We narrowed to 389 results for: ARIA

1. Career Insights: Technical Support Specialist

   Type

   Blog Post

   ...complain about high background. In many instances, variations of the product’s specific application instructions...

2. Using Video to Share Your Science: We Share Science

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   ...journals: An overview and case study." Journal of Librarianship and Scholarly Communication 2.2 (2014): 2. Additional...

3. CRISPR Activation: A Practical Guide

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   ...Addgene Blog Learn how to Choose the Best Cas9 Variant for Your Next Experiment Find out More about Genome-wide...

4. The Challenges of Cell Culture

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   ...cell lines can become cross contaminated and are invariably much older than those available from biorepositories...

5. Getting the Most from Your Lentiviral Transduction

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   ...applications, stick with them! Changes in such variables as cell density, volume, incubation time, and ...

6. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

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   ...palette of inert green, cyan, yellow, and blue variants that can be used in various combinations. For ...

7. Important Considerations in Optogenetics Behavioral Experiments

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   ...brain region of interest could be low or highly variable without the behavioral test. Another readout may...

8. Plasmids 101: E. coli Strains for Protein Expression

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   ...up a small-scale expression experiment to test variables such as temperature, time, and media conditions...

9. Plasmids 101: The Promoter Region – Let's Go!

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   ...types or organisms, promoters must be similarly variable. Bacterial promoters only work in prokaryotic ...

10. Plasmids 101: Expressing shRNAs for RNA Interference

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    ...19px;font-family: helvetica;","color":"#FFFFFF","variant":null,"font":"helvetica","style":"font-family: ...

11. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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    ...Leavitt et al., 1996), and since then, numerous variants have been developed with different mechanisms ...

12. Antibodies 101: Normalization and Loading Controls for Western Blots

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    ...control won’t be strongly influenced by unexpected variations in any individual protein. This ensures you are...

13. Antibodies 101: Beyond Surface Labeling

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    ...membrane-impermeant. With each cell division, the (invariant) amount of dye is reduced per cell (Figure 4a)...

14. Antibodies 101: Introduction to Gating in Flow Cytometry

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    Blog Post

    ...parameters the machine uses to differentiate between variations in those factors. To understand the principles...

15. An Integrin Antibody Toolkit from IPI

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    Blog Post

    ...are chimeric antibodies composed of rat or mouse variable regions fused with rabbit constant domains. All...

16. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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    ...the Addgene Blog Learn about High Fidelity Cas9 Variants How to Design Your gRNA Delivering CRISPR Ribonucleoproteins...

17. Harnessing Bacterial Toxins for Allelic Exchange

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    ...there is also a large amount of user-to-user variability, and in some cases, counter-selection escape ...

18. Five Popular Model Organisms, Part 2

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    ... Arabidopsis include understanding of allelic variation between different plant species and metabolic ...

19. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

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    Blog Post

    ...expression with an overlapping AAV since both of these variables influence whether cells will be co-transduced ...

20. CRISPR 101: Validating Your Genome Edit

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    ...Pinello L. CRISPRitz: rapid, high-throughput and variant-aware in silico off-target site identification ...

21. Multiple Plasmids at a Low Price: Kits from Addgene

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    Blog Post

    ...their PUF Assembly Kit. The kit contains all the variant PUF segments required to bind any 8 base RNA sequence...

22. Hot Plasmids: Spring 2025

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    ... mutants, identifying the two  best-performing variants as iGluSnFR4f (fast deactivation) and iGluSnFR4s...

23. CRISPR 101: Off-Target Effects

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    ...of optimization which have led to more specific variants. HypaCas9, sSpCas9(1.1), SpCas9HF1, and evoCas9...

24. Viral Vectors 101: Optogenetic Tools

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    ...responsive to light in combination with retinal) variations designed to give researchers flexibility in using...

25. Antibodies 101: Multiplex Immunofluorescence

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    ...possibilities, and there are always new and improved variations you could try. We hope this post helped you start...

26. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

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    ...30-60%. Quadros et al. attribute much of this variation to differences in guide RNA efficiency. For example...

27. Technologies Enabled by NanoLuc® Luciferase

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    ...PMC4942401. 5. Susuki, K., et al. (2016) Five colour variants of bright luminescent protein for real-time multicolour...

28. Antibodies 101: The Basics of Western Blotting

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    ...assessed from a single western.  There are several variations of the protein transfer step including wet, semi-dry...

29. Important Considerations When Using AAVs

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    ...types. In the CNS, it has been shown that there is variable transduction of oligodendrocytes, microglia, astrocytes...

30. A History of Genome Engineering in Popular Culture

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    ...curing diseases caused due to single nucleotide variations in the genome.    Due to the rise of CRISPR in...

31. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

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    ...being used in any genetic background, but require variable amounts of experimental manipulation and screening...

32. Adeno-associated virus (AAV) Guide

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    Guide

    ...vectors. AAV Types Serotypes AAV serotypes are AAV variants that differ in their tropism — that is, the types...determined by naturally occurring differences in the variable regions of the virus capsid. Thirteen human and...vectors for in vivo applications. Other Engineered Variants Hybrid capsids Hybrid capsids are engineered capsids...successfully employed to engineer new rAAV capsid variants, such as systemic capsids. A systemic capsid is...also been employed to engineer an rAAV serotype variant able to display neuronal retrograde transport, ...D. V., & Karpova, A. Y. (2016). A designer AAV variant permits efficient retrograde access to projection...

33. Cancer and the Immune System: Deciphering the Relationship

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    ..."Specific recruitment of regulatory T cells in ovarian carcinoma fosters immune privilege and predicts...

34. Special Delivery: Fluorophore Targeting for FRET Studies

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    ...genetically-encoded FRET donors (Fig. 1). Scores of FP variants have been created, thereby enabling a broad spectrum...

35. CRISPR 101: Cytosine Transversion Editors

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    ...they expanded CGBE target scope by creating BE variants with other Cas9s that recognize different PAMs...

36. Modular Cloning Guide

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    Guide

    ...constitutive promoters, T7 expression, RBS strength variants, synthetic terminators, protein purification tags...including 11 inducible promoters, 16 RBS strength variants, 8 tag-compatible RBSs, 2 secretion tags, 6 CDSs...

37. Chemogenetics Guide

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    Guide

    ...CTZ) or chemical variations thereof (hCTZ), or furimazine (Fz) or chemical variations thereof (FFz, CFz...

38. Gamma-Retroviral Vector Guide

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    Guide

    ...vectors are produced in just a few days. Another variant, Phoenix-gp, contains only gag-pol and enables ...

39. Plan Your Experiment

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    Guide

    ... window from the PAM sequence, and base editor variants often have different optimal editing windows. ...