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We narrowed to 873 results for: LIC

Showing: 121 - 140 of 873 results

  1. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert PI Publication Return to top ...top Plant ID Plasmid Gene/Insert PI Publication Return to top CRISPR Resources Addgene has a large selection...

  2. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert...Insert Promoter Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter Selectable...Selectable Marker PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids...

  3. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert Selectable Marker PI Publication Return...insertions — meaning they may not be suitable for applications where orientation of the insert is important...

  4. Deep Dive: qPCR

    Type
    Blog Post
    ...Background noise Probe Allelic specificity Can be multiplexed Single amplicon validation technique ... and dsDNA. This is not true for all amplicons; if your amplicon is AT-rich or has a secondary structure...understanding and technical proficiency with common applications - now let’s dive right in! You know qPCR is...as it solves, so understanding and using the application correctly is imperative. Picking your chemistry...hairpin design of probes/primers often offer higher allelic specificity, due to the need for the target:probe...Bustin, 2017 for a more in-depth review.)  Your amplicon should be between 70-200 bp long With ΔΔCT analysis... intensity should smoothly increase as the DNA amplicon transitions from single-stranded to double-stranded...

  5. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    ...offer export license applications for VSV and SARS-COV-1 plasmids. Export control license process    An...excited to announce we now offer an export license application for rabies materials! Some of the materials...cannot expedite the application process.  More information about export control licenses is available through...the USA, where Addgene is located.  The export license, if granted, allows us to ship rabies plasmids ...  An export control license, once approved, allows Addgene to ship plasmids or viral vectors containing...will need to provide a brief description of the applicable research project when purchasing export-controlled...export-controlled materials. Addgene will then apply for the license for distribution to the requester’s organization...

  6. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...TCGATGAATTCGAGCTCG–3' ID Plasmid Selectable Marker Tags Publication FP Overexpression Plasmids for constitutive overexpression...marker. ID Plasmid Gene/Insert Selectable Marker Publication Additional Resources Addgene Kits MoClo-YTK Yeast.... L., von Wirén, N., Frommer, W. B. (2007). A cytosolic trans-activation domain essential for ammonium...

  7. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...Protocols & Resources Contents of Kit Original Publication Oligomerized pool engineering (OPEN): an 'open-source... in the Materials and Methods of your future publications. For your Materials and Methods section: "The...The plasmid kit used in this publication was a gift from Keith Joung (Addgene kit # 1000000013)" For your...

  8. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ...  Limitations of the in silico approach The in silico approach cannot replicate the complexity of antigen... risk of an antibody.  In silico prediction of adverse reactions In silico assessment uses computer modeling...from the in silico assessments, as well as analyze the contributing factors that in silico prediction ... drugs are still immunogenic, meaning that they elicit an immune response from the body. Several factors...responses vary depending on the person. However, in silico and in vitro assessment of immunogenicity can help...drug epitopes likely to be presented by T cells to elicit an immune response. T cell epitopes are found in... evaluate the immunogenicity of therapeutics in silico. These algorithms are trained on vast amounts of...

  9. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...Purchase and the applicable material transfer agreements (MTAs) and limited use label licenses (LULLs). Titer...contact you directly regarding these charges, if applicable....

  10. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    ...received 116,224 employment based green card applications and approved 91,023 during the first 3 quarters... development, business development, consulting, policy, big data and the list goes on and on. As you think...options discussed in this post benefit from the applicant thinking about the process early, keeping good...occupation" as requiring theoretical and practical application of a body of highly specialized knowledge in ...bachelor's degree or its equivalent and state licensure, if required to practice in that field. H-1B work-authorization...Hire American" Executive Order which sets broad policy intentions directing federal agencies to propose... April 1 deadline for companies to submit an application on their behalf.  If an individual obtains a ...

  11. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...that in a bit! Viral genomes and replication When it comes to replication, viruses tend to march to the ...methods of replication. In fact, an entire classification scheme, known as the Baltimore replication classes...developed around how they replicate (Figure 2). Figure 2: Baltimore replication classes. Image credit: ...an intermediary DNA replication step between DNA and mRNA. DNA genome replication can involve an RNA or...cell. The cell then uses its own machinery to replicate the viral genome and proteins. This is, for the...before being translated into proteins. RNA viruses replicate their genomes using a variety of RNA or DNA intermediaries... bacteria and eukaryotes, which use a binary replication process whereby one cell copies itself and “splits...

  12. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...are used across species: replication, selection, and expression. Replication It’s usually not enough to...chromosome has a single origin of replication (ori), where DNA replication begins, so a bacterial plasmid...Eukaryotes are a bit more complicated. Yeast vectors may contain an autonomously replicating sequence (ARS), a...fast-growing populations of cells, the vector needs to replicate alongside its host, or it will be lost from the...compatible ori that will be recognized by its host’s replication machinery. A shuttle vector designed for unrelated...experiment. Yeast shuttle vectors have these replication sequences for their yeast targets as well as ...particularly mammalian cells like neurons, may not replicate during the course of your experiment, meaning ...

  13. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...two types of replicate controls: technical and biological. In general, technical replicates can be thought... employ transfection, negative, positive, and replicate controls.  Transfection controls: empty vector...the interrogation of the independent variable. Replicate controls: technical and biological Reliable results...derived from one source. Conversely, biological replicates ARE independent and can be thought of as "reproducibility...reproducibility controls". Biological replicates are what makes up your sample size (aka your n value)"... provide more in depth information. Technical replicates An example of a technical control is the transfection...plasmid from the same aliquot/preparation. The replicate control isn’t measuring or assessing reproducibility...

  14. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type
    Blog Post
    ...after credible attempts to replicate major findings could not be replicated by other researchers.There...published research is difficult to replicate.  Why is replicating previous research findings so difficult...can be difficult to replicate. Unclear methods Until someone attempts to replicate the results of another...positives in original studies and false negatives in replications are unavoidable, but can be minimized with high...obtaining a different result after conducting a replication study. An important difference between the original... methods, QRPs, and small samples, then when replications do not come out the same as an original research...data, materials, and code Only when you try to replicate the work of other researchers (or your own from...

  15. AAV for Neuronal Tracing

    Type
    Collection
    ...which is not required for the transcription or replication of the genome within infected cells, but is required... cells that lack the G protein, the virus can replicate but the newly formed viruses are unable to infect...starter cell via the EnvA receptor. RABV is able to replicate in the starter cell using the G-protein, which...

  16. Viral Vector Guides and Plasmids

    Type
    Collection
    ...strong immunogenic response, useful for clinical applications as vaccines. Selecting a Viral Vector Type Each...been carefully engineered for safety and are replication-incompetent. Specific genes have been mutated...mutated or deleted to prevent uncontrolled viral replication, and the required genes are divided in the multi-plasmid...

  17. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ...someone has replicated it precisely. You can find a great discussion of different types of replication in Nosek...perfect story for a “high impact” publication can lead to the publication of rare flukes showing positive...adoption of policies that promote reproducible publishing practices. One example is the publication of peer...Wednesday we worked with the Harvard GSAS Science Policy Group to organize a Minisymposium on Reproducibility... out on the panel, irreproducibility undermines public trust in science and research. Nonetheless, I’d...difficult to publish so-called “negative” results that conflict with our original hypotheses. Looking for novelty...these positive results will be published. The publication record will then show that the tested hypothesis...

  18. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...recombination. Yeast Replicating plasmids (YRp):  These vectors contain an Autonomously Replicating Sequence (ARS...mammalian vectors, will catch you up on the core replication and resistance features of yeast vectors and ...includes but is not limited to: rapid growth, ease of replica plating and mutant isolation, a well-defined genetic...vectors must contain a yeast-specific origin of replication (ORI) and a means of selection in yeast cells...antibiotic selection markers.  The yeast origin of replication (Please note: This first section primarily pertains...ve also noted some features required for the replication of fission yeast, Schizosaccharomyces pombe, ...within a yeast vector determine how the plasmid is replicated and maintained within the yeast cell. These elements...

  19. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ... to verify there’s no genomic integration Replicating EBNA1 episome Non-integrating Medium Medium ...creates heterogeneous iPSC cell lines, which can complicate comparisons made between lines. Incomplete silencing...Retroviruses are not considered safe for clinical applications.     Lentiviral: Lentiviral vectors infect dividing...unavoidable heterogeneity between clones. This can complicate comparisons made between samples, i.e. transgene...delivery is not considered safe for clinical applications. Excisable Cre-lox lentiviral vectors: This...is still not considered safe for therapeutic applications. piggyBac (PB) transposon: The PB transposon...express reprogramming factors for therapeutic applications. Sendai viral vectors: Sendai virus is a single...

  20. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...Institute for Cell Science has produced the first publicly available collection (Link opens in a new window...Proliferating cell nuclear antigen (PCNA) DNA replication foci IPSC cell lines can be ordered from the ...: 28814507 (Link opens in a new window) More publications from the Allen Institute for Cell Science (Link...
Showing: 121 - 140 of 873 results