We narrowed to 210 results for: cycs

1. Rabies and Neuronal Tracing

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   Blog Post

   ...Once this first order infection occurs, subsequent cycles of viral replication and retrograde transmission...

2. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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   Blog Post

   ...nourseothricin (clonNat), spectinomycin, streptomycin, tetracycline, combinations of the above, or other. High...

3. 6 Tips for Grant Writing

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   Blog Post

   ... to improve your application for the next grant cycle.  Many thanks to our Guest Blogger, Sean Mac Fhearraigh...

4. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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   Blog Post

   ...Using the Tet-on system, his lab has created a tetracycline cas9 inducible knock-out system. His lab is ...

5. Academic vs. Industry Postdocs

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   Blog Post

   ...industry postdoc will free you of the never-ending cycle of grant-writing that is academic life. Of course...

6. Hot Plasmids and Viral Preps - May 2021

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   Blog Post

   ...where gRNA expression is induced by anhydrous tetracycline. Three new anti-CRISPRs were identified that...

7. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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   Blog Post

   ...stop emerging at night. An incubator with a light cycle makes for very predictable work! When flies first...

8. Course-Based Undergraduate Research Experiences (CUREs)

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   Blog Post

   ...work on malate dehydrogenase (MDH), a citric acid cycle enzyme he studied during graduate school. As a stable...

9. Simplify Cloning with in vivo Assembly

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   Blog Post

   ... different templates. All PCRs are run as an 18 cycle, 25 μl single-tube reaction. If you are synthesizing...

10. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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    Blog Post

    ... tag (Figure 1c). Click chemistry (e.g., transcyclooctene–tetrazine)– Nonnatural amino acids can be ...

11. Plasmids 101: Colony PCR

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    Blog Post

    ...dNTPs and then incubate with a standard PCR thermocycling program. One key difference is the plasmid DNA...

12. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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    Blog Post

    ...researchers to study aspects of the EBOV replication cycle under BSL-2 conditions (4). What is a minigenome...

13. Ras Pathway

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    Collection

    ...exchange factor 2 CCND CCND1 CCND2 CCND3 Cyclin D CDK CDK4 CDK6 Cyclin-dependent kinase CNKSR CNKSR1 CNKSR2...

14. Hot Plasmids: Fall 2024

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    Blog Post

    ...with Cas9 on an integrative plasmid containing tetracycline repressor TetR and resistance selection marker...

15. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

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    Blog Post

    ...hydra have asexual and sexual reproductive life cycles. One can inject plasmid DNA in the eggs to get ...

16. Bioinformatics at Addgene

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    Blog Post

    ...Now, we could iterate our development and testing cycle faster due to the ease of jumping back and forth...

17. CRISPR-based Genome Editing Systems For Editing in E. coli

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    Blog Post

    ... plasmids for multicopy integration. (B) Anhydrotetracycline (aTc) induces expression transposases leading...

18. Fluorescent Proteins 101: Aptamer Fluorophores

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    Blog Post

    ...while aptamers are more stable and can go through cycles of denaturation/renaturation. In vivo assays ...

19. How to Write a Scientific Review Article

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    Blog Post

    ...is probably “a normal day.” The way I broke this cycle was to just start writing. A word of advice: when...

20. Plasmids 101: Gateway Cloning

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    Blog Post

    ...vector like pLenti CMV Puro DEST (w118-1) or the doxycycline-inducible pLIX_403. The chosen attR destination...