To Codon Optimize or Not: That is the Question
Type
Blog Post
...different organisms. There are several codons that encode the same amino acid. Each organism, however,...the genetic code is universal, many different organisms actually prefer certain codons over others for... biologist who wants to express genes from one organism in another? Let’s take a look at codon usage and...optimize codons for expression in a particular organism.
Why is codon usage important?
Translation and...for codon-usage of 257,468 genes across 8,792 organisms (Nakamura et al., 2000).
What is codon optimization...expression by changing synonymous codons based on an organism's codon bias. The idea is that scientists can make...mutations throughout a gene of interest based on an organisms codon usage bias to increase translational efficiency...