...transducer complexes that are activated in response to a ligand binding a GPCR of interest. How does TRUPATH work...intracellular surface of the GPCR. Binding of a ligand to the extracellular surface of the GPCR causes...coelenterazine, followed by the addition of the endogenous ligand or drug. In the inactive state, the luminescent...emission. Following activation in response to the ligand, the complex will disassociate, resulting in a ...across the transducerome for a specific receptor-ligand pair. They further used TRUPATH to analyze a panel...This collection can be used to study endogenous ligands, the pathways they activate, as well as the activation...

...tethered ligands hold the ligand in close proximity to the receptor, when exposed to light, the ligand engages...photoswitchable tethered ligands (PTL), and photoswitchable, orthogonal, remotely-tethered ligands (PORTLs). OptoXRs... them. PORTLs use a similar concept, except the ligand is attached to a protein binding partner or nanobody..., McKnight, D. J., Yoshizawa, S., Kato, H. E., Ganguli, S., & Deisseroth, K. (2019). Cortical layer–specific...

...affinity for the ligand, especially if the modified amines are located near the ligand binding site. A ...unbound material to calculate the amount of bound ligand (after subtracting out non-specific binding). Historically...dimers, may influence binding kinetics of the target ligand. Another important consideration is whether the...the affinity of an experimental protein-ligand interaction approaches that of GST-glutathione. Biotin-...measurement of high affinity (i.e. sub-nanomolar) protein-ligand interactions. Avidin and its derivatives have four...

...Collections A modular set of plasmids from a depositor organized by a common use. Click here to view all collections...

...Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR...

...Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR...

...yielded TALENs which function efficiently in: C. elegans ( Wood et al., Science 2011 ) Zebrafish ( Sander...

...many organisms. When supplemented with adenine analogs such as 2-fluoroadenine (2-FA), organisms with ...Zurich. He is fascinated by how organisms exquisitely and robustly self-organize at the cellular and multicellular...Fortunately, researchers studying plants and other organisms lacking this innate immunity need not worry and...plants Once you’ve introduced your RNAi into your organism, how do you identify the actively silenced ones... of APT and any gene target of interest, only organisms that are actively silencing to a sufficient degree...

...serve as slaves.  Meganucleases In 1989 the first ever DNA-editing enzymes, meganucleases, were discovered...capability of transforming the genetic code of living organisms and its societal implications even before the ...pneumoniae bacteria could be transformed from one organism to the other. This is now famed as the Griffith...genetics and SciFi.  Scientists around the world began looking for the magic molecule that serves as the...in 1990, just a year after the discovery of meganucleases. The storyline involves the resurrection of ...of all the endonuclease-based gene editors (meganucleases, ZFNs, TALENs, and CRISPR-Cas) is that they ...

... post was originally written in 2014 by Kendall Morgan and updated in 2022 by Lucie Wilson. Lucie is an...used to generate mutations in a wide range of organisms, but its potential to efficiently correct disease...functional correction of the gene in clonally expanded organoids. “CRISPR in adult stem cells has huge potential...potential, because the genomes of adult stem cells in organoid cultures are stable,” said Gerald Schwank, first..., however, because the disease affects multiple organ systems. Nevertheless, the findings are proof-of-principle...of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients." Cell Stem Cell. ...

...easily deliver their “cargo” to target cells and organisms, and when that cargo is designed to make you sick...biological tools In nature, viruses infect cells and organisms (which we refer to as hosts) via viral particles...and fuse with. In nature, this determines which organisms and cells they infect. In the lab, this means ...can also be directly injected into hard-to-reach organs, such as the brain. Secondly, some viruses have... makes them ideal for applications with whole organisms. Finally, viral delivery is typically straightforward...strong immune response when introduced into an organism. This feature makes them an attractive tool for...

... C. elegans, but activation of Raf-MEK-ERK signaling in the hypodermis and gut cells of C. elegans causes... a signaling pathway in the context of a whole organism, but also allows for precise activation of a kinases...utility of  photoswitchable kinases in vivo with C. elegans. Researchers commonly use rectal bacterial infection...Zhou et al also used a photoswitchable CDK5 in C. elegans. See figures 4B and C in Zhou et al for details..., but they need to be expressed in a tissue or organism that blue and violet light can penetrate. How ...

...synaptic proteins Fluorescent protein system for C. elegans Fluorescent timers for studying cell cycle length...fluorescent proteins system for Caenorhabditis elegans By Jennifer Tsang Fluorescent proteins (FPs) traditionally...traditionally introduced in Caenorhabditis elegans on extrachromosomal arrays can lead to artifacts due...locus, but introducing large genetic changes in C. elegans with CRISPR can be difficult since it requires ...use CRISPR to introduce large FP genes into C. elegans? By using a split FP approach. Split-wrmScarlet...they fuse and fluoresce. The team engineered C. elegans cell lines to express wrmScarlet1-10 unattached...

...result of the content. Here are some of my tips to organizing and running a memorable panel discussion. Check...make it look effortless to the audience, but the organizers and moderator should have a well-planned approach...panelists to the moderator. The moderator and organizers should develop a list of 4-6 pre-event interview...interview questions. The moderator (possibly with an organizer) should set up a 30 minute call with each panelist...example, at a recent event on nonprofit science organizations, I asked audience members to stand and describe...networking time. Both the moderator and program organizers can help make sure the event stays on schedule...

...Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR...

...been an invaluable resource for studying the organization and function of cellular systems. FPs have been...engineering FP fusions associated with cellular organelles, scientists have been able to study many cellular... by evaluating visual phenotypes. Improving C. elegans fluorescent protein tagging with SapTrap The Jorgensen...SapTrap to improve CRISPR genomic tagging in C. elegans. SapTrap offers scientists a convenient one tube...tagging systems For those who do not work with C. elegans, several groups have designed modular toolkits ...CRISPR/Cas9 Gene Modification in Caenorhabditis elegans.” Genetics. 202(4) (2016):1277-88. PubMed PMID:...

...bar or sort buttons to find plasmids based on: Organism or species (e.g., human, watermelon, Streptomyces...

...detection of low affinity extracellular receptor-ligand interactions. Kerr SJ and Wright GJ. J. Vis. Exp...

...protein and (2) a synthetic fluorophore-contianing “ligand” that binds to the tag. Like most good ideas in...selective interaction between a bisarsenical dye ligand (e.g., FlAsH, ReAsH) and a short genetically encoded...specifically and irreversibly with a small substrate ligand motif attached to a fluorophore (Figure 1b). ...catalyze the covalent attachment of a fluorophore ligand to a peptide tag (Figure 1c). Click chemistry...labeling strategies is fluorogenicity, meaning the ligand exhibits low fluorescence when free in solution...the availability of fluorescent and fluorogenic ligands. New fluorophores As a corollary to these innovative...

...annotating, and storing life science protocols. Since we began sharing this protocol on the Addgene website in ...About Developing Lab Management Software    Organizing and sharing your updated protocols So that’s ... mobile) tools are available for scientists to organize and share their scientific protocols. Here are...OpenWetWare (see below), and now Addgene. I just began uploading our cloning protocols to the site and ...streamline your lab management and you want the works: organizing reagent storage and inventory, managing orders... can offer a one-stop solution to your lab's organization and management needs. With Quartzy you can share...