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We narrowed to 393 results for: prep

Showing: 121 - 140 of 393 results

  1. Brain Initiative Collection

    Type
    Collection
    ...Plasmid Description PI Viral Preps Addgene distributes ready-to-use viral preps made from plasmids in our... BRAIN Initiative collection of plasmids, viral preps, and antibodies here. Collections...the research community. Browse plasmids , viral preps , and antibodies created with support from the BRAIN...use. Learn more about our viral service ID Viral Prep Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine...

  5. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type...

  7. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...Addgene Browse all AAV vectors Find ready-to-use AAV preps ...STORM imaging, and electron microscopy. Sample preparation for these techniques sometimes makes it more ...retain their ability to be detected after sample preparation better than standard proteins tags like GFP. ...

  10. Hot Plasmids - November 2023

    Type
    Blog Post
    ...subset of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles...doi: 10.1038/s41592-023-01880-5. PMID: 37188954. Preprint available on bioRxiv – doi: 10.1101/2023.03.09.531939...doi: 10.1016/j.cell.2023.05.044. PMID: 37385249. Preprint available on bioRxiv – doi: 10.1101/2023.02.07.527548...doi: 10.1038/s41587-022-01524-7. PMID: 36593408. Preprint on bioRxiv — doi: 10.1101/2021.10.13.463862.  ...

  11. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...some of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles...//doi.org/10.1038/s41477-024-01786-w. (bioRxiv preprint: https://doi.org/10.1101/2024.02.11.579803).  ...

  12. DNA Purification Without a Kit

    Type
    Blog Post
    ... minutes. Additionally, while commercial plasmid prep kits come in discreet sizes (mini, midi, maxi, giga...purification kits. Kit-free alkaline lysis plasmid miniprep Starting material: 2 mL bacterial culture Product...Product: plasmid DNA This kit-free plasmid miniprep protocol from Addgene follows a similar workflow as ...authors estimate that a gravity-based column plasmid maxiprep kit takes 1-1.5 hours to complete, while this ....0007750  Vogelstein, B., & Gillespie, D.A. (1979). Preparative and analytical purification of DNA from agarose...

  13. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...vector preparations, we have used quantitative PCR to evaluate the physical titer of our preps. We often... for ddPCR set-up. Prepare the master mix in a separate area from where you prepare your sample dilutions...

  14. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...genome-containing viral particles that are present in an AAV prep. AAV samples are first DNaseI digested to remove...still necessary. To save time and money, I like to miniprep 5 mL of my AAV transfer plasmid cultures and then...pellet for the remainder of the cultures and later maxiprep only the cultures with intact ITRs. I use a spreadsheet...culture supernatant because it’s time consuming to prepare the 40% PEG solution. PEG takes several hours on.... Rather than investing all of this effort in preparing reagents, I choose to only harvest the cell pellet...separate out contaminants from an impure AAV preparation. 15%, 25%, 40%, and 60% iodixanol solutions are...double-check that the iodixanol gradient solutions were prepared correctly. When aliquoting purified and buffer...

  15. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    ...sent from depositors, bacterial glycerol stocks, prepped DNA ready for our quality control processes - all...The lab prepares bacterial glycerol stocks on Fridays. From each overnight culture, we prepare three sets...sample is barcoded, the lab team takes over and prepares the sample for entry into Addgene’s repository...is contaminated. We use filtered pipet tips and prepare fresh media with antibiotics at each stage. The...Addgene Get help with plasmid transformation, preparation, and analysis from our protocols and molecular...

  16. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...

  17. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...

  18. CRISPR Plasmids - Parasites

    Type
    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Eukaryotic...

  19. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...! Now, let’s head west, intrepid reader! Protein Prep The first step in any western blot is to lyse your...minutes) at 37 °C, if you suspect aggregation in your prep. If your proteins are the wrong size on your gel...

  20. Optogenetics Plasmid Collection

    Type
    Collection
    ... terms. Looking for viral preps? We also offer ready-to-use AAV preparations of many optogenetics plasmids...
Showing: 121 - 140 of 393 results