We narrowed to 145 results for: ENDOG

1. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

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   Blog Post

   ...gram negative bacteria periplasm. Efforts to tag endogenous genes for proteins in these compartments using...

2. Plasmids 101: Control Plasmids

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   Blog Post

   ...produce a similar product, but do not target an endogenous gene in your experimental cells. For example,...

3. CRISPR References and Information

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   ... Mendenhall and Myers Mammalian: FLAG tagging endogenous proteins pFETCh_Donor ; additional HDR & gRNA...

4. Fluorescent Proteins 101: When GFP lets you down

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   Blog Post

   ...requiring anaerobic conditions, proteins that bind endogenous fluorescent co-factors can be used. Examples ...

5. Quick Guide to Near-Infrared Fluorescent Proteins

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   Blog Post

   ...Table 1) were optimized for efficient binding of endogenous BV.  When imaging NIR FPs, consider using light...

6. CRISPR 101: Validating Your Genome Edit

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   ...this method, but your edit doesn’t affect any endogenous restriction enzyme sites? Never fear! You can...

7. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

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   Blog Post

   ...CRISPR/Cas mediated HDR) enables the tagging of endogenous genes close to native expression levels. However...

8. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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   ...XFP can be used to amplify the signal. Imaging endogenous fluorescence in live animals only permits visualization...

9. Rett Syndrome

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   ...PI Mecp2 NLucTom Knock-in of NLuc-tdTomato at endogenous MECP2 locus Castaneus MECP2-NLuc-tdTomato mouse...

10. Viral Vectors 101: AAV Variables That Matter

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    ...Astrocyte-selective AAV gene therapy through the endogenous GFAP promoter results in robust transduction ...

11. Antibodies 101: The Basics of Western Blotting

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    Blog Post

    ...demonstrate that the antibody efficiently binds to endogenously expressed protein at physiologically relevant...

12. Recombinase-based State Machines Enable Order-dependent Logic in vivo

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    ... promising strategy for linking RSM inputs to endogenous gene expression (2). Our paper (1) explains ...

13. PiggyBac-ing Through the Genome Editing Field

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    ...genome following donor removal, which alters the endogenous DNA sequence and has been suggested to cause ...

14. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

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    Blog Post

    ...monomeric state is less likely to interfere with endogenous protein structure and function. By generating...

15. Special Delivery: Fluorophore Targeting for FRET Studies

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    Blog Post

    ...2009). However, these reagents can bind numerous endogenous biomolecules in cells nonspecifically, and they...

16. Plasmids for Stem Cell Research

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    ...plasmids that can be used to create cell lines with endogenously-tagged gene variants or browse Addgene’s entire...

17. CRISPR 101: Cytosine Transversion Editors

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    .... They conducted a CRISPRi screen to identify endogenous genes that impact CGBE efficiency. In addition...

18. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

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    ...Adamson, B. (2024). Improving prime editing with an endogenous small RNA-binding protein. Nature, 628(8008),...

19. 15 Hot Plasmids from 2017

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    Blog Post

    ...into TZAP function, Li and colleagues observed endogenous TZAP localization at telomeres and found that...

20. 22 Hot Plasmid Technologies from 2014

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    Blog Post

    ...Sequestration is accomplished by knocking down your endogenous protein of interest and co-expressing its replacement...