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We narrowed to 497 results for: RAP

Showing: 121 - 140 of 497 results

  1. An Introduction to Adenovirus

    Type
    Blog Post
    ... Adenovirus Vectors for Gene Therapy, Vaccination and Cancer Gene Therapy. Curr Gene Ther, 13(6), 421–...was first isolated from human adenoid tissue and rapidly caused the death of adenoid cells in vitro (Rowe...oncolytic virus in addition to standard uses in gene therapy and in basic research, like other viral vectors...novo viral gene expression: Implications for gene therapy. Proc Natl Acad Sci U S A, 95(19), 11377. https...

  2. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...Reference CBE SpCas9 rAPOBEC1 NGG 6 SpCas9 Target-AID (PmCDA1) NGG 5 SpCas9-VQR rAPOBEC1 NGA 8 SaCas9-... plant systems. Target-AID based (CBE-2)-  and rAPOBEC based (CBE-1)-  CBEs are successful base editors...rice, tomato, wheat, and potato. Target-AID and rAPOBEC based CBEs are known to deaminate cytosine placed...SaCas9-KKH rAPOBEC1 NNNRRT 8 ABE SpCas9 TadA-TadA* NGG 9-12 SpCas9-VQR TadA-TadA* NGA 8 SaCas9 TadA-TadA...

  3. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...valuable tool for microglia research and broader therapeutic applications.  Find microglia-targeting AAV plasmids...10.1101/2023.12.12.571321.  Revolutionizing Gene Therapy: The REVeRT Dual AAV Vector System By Vaibhav Kawde... typically associated with viral vector-based therapies. REVeRT supports advanced gene expression regulation...mechanisms of delivery and the potential for therapeutic success. Find REVeRT plasmids here! Riedmayr...dual AAV vectors for (epi)genome editing and gene therapy. Nat Commun., 14(1), 6578. https://doi.org/10.1038...

  4. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type
    Blog Post
    ...opened up vast possibilities for the clinical and therapeutic applications of iPSCs. The generation of human...obtain. These can then be used to perform cell therapy and to model diseases in vitro. Find stem cell ... The phenomenon has been recorded in past gene therapy trials and remains a safety concern in the clinical... cell proliferation in two patients after gene therapy for SCID-X1. Science, 2003. 302(5644): p. 415-9...Ehrhardt, A., et al., Episomal vectors for gene therapy. Curr Gene Ther, 2008. 8(3): p. 147-61. PubMed ...

  5. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...genome-containing (Figure 1).    Figure 1: Electron micrograph of an Addgene AAV viral vector preparation after... and improved alternative methods." Human Gene Therapy, Part B: Methods 23.1 (2011): 1-7. PubMed PMID:...can limit production of recombinant AAV-2." Gene therapy 6.7 (1999). PubMed PMID: 10455443. 3. Hindson, ...type 2 Reference Standard Material." Human gene therapy 21.10 (2010): 1273-1285. PubMed PMID: 20486768.... infectivity of recombinant AAV vectors." Gene therapy 17.7 (2010): 872-879. PubMed PMID: 20336156. PubMed...

  6. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...aliquots, and stored at -80°C. Some reports suggest rapidly freezing virus in a dry ice/ethanol bath or liquid...processing of oncoretroviral and lentiviral gene therapy vectors.” Biotechnology Advances 24.3 (2006). 321...lentiviral and retroviral vectors.” Human Gene Therapy Clinical Development 25.3 (2014). 175-85. Pubmed...lentiviral vectors through pseudotyping.” Current Gene Therapy 5.4 (2005). 387-98. Pubmed PMID: 16101513. PubMed...predicting gene transfer events.” Genetic Vaccines and Therapy 2.1 (2004) Pubmed PMID: 15291957. PubMed Central...

  7. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...inserted downstream of a functional promoter (gene traps). The resultant gene targeting system could be used...the efficiency of homologous integration via gene trap enrichment, whereas pAAV-TK-Acceptor has a conventional... in human pluripotent stem cells." Molecular Therapy20.2 (2012): 329-338. PubMed PMID: 22108859. PubMed...DSB-mediated gene editing technologies." Current gene therapy 16.3 (2016): 207-219. PubMed PMID: 27280971. 10...nucleases with adeno-associated viral vectors for therapeutic gene editing." Precision Medicine, CRISPR, and...

  8. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...and W describe the shape of a histogram, which graphically represents the time and intensity of the cell...the detected photons can be represented through a graph of photocurrent vs. time. The resulting histograms...expected size. The strong signals close to the graph’s origin mainly stem from cell debris and other small...wondering which second measurement to choose for graphically displaying your events. You are free to choose...T killer cells but not dendritic cells. Using a graph displaying both stains, I can quickly separate T...

  9. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...coming of age. Molecular Therapy : the Journal of the American Society of Gene Therapy, 17(8), 1316–1332. https...clinical settings, as evidenced by retroviral gene therapy trials for X-linked severe combined immunodeficiency...lentiviral vectors: progress and applications. Gene Therapy, 17(2), 150–157. https://doi.org/10.1038/gt.2009.135...mutations causes leukemogenesis following gene therapy of SCID-X1 patients. The Journal of Clinical Investigation...

  10. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...opening and allowing positively charged ions to rapidly pass through the cell membrane and initiate an ... their experiments. Some tools turn on and off rapidly with light to control precise timing of the perturbation...activation kinetics Some channels turn on and off very rapidly in response to a single light pulse whereas others...responses (Gunaydin et al., 2010). This can affect how rapidly you can activate or inhibit a cell in succession...considering for your experiment, note that you cannot extrapolate results from one cell type to another, as they...

  11. Michael Koeris' Journey from Grad Student to Entrepreneur: The Story of Sample 6

    Type
    Blog Post
    ...not right for the development of phage-based therapeutics. After a brief stint at the venture capital ...Novophage's focus. The company went from developing therapeutics to developing phage-based tools for detecting...develop tests for food products as opposed to therapeutics 15:15 - 16:26 : Means for testing for pathogens...for Dr. Koeris to transition from developing therapeutics to developing a diagnostic? 36:53 - 38:00 : ...

  12. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...fragment with FKBP rapamycin binding domain of mTor (Cas9(N)-FRB-NES) resulting in a rapamycin-inducible Cas9...Addgene: Rapamycin-inducible Cas9 sets (Addgene plasmids 62883 &62884; 62885 & 62886) Rapamycin-inducible...Cas9 for genome editing. Without rapamycin treatment, the Cas9(N)-FRB-NES fragment is actively shuttled...to the nuclear export sequence. Treatment with rapamycin induces Cas9(N)-FRB-NES and Cas9(C)-FKBP-2xNLS...splicing   The Gideon Dreyfuss lab has developed rapid-response luciferase (firefly P. pyralis) reporter...e.g. fluorescence recovery after photobleaching [FRAP]) for studying membrane dynamics, for monitoring...

  13. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type
    Blog Post
    ... Figure 2: A bacteriograph of Michelangelo's "The Creation of Adam." This bacteriograph was created using...Mustafa Khammash's lab at ETH Zürich. “Instead of a graphical abstract, it’s a musical abstract,” says Di Ventura...). These images are based on the concept of bacteriographs created about a decade ago. To create these...

  14. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    ...advancement of SARS-CoV-2 research because they provide rapid access to validated reagents, especially during ...nonprofit research institution, has been able to rapidly propagate this strain, making it available to scientists...provides. Repositories around the world facilitate the rapid pace of SARS-CoV-2 research by removing logistic...hurdles to continue making materials available rapidly to scientists (Nature Biotechnology 2020). The ...

  15. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    ...your writing habit into beautiful graphs! I like to look at these graphs when I need that extra push to ...to stick with my writing routine. The graph of which days of the week you most often completed your habit...flagging passive voice, overuse of adverbs, and paragraphs with many hard-to-read sentences.  Grammarly ...

  16. dTAG - You're it!

    Type
    Blog Post
    ...advantages over pharmacological inhibitors including rapidly depleting a protein-of-interest, increasing target...system as we can use it to evaluate the effects of rapid and selective degradation of protein targets in ...machinery through binding with cereblon, leading to rapid target degradation (Figure 1). Plasmids: We generated...tagged, the dTAG lentiviral plasmids can serve as a rapid means of comparing functionality of N- and C-terminal...

  17. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type
    Blog Post
    ...levels? Until recently, no such tool existed for rapid, customizable protein degradation. Enter TRIM-Away...antibody targeting the protein of interest led to rapid, targeted degradation that is dependent on proteasome...targeting of mTOR producing similar results to rapamycin inhibition. To assess TRIM21 toxicity, Clift et...FIRE–Cas9), combines these approaches to enable rapid and reversible recruitment of chromatin complexes...

  18. All in a Twist: dsRNA

    Type
    Blog Post
    ...mRNA. These RNA tools have been used in clinical therapy to target pathogenic proteins, as well as in the... In the same vein, genotoxic stress, such as chemotherapy treatment or ionizing radiation, can also result...responses are also a concern when it comes to RNAi therapeutic delivery. Thus, balancing the amount of siRNA...just for DNA. Untwisting RNA is important for therapeutic delivery, viruses, human disease, and much more...

  19. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ...contributed by Jennifer Tsang AAVs are a great gene therapy delivery mechanism, but their small packaging capacity...tested the base editors in vivo and achieved therapeutically relevant editing efficiencies in the mouse ...comparable to those currently used in human gene therapy trials. They further tested the base editors by...Niemann-Pick disease type C, directly demonstrating the therapeutic potential for the split base editors. Find the...

  20. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    ... only for translational research, such as gene therapy, but also basic research. To improve cell-type ... with high rates of labeling are ideal of gene therapy applications, promoters that produce sparse labeling...basic as well as translational research for gene therapy. The Roska lab hopes to design improved versions...blog Read more about using AAV for retinal gene therapy Find more about precise neuronal targeting using...
Showing: 121 - 140 of 497 results