We narrowed to 186 results for: Coli

...Bioworks to distribute SARS-CoV-2 plasmids for E. coli , yeast, and mammalian expression, and spike ...

...and activation). Genome support for bacteria ( E. coli, B. subtilis ), yeast ( S. cerevisiae ), worm ( ...

...after each transformation and/or use different E. coli strains with higher transformation efficiency. If...

...construct can be easily expressed and purified from E. coli, retains important functional properties, and is...

...transforming a backbone phagemid vector into an E. coli strain that is deficient in dUTPase and uracil deglycosidase...

...PCR reaction, no ligation is required since the E. coli you transform your PCR products into will efficiently...

... of E. coli strains below and recommend checking out Addgene’s blog posts about common E. coli lab strains...Elements Types of Plasmids Molecular Cloning Common E. coli Strains Common Antibiotics DNA Sequencing Sanger...For instance, most plasmids are replicated in E. coli and are relatively small (∼3,000–6,000 base pairs.... When a plasmid exists extrachromosomally in E. coli , it is replicated independently and segregated ...Common Bacteria Strains for Propagating Plasmids E. coli are gram-negative, rod-shaped bacteria naturally...many different naturally occurring strains of E. coli , some of which are deadly to humans. Most of all...all common, commercial lab strains of E. coli used today are descended from two individual isolates, the...

...constructs constrained only by the ability of E. coli to maintain the final plasmid after transformation...purification tags and fluorescence proteins for use in E. coli for a variety of applications. EcoFlex MoClo Extension...backbones, and 6 destination vectors for use in E. coli for a variety of applications. CIDAR MoClo Parts...combinatorial design and expression tuning in E. coli . CIDAR MoClo Extension, Volume I Bacterial Expression...ubiquitination cascades of different organisms in E. coli . MetClo DNA Assembly Kit Bacterial Expression Christopher...and FBF (PUF) homology domain, for mammalian or E. coli expression. T2A Split/Link TurboID Kit Mammalian...

...with E. coli araBAD promoter Forward pBAD Reverse GATTTAATCTGTATCAGG For vectors with E. coli araBAD promoter...

...post: Synthetic Photobiology: Optogenetics for E. coli. Schmidl, et al. ACS Synth Biol. 2014 Nov 21...aggregation propensity in four expression systems: E. coli, wheat germ, HeLa, and Leishmania (Gagoski, et al... replication increases plasmid copy number in E. coli. The authors used pXPG to study the human GM-CSF... F30-2xdBroccoli are available, as well as 5S RNA F30-2xdBroccoli fusion and pET28c-F30-Broccoli controls...mammalian cells with Broccoli Vegetable tags, including Spinach, Spinach2, and Broccoli, can benefit your...scaffold allows insertion of two dimeric Broccoli tags (2xdBroccoli) to further enhance fluorescence of the...controls. Don’t just eat more broccoli, use Broccoli tags in your experiments too! Filonov, et al. Chem...

... RSMs? In our study (1), we implement RSMs in E. coli with two plasmids (Figure 3 below): Input Plasmid...

...luciferase gene allow for increased copy number in E. coli and expression of luciferase in the cytoplasm of...

...created by directed evolution of the Escherichia coli TadA, a tRNA adenosine deaminase. Like cytosine ...the DNA and replaces it with another base. In E. coli , this strategy yields C to A base edits, whereas...harnessed for antiviral mechanisms in bacteria ( E. coli ). Like the other Cas enzymes, the Cascade-Cas3 .... (2018). Genome-wide CRISPR-dCas9 screens in E. coli identify essential genes and phage host factors....

...recombination persists even in recombinase deficient E. coli and under conditions used to minimize recombination...

...Engineering production of functional scFv antibody in E. coli by co-expressing the molecule chaperone Skp. Front...

... adapted an antibiotic resistance operon from E. coli : the tetracycline repressor protein (TetR) and ...

...demonstrated in 2005 that proteins fused with E. coli dihydrofolate reductase (eDHFR) could be labeled...

...prime editors with RT domains derived from the E. coli Ec48 retron RT and the S. pombe Tf1 retrotransposon...

...Bacterial Expression Construct SpCas9 Inhibition (E. coli) Mammalian Expression Construct SpCas9 Inhibition...

...AdEasier-1 cells (Addgene #16399) , which are BJ5183 E. coli cells containing pAdEasy. pAdEasy is a ~33 kb adenoviral...exposure to an antigen. AdEasier-1 cells BJ5183 E. coli cells containing the pAdEasy-1 packaging plasmid...