Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 650 results for: CHI;

Showing: 211 - 240 of 650 results

  1. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    ...2017 contest winners:   Chicagoland's Community Biology Lab (@ChiTownBio) and one of our favorite twitter...holiday cheer, and we loved it! Check out their matching "MACK ATTACK!" holiday sweaters. Alex Dainis ...

  2. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    ...business efforts at Addgene as the Chief Financial Officer and Chief Operating Officer. Prior to Addgene... Director of Technology for 7 years, and is now Chief Software Engineer at Ginkgo Bioworks. He also holds...

  3. Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs

    Type
    Blog Post
    ...various cloning methods and tools to construct chimeric cellulases that enable E. coli and Citrobacter...plans for these plasmids have a heavy focus on teaching. He hopes to teach his molecular biology students...Diseases Read About Some of Our Outreach Efforts in China Additional Resources on Addgene.org Learn About...

  5. Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies

    Type
    Blog Post
    ...would be a big deal if it worked out.”- Melina Fan, Chief Scientific Officer “Choose your PhD lab carefully...opportunity.” - Sophia Cheng, Senior Software Engineer “Searching for a new job can be an imposing endeavor.  Break...the place that you are applying.” - Melina Fan, Chief Scientific Officer If there’s a career topic that...

  6. Phage Directory: From Phage Therapy to a Repository of Phage Information

    Type
    Blog Post
    ...therapy, Sacher and Zheng also saw the need to archive phage reagents. If a phage researcher retires, ...likely that the phages are lost if they are not archived. “It’s very sad because it’s years of work,” Sacher... it can also fill this role by cataloging and archiving information on individual phages. Integrating ...

  7. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    ...method that uses iterative staining and chemical bleaching to allow for high-resolution imaging without degrading... Yaniv, Z., Yao, L., Marr, J., Beuschel, R. T., Ichise, H., Gola, A., Kabat, J., Lowekamp, B., Speranza...IBEX: An iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues...

  8. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

    Type
    Blog Post
    ...in the educational sphere, it can range from researching an area, to writing an introduction that gives...mitosis and meiosis in biology. Lots of it is researching the different aspects of the subject you're writing... Keown, a staff writer at the non-profit Fred Hutchinson Cancer Research Center. And make sure to subscribe...

  9. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ...constructing 14 luciferase donor Rluc8-Gα chimeras, four acceptor GFP2-Gγ chimeras, and two untagged Gβ constructs...or drug. In the inactive state, the luminescent chimeric protein Gα-Rluc8 and Gγ-GFP2 will be in close ...

  10. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ...turn up the heat when there is a bad Northeastern chill. Check out Joanne's Reddit AMA  Few of us spend... in this country and we are quick to throw out chipped or imperfect equipment.  We can’t safely recycle...illustration above) and bribed the guys in the hospital machine shop with coffee and donuts to cut the pieces of...

  11. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    ...instability-prone lentiviral vector plasmid in Escherichia coli Stbl3. 3 Biotech 3:61–70. https://doi.org...lentiviral expression vectors using reduced-genomeEscherichia coli. BioTechniques 43:466–470. https://doi.org...cloning strategy for efficient genome editing in Escherichia coli. Microb Cell Fact 19. https://doi.org/10.1186...

  14. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...including a mouse monoclonal (M2) as well as a chimera (rabbit). Myc The Myc tag is a 10 amino acid peptide...still the most prevalent clone used today! Goat, chicken, mouse, or rabbit? Addgene has you covered with...Addgene’s monoclonal antibodies (mouse, rabbit, and chicken) to this tag! GFP  GFP is more than a peptide –...

  16. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...their structure or their interaction with AAVs (Schiller et al., 2018). Exosomal vesicles secreted from...behind these improvements. What have exosome AAVs achieved so far? Exo-AAVs have improved transduction profiles...PMID: 29296848. PubMed Central PMCID: PMC5728288. Schiller, Lara Timantra, et al. "Enhanced Production of...

  17. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...guides – including an excellent series of posts by Joachim Goedhart on the Addgene blog – outlining various...  Simply input these parameters and you’ll find matching FP suggestions. If you’re not sure exactly what...better way to store and represent within-study photobleaching comparisons.  We very much appreciate feedback...

  18. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ... our purposes? In search of simple biological machines R bodies can repeatedly extend and contract...using R bodies: simple, force-generating protein machines that can extend and retract over many microns ...with a fluorescent protein remain functional. Attaching the fluorescent protein fusions allowed us to ...

  19. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...Refactoring and optimization of light-switchable Escherichia coli two-component systems. Schmidl SR, Sheth... at Addgene. Synthetic biology: engineering Escherichia coli to see light. Levskaya A, Chevalier AA, ...Multichromatic control of gene expression in Escherichia coli. Tabor JJ, Levskaya A, Voigt CA. J Mol Biol...

  21. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...can be affected by both autofluorescence and photobleaching. Researchers have long been interested in using... cells. As Nano-lanterns are not subject to photobleaching/phototoxicity, they successfully imaged multiple...applications. For long-term imaging, especially where photobleaching and phototoxicity are a concern, these constructs...

  22. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...instead use a preexisting, conserved cellular machinery to process gRNAs. Hijacking tRNA processing to...plants. Yang was intrigued by the tRNA processing machinery present in almost every living organism. Precursor...PTG constructs in rice protoplasts. Compared to matching traditional gRNA-expressing constructs, transcript...

  23. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ...GFP. The lab tested the base editors in vivo and achieved therapeutically relevant editing efficiencies ...heart, and skeletal muscle. The dosing required to achieve these efficiencies was also between 1013 - 1014...Ace absorbance increases and results in higher quenching of mScarlet emission. Figure 2: Ace-mScarlet...

  24. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...developing assays.  Protocol architecture The basic (very basic!) architecture of the indirect approach is...method relies on multiple secondary antibodies attaching to a single primary antibody, it is sometimes ...

  26. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...what Shoukhrat Mitalipov and his colleagues have achieved, and how the field will move forward from this...circumvent this problem, they co-injected CRISPR machinery with sperm into M phase oocytes, thus allowing...’s important to remember that the study hasn’t achieved any end goal not available with current techniques...

  27. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    ..., Jiang F, Xie J, Kamiyama D. Commun Biol. 2021 Daichi Kamiyama Cerulean Cerulean(1-10) for use with..., Jiang F, Xie J, Kamiyama D. Commun Biol. 2021 Daichi Kamiyama ECFP C155 of ECFP pairs with N173 derived..., Jiang F, Xie J, Kamiyama D. Commun Biol. 2021 Daichi Kamiyama sfCherry2 sfCherry2(1-10) and sfCherry2...

  28. A Look at Addgene's QC Process

    Type
    Blog Post
    ...intended. Sharing unverified plasmids can lead to far reaching consequences, from wasting time and money on optimizing...through the QC pipeline to resolve any issues.  Catching and addressing QC issues Addgene’s QC team has...discrepancies can be caught prior to distribution. Catching these errors prevents future experimental issues...

  29. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...Intermolecular FRET can be experimentally difficult to achieve, because the ratio of acceptor to donor fusions... Before constructing your own FRET probe, try searching PubMed for articles describing the FRET tool that...PMC2396198. 4. Sustarsic, Marko, and Kapanidis, Achillefs N. "Taking the ruler to the jungle: Single-molecule...

  30. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...back into the infants. Almost all patients have achieved persistent immunological reconstitution with a...mutations in the gene RPE65. It is characterized by childhood onset of blindness and was thought to be untreatable...Improvement and decline in vision with gene therapy in childhood blindness." New England Journal of Medicine 372.20...
Showing: 211 - 240 of 650 results