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We narrowed to 204 results for: Arch-GFP

Showing: 121 - 140 of 204 results
  1. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...David Nemazee 161030 Zhou-pLOV-GFP-Luc Lentiviral vector expressing GFP and luciferase for use as a reporter...resistance. Christopher Vakoc 138152 pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro Lentiviral vector ...viral entry and infection. This approach allows researchers to safely study mechanisms of infection and to...coronaviruses using appropriate packaging plasmids. Use the search bar to find a particular viral spike protein (MERS...vector expressing destabilized EGFP. Neville Sanjana 164432 pHAGE-CMV-Luc2-IRES-ZsGreen-W Lentiviral dual reporter...
  2. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...199419 Anti-GFP [N86/38R-2b] GFP Aequorea victoria Mouse IgG2b 199420 Anti-GFP [N86/8R-2b] GFP Aequorea ...206719 Anti-GFP [N86/38R-1] GFP Aequorea victoria Mouse IgG2a 206720 Anti-GFP [N86/8R-1] GFP Aequorea victoria...Rat Mouse 206743 GFP scFv [N86/44] N86/44 scFv GFP Aequorea victoria Mouse 206744 GFP scFv [N86/20] N86...short and long Rat Mouse IgG2a 114492 Anti-GFP [N86/38.1R] GFP Aequorea victoria Mouse IgG2a 114493 Anti-NGL...glutamate receptor Rat Mouse IgG2a 177572 Anti-GFP [N86/8R] GFP Aequorea victoria Mouse IgG2a 177573 Anti-...N479/107R] VAPA/B Mouse IgG2a 188164 Anti-GFP [N86/44R] GFP Aequorea victoria Mouse IgG2a 188165 Anti-.../22R] Prrt2 Mouse Mouse IgG2a 188166 Anti-GFP [N86/20R] GFP Aequorea victoria Mouse IgG2a 188167 Anti-...
  3. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...Expression with Cre-lox Control Protein Activity with GFP Resources at Addgene.org Find KTR reporter plasmids...photoswitchable kinases in vivo with C. elegans. Researchers commonly use rectal bacterial infection to induce...
  4. New England Biolabs Cell-Imaging Plasmid Collection

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    Collection
    ...comprehensive comparison to GFP, please refer to NEB's comparison of SNAP-tag, CLIP-tag, and GFP . Technology SNAP-tag...are available at Addgene for distribution to the research community. Key features: Allows for visualization...
  5. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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    Blog Post
    ...contrast, the widely used green fluorescent protein (GFP) gives a bright green fluorescence when exposed to...luminescence have been widely used in biological research for a long time, chromoproteins are just now being...history of fluorescent proteins in molecular biology research, chromoproteins have a shorter history. In 1987...eforRed, asPink, spisPink, scOrange, fwYellow, amilGFP, amajLime, cjBlue, meffBlue, aeBlue, amilCP, tsPurple... can be used, why not add some color into your research? If you have a favorite chromoprotein, either ...Addgene.org Find fluorescent proteins for your research Read our Molecular Biology Reference Find plasmids...
  6. Hot Plasmids February 2024

    Type
    Blog Post
    ..., Myc and SV40 Nuclear Localization Signals, and GFP. B) Workflow to assess Cas9-PAGE editing with viral...fluorescent proteins derived from StayGold. ResearchSquare (Preprint). https://doi.org/10.21203/rs.3.rs... brilliance By Hridaya Patel (Addgene co-op) Researchers in the Gradinaru lab have developed an engineered...
  7. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...select both over- and under-expressing clones. If GFP-encoding cDNA is co-transfected at a favorable ratio... biological screens. For example, for cancer researchers, if growth properties in agarose are of interest...
  8. Tetracycline Inducible Expression

    Type
    Collection
    ...Iwasato 63704 pRetroX GFP T2A Cre Retroviral vector for dox-inducible expression of GFP T2A Cre recombinase...Eric Kowarz 96930 XLone-GFP Tet-On PiggyBac vector for inducble expression of EGFP Tet-On 3G rtTA TRE3GS...171123 pLVX-TetOne-Puro-GFP Lentiviral Tet-On vector for inducible expression of EGFP Tet-On 3G rtTA TRE3GS...pTet-IRES-EGFP Lentiviral plasmid for Tet-controlled expression of transgene of interest with EGFP (On or...transactivators, and vectors for other applications, or search our collection for all dox-regulated plasmids .... Off) None TRE, miniCMV Maria Lung 16542 pBI-MCS-EGFP Bidirectional promoter (Pbi) for Tet-responsive ...expression (On or Off) of both your gene of interest and EGFP. Pbi contains a TRE between two minimal CMV promoters...
  9. Bacterial Expression Systems

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    Collection
    ...Vladislav Verkhusha 52732 52733 pET11a-link-NGFP pMRBAD-link-CGFP GFP (reconstructed) BiFC Lynne Regan 168257...Fluorescence (GFP+) Gram-negative bacteria Philip Poole 14460 pOT2 Promoter activity Fluorescence (GFPuv) Gram-negative... Guide to Selecting Fluorescent Dyes and Ligands GFP Fusion Proteins — Making the Right Connection Photoactivatable...pRsetB-his7-Perceval ATP:ADP ratio Fluorescence (GFP) Escherichia coli Gary Yellen 187836 pVoPo-01 Promoter...bacteria Philip Poole 14083 pAKgfplux1 Promoter activity Fluorescence (GFPmut3a) and luminescence (lux operon...bacteria Attila Karsi 14076 pAKgfp1 Promoter activity Fluorescence (GFPmut3a) Gram-negative bacteria Attila...-M ATP Fluorescence (GFPmut2) Escherichia coli Rahul Sarpeshkar 111614 pCdrA-gfpC Cyclic di-GMP Fluorescence...
  10. Fluorescent Protein Guide: Biosensors

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    Collection
    ...Calcium erGAP3 (GFP-Aequorin Protein) for imaging of Ca 2+ dynamics in endoplasmic reticulum GFP-Aequorin Protein...FlincG3 (GFP-based cGMP sensor) for imaging in C. elegans neurons Using a Robust and Sensitive GFP-Based ...Janelia Research Campus. Online resource. 2020 GENIE Project Calcium jGCaMP7 High-performance GFP-based ...cyclic AMP) Signaling reporter island (SiRI) with GFP-based fluorescent reporter cAMPr Spatial Multiplexing... FLAMP Plasmids Jun Chu cGMP (cyclic GMP) FlincG GFP-based cGMP sensor Differential patterning of cGMP... vascular smooth muscle cells revealed by single GFP-linked biosensors. Proc Natl Acad Sci U S A. 2008...autophagic flux by pH-sensitive fluorescence (pMRX-IP-GFP-LC3-RFP) An Autophagic Flux Probe that Releases an...
  11. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Mammalian Expression sfGFP (superfolder GFP) 485 507 54 14 min Prone to dimerization sfGFP-N1 - Mammalian Expression...Expression EGFP 488 507 34 6 25 min Prone to dimerization pcDNA3-EGFP - Mammalian Expression pCAG-GFP - Mammalian...Expression PA-GFP 504 517 400 14 Monomer (A206K) pPAGFP-N1 - Mammalian Expression pPAGFP-C1 - Mammalian... pRSETA-PAGFP - Bacterial Expression pFA6a-PA-GFP-kanMX6 - Yeast Expression pFA6a-link-yEPAGFP-CaUra3 ...Brightness pKa Maturation Structure Plasmids Free Use GFP (fuGFP) 395 503 Monomer pUS252 - Mammalian Expression...high concentration. Proteins that are derived from GFP and contain the A206K mutation are monomeric at all...Structure Plasmids AcGFP1 475 505 28 Prone to dimerization AcGFP1-N1 - Mammalian Expression AcGFP1-C1 - Mammalian...
  12. The Challenges of Cell Culture

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    Blog Post
    ...Mammalian Cells Learn about the Dangers of Using GFP for Protein Localization Resources on Addgene.org...Biotechnology and Biological Sciences Research Council (BBSRC) and Medical Research Council (MRC) require authentic...the amount of money wasted in basic oncological research due to contamination are in the tens or hundreds...lines deposited with them and used by different researchers are free of contamination and match their original...perhaps a working cell bank has been made by a research institution (an excellent practice), or maybe ...Warwick University, gaining extensive cell culture research experience through working for a variety of pharmaceutical..., HeLa cells, and cell culture contamination." Archives of pathology & laboratory medicine 133.9 (2009...
  13. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    Blog Post
    ...used this cassette to knock out genes, knock in a GFP cassette, and introduced site-directed mutations,...selection. In a subcutaneous cancer model, the researchers showed that after 24 hours, the unstabilized ...in nature, these systems have been adapted by researchers for many uses when plasmid retention without ...for long-term experiments. They’ve also helped researchers design and optimize cloning methodologies using...genetic modification of Pichia pastoris." FEMS yeast research 9.4 (2009): 600-609. PubMed PMID: 19416369. Kulakauskas....org Check out our molecular biology reference Search for a plasmid here Find plasmids from our special...
  14. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    ...Lindsey M. Costantini, and Erik L. Snapp. "Superfolder GFP is fluorescent in oxidizing environments when targeted... in Biomedical Research from Albert Einstein College of Medicine. Her doctoral research was conducted ...Figure 1). When behaving properly, FPs enable researchers to investigate the localization and dynamics ...co-mentorship Blossom Damania and Jack Griffith, her research focuses on the replication machinery of Kaposi...secretory pathway."Biochemical and biophysical research communications 430.3 (2013): 1114-1119. Pubmed...
  15. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...Fluorescent Protein Aggregation Catch Up On Your GFP Basics Get Advice on Choosing Your Fluorescent Protein...to monitoring localization; using FP timers, researchers can look at a single image of a cell and understand... production of the initial FP timer in 2000, researchers have realized how problematic aggregation-prone...expression. In a developing embryo, for example, a researcher could determine whether or not newly synthesized...synthetic biology, FP timers might be able to tell researchers whether old or new cells are better at producing...
  16. CRISPR 101: RNA Editing with Cas13

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    Blog Post
    ...which must be stabilized by monomeric superfolded GFP and averages only ~50% RNA knockdown. In initial ... doors for scientific research - how will you use these systems in your research? Cary Valley contributed...developed and potential ways you can use it in your research. RNA editing advantages RNA editing has multiple... forward to the expanded use of RNA editing in research and its potential therapeutic uses We are also...
  17. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...like Luciferase and GFP Resources on Addgene.org Find Plasmids for Your Research     ...not a big issue for cells, but it may confound research results or even kill your cells if your gene of...right tissue-specific promoters as described by researchers at the NIH here.  Note: A. Max Juchheim contributed...
  18. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...mutation can be introduced in a gene, while adding a GFP-tag (Figure 3). Achieving multiple modifications...Molecular Biology. Jake Watson is a postdoctoral researcher in neurophysiology at the MRC Laboratory of Molecular...homologous recombination in vivo." Nucleic acids research 21.15 (1993): 3601. PubMed PMID: 8346047. PubMed...
Showing: 121 - 140 of 204 results