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We narrowed to 610 results for: gan

Showing: 121 - 140 of 610 results

  1. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    ...designer ligands that can be simply introduced by injection. The main challenge in using ligands is ensuring...at broad tissue or system-wide targeting, as the ligand can reach target cells throughout the body. Controlling...chemogenetics, while different concentrations of ligands can be injected, this is not as precise and the...brain. One way to address this is by working with organisms with light accessible brains, such as zebrafish...while also being selective and effective. A new ligand from the Bryan Roth lab and Takafumi Minamimoto...

  2. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...affinity for the ligand, especially if the modified amines are located near the ligand binding site. A ...unbound material to calculate the amount of bound ligand (after subtracting out non-specific binding). Historically...dimers, may influence binding kinetics of the target ligand. Another important consideration is whether the...the affinity of an experimental protein-ligand interaction approaches that of GST-glutathione. Biotin-...measurement of high affinity (i.e. sub-nanomolar) protein-ligand interactions. Avidin and its derivatives have four...

  3. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...many organisms. When supplemented with adenine analogs such as 2-fluoroadenine (2-FA), organisms with ...Zurich. He is fascinated by how organisms exquisitely and robustly self-organize at the cellular and multicellular...Fortunately, researchers studying plants and other organisms lacking this innate immunity need not worry and...plants Once you’ve introduced your RNAi into your organism, how do you identify the actively silenced ones... of APT and any gene target of interest, only organisms that are actively silencing to a sufficient degree...

  4. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...serve as slaves.  Meganucleases In 1989 the first ever DNA-editing enzymes, meganucleases, were discovered...capability of transforming the genetic code of living organisms and its societal implications even before the ...pneumoniae bacteria could be transformed from one organism to the other. This is now famed as the Griffith...genetics and SciFi.  Scientists around the world began looking for the magic molecule that serves as the...in 1990, just a year after the discovery of meganucleases. The storyline involves the resurrection of ...of all the endonuclease-based gene editors (meganucleases, ZFNs, TALENs, and CRISPR-Cas) is that they ...

  5. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ... post was originally written in 2014 by Kendall Morgan and updated in 2022 by Lucie Wilson. Lucie is an...used to generate mutations in a wide range of organisms, but its potential to efficiently correct disease...functional correction of the gene in clonally expanded organoids. “CRISPR in adult stem cells has huge potential...potential, because the genomes of adult stem cells in organoid cultures are stable,” said Gerald Schwank, first..., however, because the disease affects multiple organ systems. Nevertheless, the findings are proof-of-principle...of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients." Cell Stem Cell. ...

  6. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...easily deliver their “cargo” to target cells and organisms, and when that cargo is designed to make you sick...biological tools In nature, viruses infect cells and organisms (which we refer to as hosts) via viral particles...and fuse with. In nature, this determines which organisms and cells they infect. In the lab, this means ...can also be directly injected into hard-to-reach organs, such as the brain. Secondly, some viruses have... makes them ideal for applications with whole organisms. Finally, viral delivery is typically straightforward...strong immune response when introduced into an organism. This feature makes them an attractive tool for...

  7. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR...

  8. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ... C. elegans, but activation of Raf-MEK-ERK signaling in the hypodermis and gut cells of C. elegans causes... a signaling pathway in the context of a whole organism, but also allows for precise activation of a kinases...utility of  photoswitchable kinases in vivo with C. elegans. Researchers commonly use rectal bacterial infection...Zhou et al also used a photoswitchable CDK5 in C. elegans. See figures 4B and C in Zhou et al for details..., but they need to be expressed in a tissue or organism that blue and violet light can penetrate. How ...

  9. Hot Plasmids - October 2020

    Type
    Blog Post
    ...synaptic proteins Fluorescent protein system for C. elegans Fluorescent timers for studying cell cycle length...fluorescent proteins system for Caenorhabditis elegans By Jennifer Tsang Fluorescent proteins (FPs) traditionally...traditionally introduced in Caenorhabditis elegans on extrachromosomal arrays can lead to artifacts due...locus, but introducing large genetic changes in C. elegans with CRISPR can be difficult since it requires ...use CRISPR to introduce large FP genes into C. elegans? By using a split FP approach. Split-wrmScarlet...they fuse and fluoresce. The team engineered C. elegans cell lines to express wrmScarlet1-10 unattached...

  10. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...result of the content. Here are some of my tips to organizing and running a memorable panel discussion. Check...make it look effortless to the audience, but the organizers and moderator should have a well-planned approach...panelists to the moderator. The moderator and organizers should develop a list of 4-6 pre-event interview...interview questions. The moderator (possibly with an organizer) should set up a 30 minute call with each panelist...example, at a recent event on nonprofit science organizations, I asked audience members to stand and describe...networking time. Both the moderator and program organizers can help make sure the event stays on schedule...

  12. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...been an invaluable resource for studying the organization and function of cellular systems. FPs have been...engineering FP fusions associated with cellular organelles, scientists have been able to study many cellular... by evaluating visual phenotypes. Improving C. elegans fluorescent protein tagging with SapTrap The Jorgensen...SapTrap to improve CRISPR genomic tagging in C. elegans. SapTrap offers scientists a convenient one tube...tagging systems For those who do not work with C. elegans, several groups have designed modular toolkits ...CRISPR/Cas9 Gene Modification in Caenorhabditis elegans.” Genetics. 202(4) (2016):1277-88. PubMed PMID:...

  13. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...protein and (2) a synthetic fluorophore-contianing “ligand” that binds to the tag. Like most good ideas in...selective interaction between a bisarsenical dye ligand (e.g., FlAsH, ReAsH) and a short genetically encoded...specifically and irreversibly with a small substrate ligand motif attached to a fluorophore (Figure 1b). ...catalyze the covalent attachment of a fluorophore ligand to a peptide tag (Figure 1c). Click chemistry...labeling strategies is fluorogenicity, meaning the ligand exhibits low fluorescence when free in solution...the availability of fluorescent and fluorogenic ligands. New fluorophores As a corollary to these innovative...

  14. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    ...annotating, and storing life science protocols. Since we began sharing this protocol on the Addgene website in ...About Developing Lab Management Software    Organizing and sharing your updated protocols So that’s ... mobile) tools are available for scientists to organize and share their scientific protocols. Here are...OpenWetWare (see below), and now Addgene. I just began uploading our cloning protocols to the site and ...streamline your lab management and you want the works: organizing reagent storage and inventory, managing orders... can offer a one-stop solution to your lab's organization and management needs. With Quartzy you can share...

  15. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...Using a technique called systematic evolution of ligands by exponential enrichment (SELEX), some 1010 oligonucleotides...of the next two decades, these nucleotide-based ligand binders would prove to be highly adaptable tools...structure (see Fig. 1a). When the aptamer binds its ligand, the stem loop is disrupted, removing the quencher... dimerization for activation, only in this case ligand binding would bring the relevant ends together ...aptamers fluoresce until they bind to the appropriate ligand, but relying on a lack of fluorescence for readout...response. Aptamers can be selected for these small ligands. References 1. Ma, Hanhui, et al. “Multicolor...

  16. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...but, ummmm, what is it? Reddit is a volunteer-organized platform where users can participate in forum ...trolls. 3. The AMA process was simple and well organized. You can check out the r/Science AMA Submission...only small downside to the AMA from my end as an organizer/promoter was that I didn’t have the direct link... science talk here). Finally, my main content organizer is the amazing app Evernote. I have a paid account...which gives me all the features I need to save, organize and easily find the resources that I come across...do without it. So get yourself a good content organizer and build your own professional development etc...

  17. Synthetic Biology - Overview

    Type
    Collection
    ...plasmid-strain functional pairs. Browse Plasmids by Model Organism Bacterial Plant Yeast Mammalian Worm Fungal Algal...

  18. Adenovirus Plasmids

    Type
    Collection
    ...adenovirus plasmids in either mammalian cells or microorganisms, including bacteria and yeast. Two plasmids...

  19. Introducing Addgene's Microbiology Pages

    Type
    Blog Post
    ...Collection are sorted by organism (see screenshot above). Don’t see your favorite organism listed? Help us serve...serve you! Let us know about other organisms we should include by emailing us at [email protected]. If...to narrow your keyword search to one of these organisms. Help us continue to develop resources for your...like to get the word out about a particular microorganism, microbial technique, or microbial technology...

  20. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...critical in the business world. Companies and organizations that avoid all conflict can fall into “groupthink...individuals involved, and also for the labs, organizations, or businesses as a whole. When I’m attempting... sharing gets more challenging the bigger an organization gets, and creating new avenues of communication...should be communicated is necessary in a large organization, because confirming that every person has read...attempting to resolve conflicts within a given organization, it can help tremendously to have as much context... solutions that benefit themselves and their organization. Resolving conflicts is great, but avoiding ...
Showing: 121 - 140 of 610 results