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We narrowed to 185 results for: INV-E

Showing: 71 - 80 of 185 results
  1. CRISPR Library Amplification

    Type
    Protocol
    ...Maxipreps rely on incremental, ordered cell lysis. E. coli cells are subject to lysis by freeze-thaw if...pre-chilled cuvette. Pulse. Caution Electroporation involves the use of high voltages, please use caution when...
  2. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...Bourges D, Ersing I, Haery L, Harten DeMaio K, Sanders E, Tasissa M, Kostman M, Tillgren M, Makana Hanley L...678 . https://doi.org/10.1089/hum.2019.277 Lecomte E, Tournaire B, Cogné B, Dupont J-B, Lindenbaum P, Martin-Fontaine...and it may also be aligned to plasmids in our inventory containing the suspected contaminating sequence...
  3. Synthetic Biology, Artificial Chromosomes, iGEM & More - Interview with Tom Ellis

    Type
    Blog Post
    ...projects underway in the Ellis lab including yeast and E. coli engineering, microbial cooperation, and antibiotic...team also investigates bacteria and extremophiles for biotechnology and has been directly involved in supervising... through Open Access. Chapter 7/Outro: Tom’s Involvement with iGEM and Addgene  Many thanks to our guest... He returned to academic research in 2005 to investigate synthetic biology at Boston University, working...
  4. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    ...screens." bioRxiv (2018): 356626. 6. Tanenbaum, Marvin E., et al. "A protein-tagging system for signal amplification...Resources Read about CRISPRi and CRISPRa from the NIH E-newsletter ...-wide screens to effectively and efficiently investigate gene function. CRISPR libraries can be used to...
  5. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...us your plasmid information in spreadsheet format, e-mail us at  [email protected] Special cases Please...can distribute your bacterial strains if they: are E. coli that can be used under BSL1 conditions were...allow you to produce large amounts of your insert in E. coli, then this is a Bacterial Expression vector....deposit, associate the plasmids with a Principal Investigator (PI), provide us with your shipping address ...
  6. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    ... UBMTA Chapter 4 - Developing Addgene as a modern e-commerce company and establishing credibility Chapter...Operating Officer. Prior to Addgene, Kenneth was an investment banking analyst at Credit Suisse’s Technology...
  7. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    ...to cancer.   References and resources Milling, L. E., Markson, S. C., Tjokrosurjo, Q., Derosia, N. M.,...pRDA_526 vector to knock out 30 genes putatively involved in CD8+ T cell responses to cancer. The screen...
  8. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...Matho KS, Ieng S, Fouquet S, Dupin E, Benosman R, Chédotal A, Beaurepaire E, Morin X, Livet J. Neuron. 2014...2.1 cleverly combines Cre-mediated deletion and inversion. The Brainbow-2.1 construct can express one of... or M-CFP.) The construct contains two tandem invertible segments, each with two fluorescent protein coding...color possibilities. The remaining cassette can invert as long as Cre remains expressed; once Cre is removed...recombination is required to prevent constant inversion of the construct. Sanes and Lichtman used pronuclear...
  9. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...Thornhill, S. I., Parsley, K. L., Staal, F. J., Gale, R. E., Linch, D. C., Bayford, J., Brown, L., Quaye, M.,...Leavitt, A. D., Robles, G., Alesandro, N., & Varmus, H. E. (1996). Human immunodeficiency virus type 1 integrase... IDLVs were first discovered by researchers investigating HIV integrase mutants in the early 1990s (Leavitt...wish you the best of luck with any experiments involving IDLVs. And remember: You can never trust a lentivirus...of SCID-X1 patients. The Journal of Clinical Investigation, 118(9), 3143–3150. https://doi.org/10.1172/...
  10. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...Anfinan, N., Al-Wasiyah,  mohammad khalid, Huwait, E., Gari, M., & Al-Qahtani, M. (2018). Evaluation of..., Ma, M., Sahay, G., Chiu, A., Bader, A., Langan, E., Siebert, S., Li, J., Greiner, D., & Anderson, D....sense once you realize events are plotted on an invisible Cartesian plane (right.) Each event is plotted...
Showing: 71 - 80 of 185 results