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We narrowed to 430 results for: ERG

Showing: 121 - 140 of 430 results

  1. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...delivery methods, but since TEs insert mostly in intergenic regions, gene disruption is less of a concern...to a single location in bacterial genomes, the Sternberg lab developed the INTEGRATE (Insertion of Transposable...transposons-the-jumping-genes-518/   Sandoval-Villegas N, Nurieva W, Amberger M, Ivics Z (2021) Contemporary Transposon Tools...

  2. Important Considerations When Using AAVs

    Type
    Blog Post
    ...has been demonstrated that certain serotypes can undergo axoplasmic transport. These AAVs may move toward...Examining serotypes 1, 8 and 9, not only do they undergo retrograde and anterograde transport, but they ...avoid this rate limiting step and can immediately undergo replication and transcription. However, with scAAV...

  3. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...both methods did a more comprehensive gene list emerge that accurately reflected the scope of genes known... straightforward positive selection of plants undergoing RNAi, and LIIbeta F 1-2 RNAi, which allows assembly...microscopic plants must be visually identified as undergoing RNAi through the fluorescent reporter and manually...

  4. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...substrate, luciferin, which releases energy as a photon. The photon energy is transferred to the acceptor fluorophore...pcDNA3.1+-pHlash, that use Bioluminescence Resonance Energy Transfer (BRET) were developed by the Johnson Lab...fusion protein (referred to as dCas9-VPR) had a synergistic effect on target gene activation. The novel dCas9...rely on the principle of bioluminescence resonance energy transfer (BRET), whereby photons emitted by a Renilla...

  6. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...permeabilization with detergents such as triton-x, saponin, or tween. Take care when using strong detergents like triton-x...resources References: Bonner WA, Hulett HR, Sweet RG, Herzenberg LA (1972) Fluorescence Activated Cell Sorting...

  9. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TCATGGTGCAGCCGTTACtggacagccgcgttccCTACGGACGCCTCCAGCA Purinergic receptor Y2 TAL3348 & TAL3349 TTCAACGCATCAGTGAATgttcaaaaccacagttCTGCACACAGCTGCAGCA...

  10. Rett Syndrome

    Type
    Collection
    ...1147. (Link opens in a new window) PMID: 17289941 Hagberg et al. 1983. A progressive syndrome of autism, ...

  11. Retrograde AAV viral preps

    Type
    Collection
    ...standard viral production , these viral vectors undergo quality control, including AAV titering by SYBR...

  12. Caltech Systemic Capsids

    Type
    Collection
    ...standard viral production , these viral vectors undergo quality control, including AAV titering by probe-based...

  14. Fueled by Coffee at #SfN14

    Type
    Blog Post
    ...accounts for many, many lattes and espressos. The energy boost is well needed to keep up with all the awesome...great opportunity – so if you’re running out of energy, then it’s time for a coffee run! ...

  15. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    ...Preparing electrocompetent cells (protocol)  Untergasser Lab Preparation of chemically competent cells... (protocol)  Electroporation (protocol)  Untergasser Lab Transformation of chemically competent cells...

  17. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    ...effort was led by two amazing postdocs, Kristin Krukenberg and Jessica Polka from Harvard Medical School...Bourne charged the audience to use their useful energy and to choose institutions with allies engaged ...

  18. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    ...spondylarthritis, using Bioluminescence Resonance Energy Transfer (BRET) technique, and plasmids to study...and worked around the world, carrying out her undergraduate work at the University of London and the University...

  19. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ...being opened in the pathogen’s cell membrane (Dunkelberger & Song, 2010). As cells cannot survive when ...):443-6. doi: 10.1038/74704. PMID: 10742152. Dunkelberger, J., Song, WC. Complement and its role in innate...

  20. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...understand about Dopamine? DA is produced by specific dopaminergic neurons in a brain region called the substantia... Report endogenous DA release from a single dopaminergic fiber in mouse brain slices Demonstrate DA detection...
Showing: 121 - 140 of 430 results