We narrowed to 603 results for: she
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TypeBlog Post...say your boss approaches your team with a project she needs one of you to lead. At first you might think...identify your transferable skills? PLoS One recently published an article identifying gaps in scientists' transferrable...
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Transferable Skills Guide: Career Planning Resources
TypeBlog Post...Even if you don’t get the specific position he or she originally called about, you can keep in touch and... -
Tips for arabidopsis transformation
TypeBlog Post...Lee is a graduate student at Stanford University. She studies cell fate maintenance in the stomatal lineage... -
Antibiotic Resistance: An Old Solution but a New Problem
TypeBlog Post... bacteriology and drug resistance. Additionally, she is passionate about health equity, science communication...Antibiotics 3. WHO Antimicrobial Resistance Fact Sheet Additional Resources on the Addgene Blog Learn... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog Post...National Emerging Infectious Disease Laboratories. She is interested in genome replication and transcription... the viral mRNAs and minigenome. This can be accomplished in multiple ways: transfecting with an additional... -
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs
TypeBlog Post...proteins for subcellular environment. Currently, she is a SPIRE postdoctoral fellow at the University ...properly? We suggest starting by using the newly published oxidative optimized palette of FPs (moxFPs) [5... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog Post...addition to helping with daily laboratory operations, she also works on improving laboratory efficiency through...samples can be stored in? Different shelves? Different racks on each shelf? Give each container a specific...samples are submitted as bacterial colonies in petri dishes, but close to 80% of samples are received as DNA...large collections. Many companies, such as Thermo Fisher and Micronic, provide 2D barcoded tubes. 1D barcodes...done easily with a barcode reader and an Excel spreadsheet. Start by scanning the sample barcodes into the...and use this ID to name the corresponding Excel sheet.3. Be specific - When assigning a physical location... -
Plasmids 101: Protein tags
TypeBlog Post...Annette Sievers, Ph.D., for her tips and guidance. She is way better at protein purification than he is.... -
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog Post...that control who a female wants to mate with and if she decides to raise their offspring or leave them to... encouraged to not just wait for papers to be published, but to engage in ongoing, real-time dissemination...A paper on stickleback fish science was first published in 1867. It said they were hard to raise because... -
Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names
TypeBlog Post...only a princess who suffered a terrible fate when she pricked her finger on the spindle of a spinning wheel...Groucho Marx, so the gene was aptly named groucho (Fisher and Caudy, 1998). Interested in plasmids that contain... -
Filming Science Videos in the Age of Social Distancing
TypeBlog Post...external video recorder and a mic clipped to her shirt. She rolled several long takes, and we cut it up in post...hopefully they’ll be just as interesting as the finished product for you, the viewer. While you’re here... -
CRISPR-based Genome Editing Systems For Editing in E. coli
TypeBlog Post... Excellence in Molecular Plant Sciences in 2019. She joined Sichuan Normal University and became a lecturer...Originally published Apr 13, 2021 and last updated Aug 12, 2021. This post was contributed by guest bloggers...University, Jieze Zhang, an intern in the Yang Lab, and Sheng Yang, a professor in synthetic biology from the ... optimizing strains with low efficiency. Sheng Yang obtained his Ph.D. from Shanghai Institute ...biomedical applications. Yiwen Zhang entered the Sheng Yang Lab of the CAS Center for Excellence in Molecular... -
Tips for CRISPR Gene Editing in Mice
TypeBlog Post...with a PhD in reproductive biology and genetics. She loves to communicate the latest and greatest in scientific...companies supply gRNA sequences, such as GenScript, ThermoFisher Scientific and OriGene, or you can order them... -
An Integrin Antibody Toolkit from IPI
TypeBlog Post... writer at the Institute for Protein Innovation. She writes about all things IPI, from the scientists ...all bind outside the ligand-binding pocket, their sheer size impeding ligand attachment. The five other ...recommended for use in flow cytometry. Based on the established applications of the original hybridoma monoclonals... -
March for Science
TypeBlog Post...Systems Biology department at Harvard University. She is a scientist with a passion for photosynthetic ... -
SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas
TypeBlog Post...systems in the Sashital Lab, Iowa State University. She is actively interested in science communication and...in Two-Step-SHERLOCK or in a fast, high-throughput manner in one step as One-Pot-SHERLOCK. Two-step-SHERLOCK...sequences, Cas12a activity gets activated. This unleashes indiscriminate ssDNA cleavage activity which cleaves...2020). Detecting coronavirus using CRISPR-based SHERLOCK (Specific High sensitivity Enzymatic Reporter...sensitivity in the zeptomolar range while One-step-SHERLOCK has a sensitivity in the femto-aptomolar range...light illuminator. Approach: Highly similar to the SHERLOCK diagnostic test, this test varies in the sequence...JG, Gootenberg JS, Abudayyeh OO, Zhang F (2019) SHERLOCK: nucleic acid detection with CRISPR nucleases.... -
Using Phosphoserine to Study Protein Phosphorylation
TypeBlog Post...Morgridge Institute for Research in Madison, Wisconsin. She is particularly interested in the intersection of... -
Brain Initiative Collection
TypeCollection... enhancer element Gordon Fishell 83894-AAV2 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent... enhancer element Gordon Fishell 83894-AAV5 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent... enhancer element Gordon Fishell 83894-AAV9 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent... enhancer element Gordon Fishell 83894-AAVrg pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent...hDlx enhancer element Gordon Fishell 83895-AAV1 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP...hDlx enhancer element Gordon Fishell 83895-AAV2 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP...hDlx enhancer element Gordon Fishell 83895-AAV8 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP... -
Is this the right place for me? 8 tactics for choosing a lab
TypeBlog Post...your research and choose wisely. From the Tenure She Wrote blog, Toxic Academic Mentors. What is workplace...the PI? Or do lab alumni leave the field or get pushed out by the PI? What is the management style? How... -
Finding Your Science Policy Path
TypeBlog Post... real-world impacts of health services research. She is particularly interested in driving scientific ...