We narrowed to 982 results for: Ran;

...identified by depositors and/or when BRAIN Initiative grants are noted in publications associated with Addgene...-GfaABC1D-mCherry-hPMCA2w/b Expresses a plasma membrane calcium pump hPMCA2w/b Baljit Khakh 112000-AAV1...flip-excision switch, for AAV production or direct transfection, slow variant Kaspar Podgorski 135421-AAV1 pAAV-syn-FLEX-axon-jYCaMP1s...flip-excision switch, for AAV production or direct transfection, slow variant Kaspar Podgorski 135424-AAV1 pAAV-syn-jYCaMP1s...Synapsin1 promoter, for AAV production or direct transfection, slow variant Kaspar Podgorski 135630-AAV1 pAAV-S5E2...interneurons. For use in combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent... expressed under the mammalian promoter (EF1a) Francois St-Pierre 179460-AAV1 pAAV-EF1a-DIO-JEDI-2P-WPRE...

...tools that effectively transduce many mammalian cell types. AAV is used in a wide range of experiments, both...of the applicable transfer plasmid. We will help you gain legal usage of the transfer plasmid from the ...service lets scientists choose from over 4,000 transfer plasmids to be packaged in their choice of seven...chemogenetic, optogenetic, recombinase, and biosensor transfer plasmids in our repository are eligible for this...several important steps: MTA facilitation for the transfer plasmid, including legal approval and an implementation...

...including dual-transgenic recombinase-expressing mouse lines 2–9 and combinations of transgenic recombinase-expressing... Cre AND Flp, here expressed through a double transgenic animal strategy (PV-2a-Cre; SOM-IRES-Flp). Image...in a new window) of Flp-recombinase-expressing transgenic mouse and rat lines. The Fenno Lab maintains ... Rayport S. 2018. Dopamine neuron glutamate cotransmission evokes a delayed excitation in lateral dorsal...Neurons in Barrel Cortex Reveals Local and Long-Range Circuit Motifs. Cell Rep. 28(13):3450-3461. PubMed...Krook-Magnuson E. 2019. A novel population of long-range inhibitory neurons. Preprint (Link opens in a new...PubMed (Link opens in a new window) Mandelbaum G, Taranda J, Haynes TM, Hochbaum DR, Huang KW, Hyun M, Umadevi...

....AX with GRE or transgenic animal Oligodendrocytes - AAV-PHP.eB with GRE or transgenic animal Brain vascular....AX with GRE or transgenic animal L5 & Inhibitory - AAV-PHP.eB with GRE or transgenic animal L2/3 & L4...AAV.CAP-B10 with GRE or transgenic animal Excitatory - AAV.PHP.N with GRE or transgenic animal For PNS applications... pAAV-S5E2-GFP-fGFP E2 regulatory element GFP, membrane-targeted GFP Control Dimidschstein 192552 pAAV-CAG... viral prep format. Please note this does not guarantee viral vector packaging service, but lets us know...

... (~3 months), ease of care and breeding, and transparency of the embryo and larvae, it is easy to culture...standard toolbox of genetic methods, such as Tol2 transposons, Cre-mediated site-specific recombination, morpholinos...emerged as a model system for human disease and translational research, including the study of cancer, inflammation...-Cas9 system - Shuo Lin Lab Re-purposed Ac/Ds transgenic system enabling CRISPR/dCas9-interference (CRISPRi...zebrafish - Julian Lewis Lab PhOTO Zebrafish: A Transgenic Resource for In Vivo Lineage Tracing during Development...examples of popular tools to use in zebrafish, ranging from optogenetics to tools for studying diseases...vectors designed to streamline the production of transgenic zebrafish for studying neurodegenerative disorders...

...envelope protein, E; nucleocapsid protein, N; and membrane protein, M), including plasmids for protein production...Anderson et al., 2020). BSG plasmids - (CD147), transmembrane glycoprotein of the immunoglobulin superfamily...proteolysis of SARS-CoV Spike protein, activating its membrane fusion function (Bosch et al., 2008). TACE plasmids... protease (Mpro) activity reporters Reverse transcriptase, protease, and polymerase constructs for SARS-CoV...Llamas Required - Synthetic Nanobodies Against Membrane Proteins Return to top Pooled Libraries Pooled... SARS-CoV-2 infection to host cells . Signal transduction and targeted therapy, 5 (1), 283. https://doi.org...

...sequence and checks for off-target binding. Supports a range of Cas enzymes (Cas9 and Cas12) and organisms. Developed...gene inactivation, NHEJ, and HDR. Includes a wide range of reference genomes, including animals, plants,...and cytosine (CBE) base editors. Includes a wide range of reference genomes, including animals, plants,...Ui-Tei Lab . CRISPick (Link opens in a new window) Ranks and picks candidate CRISPRko/a/i sgRNA sequences...for off-target binding. Currently supports a wide range of organims. Developed by the Michael Boutros Lab...and Wente Zebrafish: gRNA cloning, in vitro transcription, injection gRNA core ; Cas9 ; optimized Cas9...105 KB Jaenisch CRISPR plasmid summary for transcriptional activation dCas9 activators sgRNA dual expression...

... the double-stranded siRNA and degrades one of the strands. The remaining single-stranded siRNA then binds...silencing is achieved through the delivery of a double-stranded RNA (dsRNA) that matches the mRNA target sequence...short interfering RNA). Delivery can occur via transfection or viral delivery of a plasmid. shRNAs are often...vector is taken up by a host, the viral genome is transcribed, producing the shRNA. Dicer, an RNase III enzyme...

...generate double strand breaks (DSBs). Nick Mutated Cas nickase enzymes generate single-strand DNA breaks. ...deletions. Insert CRISPR transposases insert large fragments of DNA. Transcriptional Regulation Activate dCas9...Prime Edit Cas nickase enzymes fused to reverse transcriptase install new genetic information into a specific..., or fused to a repressor peptide, inhibits transcription. Epigenetics dCas9 fused to an epigenetic modifier...

...reactivation, RNA editing, gene replacement therapy, RNA trans-splicing, and protein replacement to reverse the...cases Rett syndrome can be inherited. In females, random X inactivation results in approximately half of...disease severity. The MECP2 protein is a global transcriptional regulator of thousands of genes and studies...studies have suggested roles in transcriptional repression, activity dependent de-repression, chromatin remodeling...DNA and recruit known co-repressors or other transcriptional modifiers suggests the major function of MECP2...NTD) the M ethyl B inding D omain (MBD) a T ranscriptional R epressor D omain (TRD) the TRD contains a...Developing Human Brain - includes developmental transcriptome information, prenatal LMD microarray data, ISH...

...glycoprotein (G) gene, which is not required for the transcription or replication of the genome within infected...infected cells, but is required for transynnaptic spread (Wickersham et al., 2007a, Wickersham et al., 2007b...essential G protein is provided to the starter cell in trans , via delivery by an AAV. Since this starter cell... cell using the G-protein, which is supplied in trans . (3) Nascent G-protein coated RAVB is released ...Callaway EM. 2007b. Monosynaptic restriction of transsynaptic tracing from single, genetically targeted neurons... viral prep format. Please note this does not guarantee viral vector packaging service, but lets us know...

... 556:57–63 . https://doi.org/10.1038/nature26155 Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz...SpCas9 variants effectively double the targeting range of CRISPR/Cas9 within the human genome. Another ...known to produce off-target editing with SpCas9 (Amrani et al., 2018). When choosing a Cas9, remember to...formerly Cpf1), a nuclease that generates double strand breaks in target genes resulting in the formation...nature24049 Find plasmids from this paper at Addgene. Amrani N, Gao XD, Liu P, Edraki A, Mir A, Ibraheim R, ...018-1591-1 Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung J, Zhang F (2017) RNA editing...

...the most out of their meetings. Amanda Gunn ran randomized coffee trials for us at ASCB|EMBO 2019, matching...within a bin as Horizontal Transfer. This comes from horizontal gene transfer, which occurs when bacteria...in Atlanta, GA, in 2018. A lot of my experiences rang true with theirs.  It was at this dinner and the...Singletons logo. Image: James et al., 2020. Help me stranger - Finding mentors for the meeting Just like analysis...

...Genome engineering using the CRISPR-Cas9 system. Ran FA, et al. Nature Protocols Nov;8(11):2281-308; (2013)...dHJs are four-stranded branched structures that form when elongation of the invasive strand “captures” and...lesion results from breakage of both DNA strands - a double-strand break (DSB). DSBs can be caused by intracellular...proteins required for strand invasion and a primer for DNA repair synthesis. The ssDNA strand then displaces ...displaces one strand of the homologous DNA donor and can template repair off the other strand; this results in...pathways: classical double-strand break repair (DSBR), synthesis-dependent strand-annealing (SDSA), and break-induced...on the crossing strand (horizontally at the purple arrows) or on the non-crossing strand (vertically at...

...incorporated into the DNA by reverse transcription. The edited DNA strand displaces the unedited 5’ flap and...replace” gene editing method in which a reverse transcriptase (RT) is fused to the C terminus of Cas9 H840A...the target sequence where it nicks the non-target strand and generates a 3’ flap. The 3’ flap binds to the...Moloney murine leukemia virus (M-MLV) reverse transcriptase. Engineering of the PE enzyme and related components...including exploring new sources for the reverse transcriptase. Many PE enzymes and related tools are available...

... Material Transfer Agreements Most Addgene orders must be accompanied by a material transfer agreement...industry scientists. Please note this does not guarantee availability but lets us know what plasmids would...agreement (MTA), including transfers to for-profit entities. The MTA is a legal agreement between the recipient... the depositing institution(s). It governs the transfer of biological materials and intellectual property...Please see the Sample Agreements on our Technology Transfer Information page for more information. To learn...

...associated with the disease and implicate a wide range of cellular processes. The vast majority of cases...deposition with APOE2 constructs . (Hudry et al. Sci Transl Med. 2013) Express human DNMT1 with a Myc tag. ...vesicular brain cells and more. Measure synaptic transmission with improved glutamate indicators . (Aggarwal...Differentiation Factors Find plasmids used to transform iPSCs and fibroblasts into neurons and more. Convert...plasmids expressing SOX10, OLIG2, and NKX6.2 transcription factors for rapid and efficient generation of...astrocytes to dopaminergic neurons using three transcription factors in a single polycistronic lentiviral...

...optimization of an orthogonal translation system with enhanced biological tolerance . Molecular Systems Biology...phosphorylation is one of the most abundant forms of posttranslational modification in cells and research into its...Lajoie et al., 2013) and improving the orthogonal translation system to make phosphorylated proteins with one... , and an optimized phosphoserine orthogonal translation system, pSerOTS-C1* (V70) (Plasmid #188537) .... with the optimized phosphoserine orthogonal translation system, SepOTSλ (Plasmid #68292) , they were ...

...easily and inexpensively generate R-mAbs by transiently transfecting the plasmids into mammalian tissue culture...leader sequence (L) for translocation of the heavy chain across the ER membrane. PCR-mediated fusion of...leader sequence (L) for translocation of the light chain across the ER membrane. Downstream of the insert... Mouse IgG2a 206524 Anti-SNAT1 transporter [N104/37R] SNAT1 transporter Rat Mouse IgG2a 206525 Anti-Ankyrin-G...high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells...generation R-mAbs plasmids, production of R-mAbs in transfected cells and their validation can be found below...heavy (blue) chains as driven by two CMV promoters (orange). Hybridoma-derived V L and V H domain PCR products...

...tools that effectively transduce many mammalian cell types. AAV is used in a wide range of experiments, both... Packaged on Request service. You can choose a transfer plasmid from our repository and request packaging...Monster, Brainbow Molecular Tools Tetracycline Transactivators, Affinity Purification, Cell Ablation, CRISPR...Testing Panel for the Eye and Brain For enhanced transduction of the eye and other tissues All Serotypes If...