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We narrowed to 856 results for: HA

Showing: 121 - 140 of 856 results

  1. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...and divide along with the endogenous DNA. AAV also has low immunogenicity, meaning it does not induce the...conducive to targeting all cell types. Altogether, AAV has many excellent features for human gene therapy and...administration, such as in mouse experiments. AAV also has multiple serotypes (12 naturally occurring and more...constraints are an issue. Adenovirus Adenovirus has a linear, double stranded DNA genome which is non-enveloped...cancer in hamsters, although no cancer-connection has been identified in humans to date. Over fifty serotypes...have been identified so far! Adenovirus generally has high immunogenicity, but some strains have been engineered...other viruses discussed in this article, adenovirus has the highest maximum titer, a plus for production ...

  2. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type
    Blog Post
    ...lab at the Massachusetts Institute of Technology has been “working on new experimental and theoretical...and medicine. As they’ve gone along, Voigt’s group has also been assembling the toolbox needed for anyone...CRISPR circuitry, it can slow their growth. Something has to be done about that.  The second problem is fundamental...this now, we have ideas, but so far nothing that has worked. I don’t have the solution. Our paper shows...transcriptional and translational activity a cell has and control how much it taxes the resources of a ...antibodies. But we are really just using what biology has given us. Wood is a complicated material and we can...can access it, but we are stuck with what biology has given us. Over the next 100 years, we are really ...

  3. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...CRISPR has greatly enhanced the ability of scientists to make genomic alterations, bringing about a revolution...inserting a custom gRNA.  While this methodology has proven to be incredibly valuable to scientists, there... used to deliver a Cas9-gRNA RNP. This advantage has allowed scientists to perform in vitro and in vivo...to the use of this technique in cell culture, it has also been applied to genome editing of mouse zygotes...the early stages of development. David Liu’s Lab has demonstrated the use of cationic lipid-mediated methods...et al. 2015). A more targeted approach using RNPs has recently been described in vitro, with the hope of..., as an alternative to plasmid based techniques, has been an area of intense study. Two methods to accomplish...

  4. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...CRISPR/Cas9 has made it easier than ever to make precise, targeted genome modifications. Cas9 has been modified...which one of Cas9’s two critical enzymatic residues has been converted to an alanine (D10A or H840A), changing... for your experiment, ensure your sgRNA sequence has little homology to other regions of the genome and...looser PAM requirements compared to SpCas9. xCas9 has a PAM requirement of NG, GAA, or GAT, making it a...SpG variant, discovered by the Kleinstiver group has a PAM of NGN (Walton et al., 2020). Further, this...reverse transcriptase, and a nCas9. While this system has been shown to be less efficient than base editing...directly fusing the cargo to dCas9.  This technique has been exploited to localize factors such as transcriptional...

  5. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...inflammation and degeneration has been reported (Muraine et al., 2020). AAV8 AAV8 has high transduction efficiency...nervous system (CNS) (Wu, Asokan, et al., 2006). AAV1 has also been found to transduce skeletal muscle, cardiac... the most common choice for AAV pseudotyping. It has broad tissue tropism, including liver, muscle, lung...neural connections. Although derived from AAV2, AAVrg has not been thoroughly studied in contexts outside the...generally the best choice for targeting astroglia. It has additionally been reported to have tropism for liver...administration (Halbert et al., 2001). It additionally has tropism for cardiac and liver cells (Issa et al.,...applications targeting liver tissue. In addition, it has been shown to transduce skeletal muscle, cardiac,...

  6. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type
    Blog Post
    ...give me a brief history of optogenetics? How long has this idea been around? Boyden: Optogenetics is a ... silencers of neural activity. Since then, there has been a steady stream of papers describing new photosynthetic...against each other. Getting two-color stimulation has been a holy grail of the field. The problem is that...is fundamental understanding of the brain, which has humanistic and philosophical implications. As an ...Addgene over the years, many of them very popular. Why has it been so important to you to distribute these tools...as possible? Boyden: I think because neuroscience has so many different goals: to understand how a mouse...

  7. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type
    Blog Post
    ... to be activated by the DREADD actuator after it has been administered. Although we found a larger proportion...DREADD to the plasma membrane. The haemagglutinin (HA) tag is a small 9 amino acid epitope of the haemagglutinin...al., 1988). Thus, we injected AAVs carrying hM4Di-HA in the brains of mice and monkeys. Surprisingly, ... a big difference. Replacing the mCherry with the HA tag resulted in abundant expression of hM4Di in plasma... where the fluorescence is not needed, the use of HA and other short epitope tags (Kimple et al., 2013...used to reveal the hM4Di coupled to mCherry (top) or HA tag (bottom). The images show examples of dendrites... to the plasma membrane. Note that the use of the HA tag significantly improves the transport of hM4Di...

  8. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...both a fundamental driver of these principles and has emerged as a byproduct of connectivity. Between 2012...integrated with most of the tools noted above, and has a modern API. Integrations with additional tools ..., so it’s possible to re-export a protocol which has been edited back to protocols.io. Thus, RSpace becomes...Figshare. Any ELN that facilitates reproducibility has to make it easy to export data from the ELN directly... these five general purpose repositories. RSpace has been integrated with two of the five: Figshare and...additional metadata in the repository after the deposit has been made. It’s possible to associate the depositor...the repository. The ability to deposit data that has already been structured in folders or notebooks in...

  9. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    ... a family affair back in 2004. As Addgene has grown, it has taken effort to continue the tradition and...goofing off, the company culture here at Addgene has remained paramount. Candidates who interview at Addgene...testament to the familial aspect of Addgene. As our size has drastically increased, it would have been a tight..., even for an event where there is food and beer. Ha! We had over 60 people join the fun - including a... or binging a new Netflix series. The thing that has made all the difference here at Addgene is the emphasis...and a cooler. Chances are one of your colleagues has a frisbee at least. Use it! 8. Organize If you’re...there is something fun happening! Addgene actually has chimes outside of our break room. A ring of the chimes...

  10. Plasmids 101: Protein tags

    Type
    Blog Post
    ...which a good antibody has not been developed (and just because your protein has a commercially available...localization, or protease protection. Thus far Plasmids 101 has covered GFP and its related fluorescent proteins,...affinity tag to it. The FLAG, hemaglutinin antigen (HA), and c-myc tags have been the workhorses of the ...Purification Good for antibody-based purification; has inherent enterokinase cleavage site GST Large Protein...against proteolysis, but may reduce solubililty HA YPYDVPDYA or YAYDVPDYA or YDVPDYASL  1.1 Affinity... a moment), and 2 ProtA IgG-binding domains. TAP has since come to encompass several other tag combinations...necessarily want them to stick around after that purpose has been served. At this point, proteases can be your...

  11. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...EGFP into pAG Lenti CMV N-HA Puro (Addgene #236079) to create pAG Lenti CMV HA-EGFP Puro (Addgene #236081...by the Loren Looger and Kaspar Podgorski Labs now has a new version: iGluSnFR4, developed by the GENIE ...we're excited to share our very own set of empty, HA-, and EGFP-expressing lentiviral backbones designed...Choose untagged or add a versatile N- or C-terminal HA tag. Selectable and stable: A puromycin resistance...Anti-GFP [N86/38.1R] (Addgene #180084), 0.01 µg/mL Anti-HA [12CA5] - Chimeric (Addgene #198003)) and Goat anti-Mouse... loci in the genome. The modified transposon RNA has untranslated regions (U, blue) on the outside of ...12 kb — with efficiencies between 5% and 11%. It has higher efficiency and lower indel formation than ...

  12. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    ...Alternatively, you can select a target sequence which has already been shown to work in CRISPR experiments....experiments. Addgene’s dedication to resource sharing has led us to develop a searchable and sortable datatable ... to the fact that every gRNA listed in the table has been used effectively in a published article. This...target sequence, and model system. Even if a gRNA has been demonstrated to work in your system, it is worth...that locus. The data in the validated gRNA table has been derived from scientists’ submissions, either...

  13. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    ...artifacts, it has better folding and maturation kinetics than other dark fluorescent proteins, it has good signal...Technology (KAIST) and Institute for Basic Science (IBS) has developed another useful optogenetic tool that enables...facilitate future work with this goal, the Barrick lab has deposited the pDCAF3-IS5 plasmid containing a synthetic...operon originates from Pseudomonas putida CBB5 and has been tailored for expression in E. coli. This operon... contributed by Christina Mork The Murakoshi lab has developed two new FLIM-FRET acceptors, ShadowG, a... due to its very low quantum efficiency. ShadowY has a lower quantum yield and higher absorption than ...

  14. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    ... world has battled the COVID-19 pandemic over the past few months, the scientific community has seen a...research into this disease. A key reason this research has been able to progress so rapidly is scientific sharing...is applicable to researchers worldwide. “COVID-19 has spread incredibly fast and without any notion of ...COVID-19 Resources Canada site. To date, the portal has recruited 3,218 Canadian volunteers, and features...

  15. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    ...story, to get scientists excited about what Addgene has to offer, and to help scientists find what they need...homepage. Blugene, our trusted friend and mascot, has undergone a make-over as well. While in the course...Blugene needed the same thoughtful treatment. Blugene has been with us since 2011, and while you may have seen...and sharing.  One big feature upgrade? Blugene now has a full expression. You’ll mostly see a friendly smile...an experiment doesn’t go as planned. Blugene also has full arms so that they can hold and hug items closer...

  16. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ... Even though GFP has been so thoroughly characterized, it turns out this protein has a few more secrets...photoconvert GFP from green to RED! This simple technique has been shown to work in plant, Drosophila and mammalian...cases, however, a GFP-tagged cell line or organism has already been made, and it would be expensive and ...is great news for microscopy enthusiasts! Addgene has empty backbones containing GFP variants used by Sattarzadeh...

  17. Make a Splash: Notions of Scientific Impact Are Evolving

    Type
    Blog Post
    ... even society. One obvious way to do that is and has been to publish in journals with a high “impact factor...cited articles appearing in lower-prestige journals has risen steadily from 1995 to 2013. Considering citations...online access on scientific impact Partly, the story has to do with online accessibility. It is now about ...among the most cited. And, as scientific discourse has increasingly moved online, surely times have changed...

  20. Lentiviral Vector Guide

    Type
    Guide
    ...Retroviridae family. Infection with lentiviruses has a long incubation period that eventually leads to... genes, mutations, or treatments into cells, and has been widely used in the research community for decades...VSV-G is by far the most common envelope gene, as it has a wide range of infectivity across many cell types...their stable expression and wide tropism. Addgene has an extensive collection of lentiviral CRISPR libraries...
Showing: 121 - 140 of 856 results