene.org Find KTR reporter plasmids...photoswitchable kinases in vivo with C. elegans. Researchers commonly use rectal bacterial infection to induce...

... enriched relative to controls using Split-BioID-GFP and BioID run on six unrelated proteins. One caveat...half-lives. Interested in using Split-BioID with your research? Plasmids are available from Addgene! References...

...Addgene has several great options: pLV hUbC-dCas9-T2A-GFP for removable dCas9 expression and pLM-CMV-R-Cre....p300 or the DNA methyltransferase DNMT3A allows researchers to easily add biochemical marks to genetic regions...

...contrast, the widely used green fluorescent protein (GFP) gives a bright green fluorescence when exposed to...luminescence have been widely used in biological research for a long time, chromoproteins are just now being...history of fluorescent proteins in molecular biology research, chromoproteins have a shorter history. In 1987...eforRed, asPink, spisPink, scOrange, fwYellow, amilGFP, amajLime, cjBlue, meffBlue, aeBlue, amilCP, tsPurple... can be used, why not add some color into your research? If you have a favorite chromoprotein, either ...Addgene.org Find fluorescent proteins for your research Read our Molecular Biology Reference Find plasmids...

..., Myc and SV40 Nuclear Localization Signals, and GFP. B) Workflow to assess Cas9-PAGE editing with viral...fluorescent proteins derived from StayGold. ResearchSquare (Preprint). https://doi.org/10.21203/rs.3.rs... brilliance By Hridaya Patel (Addgene co-op) Researchers in the Gradinaru lab have developed an engineered...

...select both over- and under-expressing clones. If GFP-encoding cDNA is co-transfected at a favorable ratio... biological screens. For example, for cancer researchers, if growth properties in agarose are of interest...

...199419 Anti-GFP [N86/38R-2b] GFP Aequorea victoria Mouse IgG2b 199420 Anti-GFP [N86/8R-2b] GFP Aequorea ...206719 Anti-GFP [N86/38R-1] GFP Aequorea victoria Mouse IgG2a 206720 Anti-GFP [N86/8R-1] GFP Aequorea victoria...Rat Mouse 206743 GFP scFv [N86/44] N86/44 scFv GFP Aequorea victoria Mouse 206744 GFP scFv [N86/20] N86...short and long Rat Mouse IgG2a 114492 Anti-GFP [N86/38.1R] GFP Aequorea victoria Mouse IgG2a 114493 Anti-NGL...glutamate receptor Rat Mouse IgG2a 177572 Anti-GFP [N86/8R] GFP Aequorea victoria Mouse IgG2a 177573 Anti-...N479/107R] VAPA/B Mouse IgG2a 188164 Anti-GFP [N86/44R] GFP Aequorea victoria Mouse IgG2a 188165 Anti-.../22R] Prrt2 Mouse Mouse IgG2a 188166 Anti-GFP [N86/20R] GFP Aequorea victoria Mouse IgG2a 188167 Anti-...

...Mammalian Cells Learn about the Dangers of Using GFP for Protein Localization Resources on Addgene.org...Biotechnology and Biological Sciences Research Council (BBSRC) and Medical Research Council (MRC) require authentic...the amount of money wasted in basic oncological research due to contamination are in the tens or hundreds...lines deposited with them and used by different researchers are free of contamination and match their original...perhaps a working cell bank has been made by a research institution (an excellent practice), or maybe ...Warwick University, gaining extensive cell culture research experience through working for a variety of pharmaceutical..., HeLa cells, and cell culture contamination." Archives of pathology & laboratory medicine 133.9 (2009...

...used this cassette to knock out genes, knock in a GFP cassette, and introduced site-directed mutations,...selection. In a subcutaneous cancer model, the researchers showed that after 24 hours, the unstabilized ...in nature, these systems have been adapted by researchers for many uses when plasmid retention without ...for long-term experiments. They’ve also helped researchers design and optimize cloning methodologies using...genetic modification of Pichia pastoris." FEMS yeast research 9.4 (2009): 600-609. PubMed PMID: 19416369. Kulakauskas....org Check out our molecular biology reference Search for a plasmid here Find plasmids from our special...

...Lindsey M. Costantini, and Erik L. Snapp. "Superfolder GFP is fluorescent in oxidizing environments when targeted... in Biomedical Research from Albert Einstein College of Medicine. Her doctoral research was conducted ...Figure 1). When behaving properly, FPs enable researchers to investigate the localization and dynamics ...co-mentorship Blossom Damania and Jack Griffith, her research focuses on the replication machinery of Kaposi...secretory pathway."Biochemical and biophysical research communications 430.3 (2013): 1114-1119. Pubmed...

...Fluorescent Protein Aggregation Catch Up On Your GFP Basics Get Advice on Choosing Your Fluorescent Protein...to monitoring localization; using FP timers, researchers can look at a single image of a cell and understand... production of the initial FP timer in 2000, researchers have realized how problematic aggregation-prone...expression. In a developing embryo, for example, a researcher could determine whether or not newly synthesized...synthetic biology, FP timers might be able to tell researchers whether old or new cells are better at producing...

...which must be stabilized by monomeric superfolded GFP and averages only ~50% RNA knockdown. In initial ... doors for scientific research - how will you use these systems in your research? Cary Valley contributed...developed and potential ways you can use it in your research. RNA editing advantages RNA editing has multiple... forward to the expanded use of RNA editing in research and its potential therapeutic uses We are also...

...like Luciferase and GFP Resources on Addgene.org Find Plasmids for Your Research     ...not a big issue for cells, but it may confound research results or even kill your cells if your gene of...right tissue-specific promoters as described by researchers at the NIH here.  Note: A. Max Juchheim contributed...

...mutation can be introduced in a gene, while adding a GFP-tag (Figure 3). Achieving multiple modifications...Molecular Biology. Jake Watson is a postdoctoral researcher in neurophysiology at the MRC Laboratory of Molecular...homologous recombination in vivo." Nucleic acids research 21.15 (1993): 3601. PubMed PMID: 8346047. PubMed...

...in this case a reporter gene (e.g. luciferase or GFP), regardless of whether it interacts with a transcriptional...that retain their individual activities (1). Researchers also discovered the modular format of some transcriptional...

... a superfolder GFP fusion to stabilize the protein in mammalian cells. LwaCas13a-msfGFP can mediate cleavage...genes that fell into 3 categories based on the architecture of the CRISPR protein in question: C2c1, C2c2...

...comprehensive comparison to GFP, please refer to NEB's comparison of SNAP-tag, CLIP-tag, and GFP . Technology SNAP-tag...are available at Addgene for distribution to the research community. Key features: Allows for visualization...

...dopamine research. CBA-driven, Cre-dependent expression of diphtheria toxin receptor (DTR)–GFP fusion protein... be useful for neurodegeneration research. Neurodegeneration... Neurodegeneration Research Collection Addgene has assembled...collections that may help with your neurodegeneration research. Disease Info Plasmid Collection CRISPR Tools ...studies (GWAS) or other large scale studies. Researchers are now investigating the role that these additional...additional genes may play in Alzheimer's disease. Research Tools Neurodegeneration Plasmid Collection A collection...monoclonal antibodies and scFvs for neuroscience research created with high-volume hybridoma sequencing ...

...Calcium erGAP3 (GFP-Aequorin Protein) for imaging of Ca+ dynamics in endoplasmic reticulum GFP-Aequorin Protein...FlincG3 (GFP-based cGMP sensor) for imaging in C. elegans neurons Using a Robust and Sensitive GFP-Based ...Janelia Research Campus. Online resource. 2020 GENIE Project Calcium jGCaMP7 High-performance GFP-based ...cyclic AMP) Signaling reporter island (SiRI) with GFP-based fluorescent reporter cAMPr Spatial Multiplexing... FLAMP Plasmids Jun Chu cGMP (cyclic GMP) FlincG GFP-based cGMP sensor Differential patterning of cGMP... vascular smooth muscle cells revealed by single GFP-linked biosensors. Proc Natl Acad Sci U S A. 2008...autophagic flux by pH-sensitive fluorescence (pMRX-IP-GFP-LC3-RFP) An Autophagic Flux Probe that Releases an...