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Showing: 121 - 140 of 815 results

  1. Viral Vectors

    Type
    Collection
    ...of certain genes that are required for viral replication. Thus, as a safety feature, viruses used in research...Instead, the genes that are required for viral replication are usually divided among many plasmids, such...

  2. Church Lab CRISPR Plasmids

    Type
    Collection
    ...superior for less favorable protospacers and applications requiring particularly tight binding. In these...advisable to use the consensus sequence. See the publication for details. A protocol for synthesizing gRNAs...

  3. Ras Pathway

    Type
    Collection
    ...-25% of all human cancers. Ras Pathway Plasmids Click on a name to find available plasmids for the gene... Research Return to top Ras Pathway - Gene List Click on a name to find available plasmids for the gene...

  4. Neurodegeneration Research Collection

    Type
    Collection
    ...loci seem to be associated with the disease and implicate a wide range of cellular processes. The vast majority...Rep. 2023 Aug 30. Enhance differentiation to mesencephalic dopaminergic (mesDA) neurons using gene knockout...

  5. Caltech Systemic Capsids

    Type
    Collection
    ...Information When using the Caltech serotypes in future publications, please acknowledge Viviana Gradinaru and cite....PHP.N with GRE or transgenic animal For PNS applications in rodents and NHPs: Broad tropism - AAV-MaCPNS2...

  8. Centrifugation

    Type
    Protocol
    ...according to speed and time (and temperature, if applicable) listed in your protocol. *Pro-Tip* Spin speed...

  9. General Transfection

    Type
    Protocol
    ...DNA. Add the diluted PEI dropwise while gently flicking the diluted DNA tube. Incubate the mixture 15–...

  11. Gibson Assembly Protocol

    Type
    Protocol
    ...Link opens in a new window) . Gibson Assembly® is licensed to New England Biolabs by TelesisBio Gibson DG...

  12. Immunocytochemistry

    Type
    Protocol
    ...as methanol or acetone may be better for some applications. Remove the paraformaldehyde and follow your...

  13. Colony Formation Titering Assay

    Type
    Protocol
    ...lentiviral preparation allows you to control the multiplicity of infection (MOI) in downstream studies. This...

  16. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...pH2B_mCherry_IRES_puro2 Chromatin H2B mCherry Daniel Gerlich 25999 LV-GFP Chromatin H2B GFP Elaine Fuchs 11680...Reticulum ER retention signal E2-Crimson Benjamin Glick 36204 pmTurquoise2-ER Endoplasmic Reticulum ER retention...

  18. AAV ddPCR Titration

    Type
    Protocol
    ...quantification. This protocol was modified from the publication Lock et al. (2014) (Link opens in a new window...

  19. AAV Production in HEK293 Cells

    Type
    Protocol
    ... layers are covered with media. 293T cells are delicate and detach very easily - media should always be...

  20. Lentivirus ddPCR Titration

    Type
    Protocol
    ... sample. This protocol was modified from the publication Wang et al. (2018) . Before Starting Thaw the...
Showing: 121 - 140 of 815 results