We narrowed to 147 results for: pam
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TypeBlog Post...antibiotic treatment. To facilitate these studies, Pamela Silver’s lab developed a synthetic mark and recapture...
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Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria
TypeBlog Post...Central PMCID: PMC5180612. Libby, Elizabeth A., and Pamela A. Silver. "Harnessing undomesticated life." Nature... -
Negative Can Be Positive: Open AAV Data with Addgene
TypeBlog Post...High-magnification images immuno-stained against dopamine beta-hydroxylase (DBH, magenta) to label norepinephrine... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog Post...tools, techniques, and optogenetic application to dopamine-mediated reinforcement." Neuron 72.5 (2011): 721... -
Hot Biosensors 2022: Year-End Roundup
TypeBlog Post... this year. We’ve added new viral preps of the dopamine sensor dLight1.2 and voltage sensor Ace2N and ... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog Post...Liu, and S.C. Zhang, Directed differentiation of dopamine neurons from human pluripotent stem cells. Methods... -
Using AAV for Neuronal Tracing
TypeBlog Post...in only specific cell types, for example only dopaminergic neurons, neuronal tracing has the added benefit... -
Neurodegeneration Plasmid Collection
TypeCollection... Spinocerebellar Ataxia Pietro De Camilli 194242 pAM/SAR-CAG-aSyn(A53T)/HA-WPRE-bGHpA SNCA HA SAR-CAG ...Parkinson's Michael J Fox Foundation MJFF 194247 pAM/CBA-eGFP-miRaSyn(human)x3-WPRE-bGHpA SNCA GFP CAG...Parkinson's Michael J Fox Foundation MJFF 194248 pAM/CBA-eGFP-miRaSyn(mouse)x3-WPRE-bGHpA SNCA GFP CAG...T7 Huntington's Pamela Bjorkman 11514 pET32a-HD39Q HTT His, Trx T7 Huntington's Pamela Bjorkman 11515 ...11487 pET32a-HD16Q HTT His, Trx T7 Huntington's Pamela Bjorkman 11503 pBabe neo human Frataxin FXN Friedreich...11515 pET32a-HD46Q HTT His, Trx T7 Huntington's Pamela Bjorkman 12165 pSico Dnmt1 DNMT1 GFP CMV Hereditary...NR4A2 NE CMV Parkinson's Shu Leong Ho 102364 pSIN-PAmCherry-mSNCA-NE SNCA NE EF-1 alpha Parkinson's Shu Leong... -
Fluorescent Protein Guide: Empty Backbones
TypeCollection...Expression PAmCherry1 564 595 8 6.3 23 min Monomer pPAmCherry1-N1 - Mammalian Expression pPAmCherry1-C1 - Mammalian...pBAD/HisB-PAmCherry1 - Bacterial Expression PAmCherry2 570 596 13 6.2 18 min Monomer pPAmCherry2-C1 - Mammalian...Expression PAmCherry3 570 596 5 6.2 25 min Monomer pPAmCherry3-C1 - Mammalian Expression PAmKate 568 628 ...628 5 5.6 19 min Monomer pPAmKate-N1 - Mammalian Expression pBAD/HisB-PAmKate - Bacterial Expression PAiRFP1... -
Fluorescent Protein Guide: Biosensors
TypeCollection...monitoring dopaminergic activity in vivo. Nat Methods. 2023 Nov 30. Yulong Li Dopamine Dopamine sensors ...2020.02.07.939504 Loren Looger , J. Julius Zhu Dopamine Red and green GRAB_DA3 dopamine sensors Improved green and red...monitoring dopaminergic activity in vivo. Nat Methods. 2020 Nov;17(11):1156-1166. Yulong Li Dopamine Ultrafast...neuronal imaging of dopamine dynamics (dLight1.1-1.5) Ultrafast neuronal imaging of dopamine dynamics with ...science.aat4422. Lin Tian Dopamine Yellow or Red genetically encoded dopamine sensor YdLight1 and RdLight1...RdLight1 An expanded palette of dopamine sensors for multiplex imaging in vivo. Nat Methods. 2020 Nov;17(... -
Brain Initiative Collection
TypeCollection...pAAV-hSyn-GRAB_DA1m express the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1m in neurons Yulong Li 113050...pAAV-hSyn-GRAB_DA1h express the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1h in neurons Yulong Li 113050...pAAV-hSyn-GRAB_DA1h express the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1h in neurons Yulong Li 115892...pAAV-CAG-dLight1.3b For expression of the genetically encoded dopamine sensor dLight1.3b Lin Tian 125560-AAV5 pAAV-CAG-dLight1.3b...pAAV-CAG-dLight1.3b For expression of the genetically encoded dopamine sensor dLight1.3b Lin Tian 125560-AAV9 pAAV-CAG-dLight1.3b...pAAV-CAG-dLight1.3b For expression of the genetically encoded dopamine sensor dLight1.3b Lin Tian 125707-AAV9 pAAV-CAG-RdLight1...pAAV-CAG-RdLight1 Expresses the red genetically encoded dopamine sensor RdLight1 in mammalian cells. Lin Tian 125708... -
mTOR Pathway
TypeCollection...Resources The m echanistic or m ammalian t arget o f r apamycin (mTOR) is a key metabolic regulator controlling...threonine kinase 11 mTOR Mechanistic target of rapamycin NF1 Neurofibromin 1 PRAS40 Also known as AKT1S1...associated protein 1 mTOR Mechanistic target of rapamycin p53 TP53; tumor protein p53 PDK1 Pyruvate dehydrogenase...10.1016/j.cell.2012.03.017. PubMed PMID: 22500797 . Rapamycin: one drug, many effects. Li J, Kim SG, Blenis ... -
Fluorescent Protein Guide: Subcellular Localization
TypeCollection...31930 pPAmCherry-a-tubulin Microtubules alpha-tubulin PAmCherry Vladislav Verkhusha 31949 pPAmCherry-b-actin...Nucleus NLS YFP Connie Cepko 158000 pCaggs-NLS-PAmCherry1-GSS-EGFP Nucleus NLS mGold Francois St-Pierre...-actin Actin Filaments beta-actin PAmCherry Vladislav Verkhusha 79990 pKeratin-miRFP703 Intermediate Filaments... -
Neurodegeneration Research Collection
TypeCollection...primarily involves the malfunction and death of dopamine-producing neurons in the substantia nigra, one...AAV.rTH.PI.Cre.SV40 ready-to-use viral prep useful for dopamine research. CBA-driven, Cre-dependent expression... Next-generation GRAB sensors for monitoring dopaminergic activity in vivo. Sun et al. Nat Methods. 2020...30. Enhance differentiation to mesencephalic dopaminergic (mesDA) neurons using gene knockout . Maimaitili... -
Plasmids for Stem Cell Research
TypeCollection...Fibroblasts Dopaminergic Neurons Lentiviral Human Direct conversion of human fibroblasts to dopaminergic neurons...Biotechnology. 2014 Dec;32(12):1223-30. Zhou Astrocytes Dopaminergic Neurons Lentiviral Human Efficient conversion...conversion of astrocytes to functional midbrain dopaminergic neurons using a single polycistronic vector. PLoS... -
Deisseroth INTRSECT Collection
TypeCollection...Ansorge M, Awatramani R, Kang UJ, Rayport S. 2018. Dopamine neuron glutamate cotransmission evokes a delayed...Amsellem A, Kempf A, Rayport S, Chuhma N. 2020. Dopamine-glutamate neuron projections to the nucleus accumbens...2018. Mapping projections of molecularly defined dopamine neuron subtypes using intersectional genetic approaches... -
Retrograde AAV viral preps
TypeCollection...genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1h Dopamine sensor Li 123308 pAAV-hSyn-GRAB_NE1m...GECO Other Biosensors Glutamate Norepinephrine Dopamine Optogenetics Activators Inhibitors Bidirectional... -
Trimmer Lab NeuroMab Collection
TypeCollection...Botch Rat Mouse IgG2a 114488 Anti-Dopamine D3 receptor [N331/19R] Dopamine D3 receptor Human Mouse IgG2a ...NALCN Rat Mouse IgG2a 177509 Anti-Dopamine D2 receptor [N186/29R] Dopamine D2 receptor Rat Mouse IgG2a 177510...Gamma-protocadherin-B2 Mouse Mouse IgG2a 222156 Dopamine D2 receptor [N186/26R] Dopamine D2 receptor Rat Mouse IgG2a 222157... -
Biosensor AAV Preps
TypeCollection...NIR-GECO Acetylcholine Sensors iAChSnFR GRAB_ACh Dopamine Sensors dLight RdLight GRAB_DA GRAB_rDA GRAB_gDA...Syn HaloCaMP1b EGFP Constitutive 1, 9 Schreiter Dopamine Sensors 111066 pAAV-hSyn-dLight1.1 Syn dLight1.1... -
Genetic Code Expansion
TypeCollection...unnatural amino acids Mammalian TAG Peter Schultz 51401 pAM1 NLL-MetRS E. coli azi-donorleucine (Anl) Bacterial... S. cerevisiae Bacterial Ahmed Badran 209746 pAMC070n1a12 ScTrpRS, Lum1PylRS, MmPylRS S. cerevisiae, Lum1...