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...Promega.com. ID Plasmid Description Industry Plasmid Technology Overview This collection includes specialized Promega...contains plasmids developed by Promega scientists and designed to streamline molecular and cellular biology experimental...support a range of applications. The technology includes NanoLuc, HaloTag, SmBiT, LgBiT, HiBiT, and more... a new window) is an energy transfer technique designed to measure molecular proximity in living cells...

...components of the array recognize individual nucleotides. For over ten years, zinc finger (ZF) arrays ...that came out in the spring of 2011, the groups describe a set of customized plasmids that can be used ... a TALEN kit through Addgene. This kit, first described in Nature Biotechnology in August, uses a serial...in Nature Biotechnology early in 2011 and they described their kit for assembling TAL effector arrays in...effector and TALEN kits here . References Efficient design and assembly of custom TALEN and other TAL effector-based...

... Guide to Plasmid Pooled Libraries . ID Name Description Genes/Insert Selection PI Human knockout pooled...2A-Blast-WPRE) Zhang Pooled Barcoding Libraries ID Name Description PI 115643 Morris CellTag Barcode Library Version...Version 1 of the Celltag library contains 19973 barcodes to combinatorially index cells for single-cell...Version 2 of the Celltag library contains 4934 barcodes to combinatorially index cells for single-cell...Version 3 of the Celltag library contains 5737 barcodes to combinatorially index cells for single-cell...

...CRISPR plasmids that have been designed for use in yeast....Resources The following CRISPR plasmids have been designed for use in yeast. To find emtpy gRNA plasmids,...PI Publication Return to top Nick ID Plasmid Description Gene/Insert Promoter Selectable Marker PI Publication... CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...

...CRISPR plasmids based on Cas13 designed to precisely edit RNA targets....mutation reduces off-target effects of ADAR2 by destabilizing its RNA binding. Editors carrying the delta-...or search the tables below for CRISPR plasmids designed for RNA editing. To learn more about RNA editing... CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...

...CRISPR guides. The first one binds to sfgfp with either 0, 10, 11, 14, or 20 matching nucleotides, the ...also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The David Bikard Lab at...mcherry with either 0, 11, 18, or 20 matching nucleotides. Together, these plasmids allow to explore the...

... chemogenetic receptors: Designer Receptors Exclusively Activated by Designer Drugs (DREADDs) and Pharmacologically...opto-chemogenetic actuator. For more detailed descriptions of chemogenetic receptors, ligands, and how ...preparations of many chemogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter Eligible for...

...recombination with the shuttle plasmid. This collection provides a curated selection of popular plasmids that can...for shuttle plasmids with inserts. ID Plasmid Description PI 16402 pShuttle For production of viruses containing...adenoviral vector production. ID Plasmid / Kit Description PI 16400 pAdEasy-1 Adenoviral vector for use ... for adenoviral vector production. ID Strain Description PI 16398 BJ5183 E. coli strain for recombination...

...138152 pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro Lentiviral vector expressing destabilized EGFP...CoV-2), modification, or variant. ID Plasmid Description Mutations Industry PI Due to export control regulations...neutralizing activity are listed below: ID Plasmid Description PI 44965 NL4-3 mCherry Luciferase Lentiviral ...

...after the promoter, and so on. The following table provides information for the assembly standards most commonly...will need to be reverse complimented for primer design. The restriction enzymes used for assembly are ..., NotI , PstI Scar: GCTAGT Features: 6 bp scar encodes Ala-Ser; Allows for in-frame fusions; NheI is rare...Enzymes: BamHI , Xhol Scar: GGATCT Features: Scar encodes Gly-Ser in-frame with prefix start codon; Employs...

... cleavage sites. Other protein tags and signal peptides are used to enhance protein solubility and to ...Commonly used tags, cleavage sites, and signal peptides include: Epitope tags: 6xHis, Flag, Strep II, ...protease, factor Xa, enterokinase, thrombin Signal peptides for periplasmic localization: PelB, MalE, OmpA...Anhydrotetracycline (aTc) Escherichia coli Stanley Qi 11518 pDest-527 T7-lacO Lactose/IPTG Escherichia coli Dominic...a collection of CRISPR plasmids that have been designed for their use in bacteria in our Bacterial CRISPR...

...contain a viral genome encoded on RNA. The genome includes a reverse transcriptase and an integrase to insert...plasmids that have inserts. ID Plasmid 5'LTR Description PI 1780 pBABE-neo largeTcDNA MoMLV Expression...Gamma-Retroviral Packaging Plasmids ID Plasmid Description PI 8449 pUMVC Contains MoMLV gag and pol . Use...Gamma-Retroviral Envelope Plasmids ID Plasmid Description PI 1733 pCI-VSVG VSV-G recommended for the FELIX...

...required for AAV viral replication. This collection provides a curated selection of transfer, packaging, and... packaging eligibility, and more. ID Plasmid Description PI Return to top AAV Packaging Plasmids Packaging...for all available serotypes. ID Plasmid Capsid Description PI 112862 pAAV2/1 AAV1 AAV packaging plasmid,...repository for AAV vector production. ID Plasmid Description PI Return to top Other Resources Addgene's Viral...

...using in mouse transgenics. Designing an Easi-CRISPR knock-in allele To design a knock-in allele at a given... PAM or guide mutation. Designing an Easi-CRISPR conditional allele The design for a floxed conditional...successfully used guides targeting the same or complementary strands, as well as guides facing towards or...How to design a conditional allele with Easi-CRISPR. As in the knock-in example, you’ll design your ...with your desired insertion feature (e.g., a fluorescent protein), you can amplify the desired region using... Figure 5: How to design Easi-CRISPR repair templates using Addgene plasmids. Design PCR primers to add...arduous. First, you’d construct a plasmid with your desired changes, long homology arms and a selection marker...

...relaxed gRNA design parameters. To demonstrate the effectiveness of their library design, Moffat laboratory...-strand DNA (ssDNA) oligonucleotides (oligos), and thereby rapidly create desired allele combinations ... pCAG-T7-TALEN(Sangamo)-Destination series, pCAG-Golden-Gate-Esp3I-Destination Takashi Yamamoto pcDNA-TAL-NC2...vector, and besides being used for restoring strain prototrophy, can also aid in designing self-establishing...assay. In their recent publication, Kroeze et al. describe their enhanced TANGO arrestin recruitment assay...optimized, expression validated GPCR sequences to any desired backbone.  Kroeze, et al. Nat Struct Mol Biol....plasmids with the Cross-disciplinary Integration of Design Automation Research lab Moduclar Cloning (CIDAR...

...clone in their desired DNA sequences. Gateway technology relies on the two reactions described below: The ...pLenti CMV Puro DEST (w118-1) or the doxycycline-inducible pLIX_403. The chosen attR destination vector will...between the entry clone and destination vector creates two products: the desires expression clone and a byproduct...produce the desired expression clone. This is possible thanks to the Gateway vectors’ design. They have...vector systems. With the appropriate entry and destination vectors, one can use Gateway to clone a gene ...generated entry clone and the attR sites of the destination vector. This reaction is catalyzed by the LR ...containing the ccdB gene is excised from the destination vector.  Once the BP and/or LR reactions are ...

....  These primers are typically ~70 nucleotides long (20 nucleotides that anneal to the DNA sequence of..., the substrate should be ~70-100 nucleotides long with the desired alteration(s) located in the center...genomes. 2) Designing ssDNA oligos that avoid MMR activation:  There are two ways to design oligos that...tube. The donor DNA substrate only requires ~50 nucleotides of homology to the target site for recombination... be explained in greater detail. Substrate DNA design and generation Expression of lambda red recombination...antibiotic resistance cassette.   Substrate DNA Design and Generation Whether you use a linear dsDNA or...insertions or deletions greater than approximately 20 nucleotides, while ssDNA substrate is best for point mutations...

...preprint title or a general description of the deposit. Select your desired distribution status: Hold...choose from one of the following descriptions for your plasmid: Encodes insert(s) A plasmid with one or...should describe how your plasmid is intended to be used. For instance, if your plasmid is designed to allow...Unpublished or pre-publication plasmids" and set a desired distribution status. You can submit "Published ...plasmids with the original publication where you described them. Search for your article as you would search...distribute, or Addgene finds your journal publication describing the plasmids, or (for deposits submitted after...selected your publication or entered your deposit description, the next page will allow you to add plasmids...

...template vector design guidelines? A7: A few notes below are considerations for designing HR donor. Generally...usually pick 3–6 guides around the region to find the most efficient guide (most guides would work, but... this ‘split RNA’ design is not as efficient in genome editing as the second design (in pX330/PX335), ... CRISPR plasmid tools and numerous experimental design decisions to make, it makes sense that scientists...CRISPR forum's most popular questions and answers. Designing your CRISPR genome editing experiment Q1: Should...comparable efficiency to the optimized chimeric design but with better accuracy (in other words, lower...nickase system is based on the Cas9 D10A nickase described in Figure 4 of the Cong et al. (2013) Science ...

...list of Fragmid destination vectors currently available. Table 1: Fragmid destination vectors Organism...website, which breaks the design process down into four easy steps, provides the real value for the user...potential of them not working, or spend a few weeks designing and cloning the vectors you actually want. But... Fragmid is a modular vector assembly process designed for a quick, plug-and-play approach to cloning...et al., 2024). It reduces the amount of time to design and create a new vector to four days for an experienced...high school interns, were able to use Fragmid to design and clone their vectors in five days. Did you say...s modular assembly structure takes a lot of the design work out of the plasmid creation process. Each ...