...doi.org/10.1016/j.cell.2013.08.021 Waters CA, Strande NT, Pryor JM, Strom CN, Mieczkowski P, Burkhalter MD...regardless of end structure, and dictate the major events of the pathway: Broken ends are recognized by ... probably accounting for less than 5% of repair events. However, the products of accurate repair are easily...Yan et al., 2020). NHEJ can also be engaged by variants of the canonical Cas9 approach. A pair of CRISPR...

...introduce the small wrmScarlet11 which is less than 60 nt in length to tag the target protein. The team validated... without the constraints of antibody labeling. This opens the door to in vivo experiments such as tracking...composed of two FP fragments that are not fluorescent on their own. When the two fragments come together, ...

...default nucleotide database “Nucleotide collection (nr/nt)” as it contains a composite of GenBank, EMBL, DDBJ...of the different BLAST programs, databases, and elements of the BLAST search pages. At Addgene, we use ...not as fast as megablast, but can return longer alignments to compare with your sequencing trace file. Unlike...be your optimal choice. For difficult sequence alignments that BLAST is unable to handle, Clustal is our...frequent choice for pairwise or multiple sequence alignments of nucleotide or protein sequences. We also use... to confirm your plasmid sequencing results or comments on our suggestions? Share your thoughts here to...

...addgene.org to help us improve our resources! Acknowledgments This project is supported by a NIH BRAIN Initiative...

...biology plasmids related to metabolic pathways and components. Examples Include: Biofuels Natural Product Pathways...

...created a number of plasmids for expression of variants of LRRK2 , SNCA , and other proteins of interest...

...scientists can design de novo systems using modular components or take advantage of existing GFP-lines for cell-specific...

...both naturally ocurring and synthetic regulatory elements, pre-assembled genetic circuits, and higher level...

...create cell lines with endogenously-tagged gene variants or to differentiate human iPSCs into other cell...

...will produce a catalog of the impact of genomic variants on genome function and phenotypes. Plasmids The...

... Krüppel-like zinc fingers which bind to CACCC elements or GC-rich regions on DNA to mediate transcriptional...

...research to discover cures and more effective treatments for carcinoid, pancreatic, and related neuroendocrine...

... TALengineering Reagents TALE Repeat Plasmids Individual REAL TALE Repeat...

... for expression in mammalian systems, bacteria, plants, and yeast. Mammalian ID Plasmid Gene/Insert Promoter...

...drive selective expression in the brain - Simpson Reagents for improved expression of recombinant phosphoproteins...

...expression in mammalian systems, bacteria, Drosophila, plants, and yeast. Mammalian ID Plasmid Gene/Insert Promoter...

...first step for many different kinds of important experiments. In an effort to speed up research of SARS-CoV...

... expression in mammalian systems, bacteria, and plants. Mammalian ID Plasmid Gene/Insert Promoter Selectable...

...mammalian cell types. AAV is used in a wide range of experiments, both in vitro and in vivo. It has low immunogenicity...

...expression in mammalian systems, bacteria, Drosophila, plants, C. elegans, yeast, zebrafish, and Xenopus. Mammalian...