...the RNAi transgene, followed by a fluorescence readout of RNAi activity. As a plant researcher, the traditional...

...electrophoresis. Surveyor™ and T7E1 have been widely adopted due to their relative simplicity and low cost. ...

... notable in recent years. A variant of V-U, paradoxically called V-K, is a CRISPR-associated transposase...

...together, are able to give you the appropriate readout. A common method of labeling proteins is to fuse...

...of interest, which can affect your experimental readouts. Be aware that in any version of this approach...

...understand variation due to technical error.  Plate readouts can be affected by plate type or tubes, sealing...

...these instructions to prepare the PSU ladders! Rosado, et al. Autophagy. 2008. PubMed PMID: 18094608 ...

...., Greenbaum, A., Ravi, N., Wu, W. L., Sánchez-Guardado, L., Lois, C., Mazmanian, S. K., Deverman, B. ...

...examine the mechanisms behind autophagic pathways. Rosado, et al. Autophagy. 2008. PubMed PMID: 18094608 ...

...Theunissen, T. W., Rangarajan, S., Shivalila, C. S., Dadon, D. B., & Jaenisch, R. (2013). Multiplexed activation...Communications , 9 (1). PMID: 30082838 Maddalo, D., Manchado, E., Concepcion, C. P., Bonetti, C., Vidigal, ..., Cogan, J. Z., Replogle, J. M., Adriaens, C., Ramadoss, G. N., Shi, Q., Hung, K. L., Samelson, A. J.,...Partridge, E. C., Newberry, K. M., Smith, S. B., Meadows, S. K., Roberts, B. S., Mackiewicz, M., Mendenhall...

...activated by the synthetic ligand spiradoline, however, spiradoline exhibited off-target effects in vivo...

...NHEJ repaired DSBs): Provides a semi-quantitative readout of the percentage of alleles that have been mutated...

...stimulated. Channelrhodopsin-2 (ChR2), the first widely adopted optogenetic tool, is also a blue light activated...

...Sample preparation, signaling excitation, and assay readout vary widely depending on individual assay conditions...