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Showing: 211 - 240 of 782 results

  1. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ... downregulated, examine a protein’s stability or post-translational modifications, or study how a target...learn more about Western blotting check out our blog post The Basics of Western Immunoblotting.) For mass ...details of these methods are beyond the scope of this post.  We hope that this blog has provided you with a...Immunofluorescence View all of Addgene's antibody posts  Resources on Addgene.org: Find plasmids ...

  2. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...they're useful, so we've updated our cover letter blog post to help you write the best one possible!  Should...hiring managers In preparation for updating this post, I took an informal poll of Addgene hiring managers.... and now... you're ready to go! Good luck! This post was originally written by Maria Soriano-Carot in...Cover Letter Guide How to Write a Cover Letter Blog Post from Hubspot ...in the possibility of joining your team as a postdoctoral researcher. Dear Hiring Manager, I am writing...

  3. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...blog Read all of our Plasmids 101 blog posts Read our blog post on 5 factors to consider before choosing...This post was contributed by Jake Watson and Javier García-Nafría from the MRC Laboratory of Molecular...choosing a cloning method Check out all of our blog posts on plasmid cloning Resources on Addgene.org Read...Laboratory of Molecular Biology. Jake Watson is a postdoctoral researcher in neurophysiology at the MRC Laboratory...

  4. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    ...Avaliable at Addgene Read Our Blog Post on Protein Tags Read Other Posts on Fluorescent Proteins Browse Our... This post was contributed by guest bloggers Erik L. Snapp and Lindsey M. Costantini. "You underestimate...proteins within these compartments undergo significant post-translational modifications including glycosylation... addition of N-glycans [9]. Both of these posttranslational modifications can disrupt FP folding rendering...from Oregon Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz...subcellular environment. Currently, she is a SPIRE postdoctoral fellow at the University of North Carolina at...

  5. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...Hybrid Plasmids Read our Blog Post About Yeast Vectors Read our Blog Post About Tagging Yeast Genes ...including protein expression and correct folding, post-translational modification, protein degradation,...cells. If the problem is that your proteins are not post-translationally modified, co-expression of the enzyme...

  6. Summer Fun at Addgene!

    Type
    Blog Post
    ...answering all submitted questions via blog post, which we will be posting in the next few weeks.)  Finally, Addgenies...

  8. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...ready-to-use viral vectors repository? Quite a bit! In this post, we'll share the 20 additions we've made to our ...viral vector services and resources!   This blog post was written by Rachel Leeson and Brian O'Neill.  ...

  9. Addgene’s Blog Gets a New Look!

    Type
    Blog Post
    ...articles from your favorite bloggers Like a blog post from a certain author and looking for more from ...can read more about the Addgenie behind the blog post and find more of their work. Articles from guest...

  12. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...This post was originally written in 2014 by Kendall Morgan and updated in 2022 by Lucie Wilson. Lucie...and RNA therapies. [6]. In 2014, we wrote a blog post on CRISPR’s potential for monogenetic diseases, ...complex genetic diseases. So we’re revisiting this post to look at CRISPR’s potential for use in treating...

  13. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...This post was originally written by Tyler Ford in 2018. It was updated by guest blogger Abhi Aggarwal...made it clear that it’s time to update our iGluSnFR post! Here, we look at the origins of the system and ... that have happened since our original 2018 blog post. The origins of iGluSnFR   iGluSnFR was developed...

  14. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...and 700+ educational resources — including blog posts, protocols, guides, and collections — is a lot of...our content, whether educational resources, blog posts or reagent items.  The technology behind Addgene...such as plasmids and viral preps for catalog, blog posts and protocols for educational resources, and labs...

  15. Components of CRISPR/Cas9

    Type
    Blog Post
    ...nuclease to a user-defined cut site. After reading this post, we hope you will be caught up on much of the major...CRISPR/Cas9 components. Please note that while this post is intended to provide a general overview of CRISPR...guide RNA (sgRNA). Throughout the remainder of this post, we’ll refer to this RNA complex as the “gRNA”. ...

  16. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    ...your connections post. Stay in touch by liking, commenting and re-sharing their posts. LinkedIn users ...Kamens via ASCB Check out more Career Advice blog posts to continue improving your networking and soft skills...sure you have a business card – even students and postdocs. There are many inexpensive/free places to order...the airport. If you see someone traveling with a poster roll, say hello. Keep your cards handy and make...

  17. AAV Packaged on Request is Here!

    Type
    Blog Post
    ...information available for you to dig into. Check our blog post on viral vector production and quality control, ...Packaged on Request webpage, or our announcement blog post to learn more. And our helpful scientific support...

  19. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...blog Browse our blog posts about plant biology Check out our CRISPR blog posts Resources on Addgene....This post was contributed by guest blogger, Robert Orr, who recently received a Ph.D. in Biology and ...non-exclusive gene silencing pathways: transcriptional and post-transcriptional gene silencing (PTGS). In either...silence the target gene(s) either transcriptionally or post-transcriptionally. In plants, different dicer-like...

  20. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...academia would be your school’s post-doc association. And if you’re a post-doc at a school without such ...have been written about this! But this is a blog post, so here are just a few critical features based .... For reasons I don’t understand to this day, my post-doc adviser almost never had lab meetings. In my...Additional Resources on the Addgene Blog Read other posts in the Transferable Skills Guide Management for ...

  21. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...Addgene Read our blog post on the Origin of Replication Browse other Plasmids 101 Posts Get practical molecular... described in our previous Origin of Replication post, DNA replication is initiated at the ori and may... the results. Thanks, stringent regulation! This post was originally written by Jason Niehaus in December...

  22. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    ...blog Browse our blog posts on scientific conferences Read our ECR Toolbox blog post about using social ...This post was contributed by Joe James from Binning Singletons. The sheer scale of a large conference... ASM Microbe in 2019 is written up in mSphere. A post-meeting survey indicated that the program was largely...never went away. While he has frequent bouts with Impostor Syndrome, Joe has found his people through meetings...

  23. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ... This post was contributed by guest blogger Samantha Young. The use of CRISPR/Cas9 for gene editing has...fastest way to use the system in mice? We'll have a post that goes into the mouse genome editing process ...bit more detail in the coming weeks, but, in this post, we will outline a simple method for selecting the...generate gene modified mouse lines. Hopefully this post will help get your in vivo research up and running...

  24. Addgene's Website Update

    Type
    Blog Post
    ...page, and previews for our three most recent blog posts. Finally, you’ll find everyone’s favorite section...at the bottom of the page. Happy surfing!   This post was written by Addgenies Jo Dwyer and Rachel Leeson...

  25. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    ...about pooled library screening, read our recent blog post on Genome-wide Screening Using CRISPR/Cas9. If you...Resources on the Addgene Blog Browse Our CRISPR Posts Learn about Using CRISPR to Generate Knockouts Read...

  26. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    ...Chief Architect Pam McA' Nulty (check out our blog post on CRISPR software to see the importance of programming...Resources at Addgene  Read more Inside Addgene Blog Posts Get Career Advice from Our Bloggers Learn How to...

  27. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...Addgene Blog Browse our Plasmids 101 blog posts Read blog posts on plasmid cloning Read about different ...This post was contributed by Oskar Laur, head of the custom cloning core at Emory University, and Paolo...

  28. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...factors that need to be considered as well. In this post, we’ll touch on antibody storage, buffer considerations...HRP brings us handily to the final topic of this post, the conjugates, or signaling molecules! Please ...protocols - but thankfully this is an Antibody 101 post, so we'll keep it simple. If you follow the two ...

  30. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    ...This post was contributed by guest blogger Jon Chow, an immunology PhD student at Harvard University....University. In my previous two posts, I’ve described the fundamentals of how to work with Drosophila as an experimental...Cre in Drosophila Read Other Genome Engineering Posts Use FLEx Vectors for Site-Specific Recombination...
Showing: 211 - 240 of 782 results