We narrowed to 497 results for: LARGE

...(~150 kDa) and Y-shaped because it contains two large heavy chains and two small light chains that stick...optimally binding sybody, you want to start out with a large and diverse pool of sybodies that hypothetically...because it has the advantage of starting out with a larger initial starting library size (up to 1012 members...

...already a packaging capacity larger than AAV vectors. This capacity is large enough to carry the Cas9 gene...compared to other viral vectors, AdVs can provide a large transgene packaging capacity. For instance, with...

...self-inactivating, which is achieved by deleting a large portion of the 3'LTR in the transfer plasmid. The...through insertional mutagenesis. These risks are largely based upon the vector system used and the transgene...

...cartridges can place the spent cartridge in the large cardboard box in the copy area. These spent cartridges...

...only one functional copy of the p53 gene causes a large reduction in tumor suppression activity, leading...

...pathways and represent the largest family of human drug targets. To enable large-scale profiling of many ...by endogenous GPCR. Example applications include large-scale pharmacological profiling or interrogation...

...lentiviral-based CRISPR libraries available at Addgene. The large packaging capacity and stable expression of lentiviral... vivo CRISPR screens. AAV vectors have played a large role in these advancements, due to their many benefits...of molecular pathways. These screens have gotten larger, easier, and more comprehensive thanks to the consistent...

...plasmids in the library.  Spinfection If you have a large number of cells to transduce, you may want to consider...pooled libraries can be intimidating and require a large amount of experimental work, but with this guide...protocol. After functional titer is determined, larger lentiviral preps can be used for screening-scale...

...fusion proteins be in close proximity and present in large enough quantities (and the correct stoichiometric...nature of BiFC also makes it easier to generate a large number of constructs for high-throughput screening...

...the University of Goettingen, and several other large research institutions. The following graphic depicts...institutional and lab servers specifically designed to hold large amounts of data. Although it may be useful to selectively...

...novel drugs. Commercial Affairs teams make up a large and diverse group of professionals running the marketing...challenging. For instance, the client might need to get a large amount of market research done to inform a go/no-go...

... fused to a dimerizing FP has potential to form large stable polymers of dimers. 3) FRET biosensors that...dimerizing FP is fused to the POI. These structures are large (microns), bright, non-physiological, and difficult...

...which is formed by a complex of two proteins: the large (L) catalytic protein and its cofactor VP35. VP30...necessary control elements for each would be relatively large. Cloning the viral genes in this plasmid would be...

...datasets are too large to transfer to a journal repository, they are likely too large to be retrieved ...their own data, with some smaller labs feeling that larger, better resourced, groups would harvest and reanalyze...

... chemical synthesis of these molecules. To make large amounts of pure product in an affordable way, researchers...that can be assembled into individual TUs or very large (up to 20!) multi-TU constructs (Moore et al., 2016...needed in mammalian cell types, and comes with a large library of common parts to get you started quickly...build genetic circuits and would benefit from a larger library of parts but don’t need to add tags to ...multiplexed CRISPR, and chromosomal integration of larger expression cassettes (Shaw et al., 2023). Those...

...ambitious projects that require epitope tagging of a large cohort of genes (e.g. tagging genes representing...success of the epitope tagging experiment depends largely on how efficiently these components are delivered...immense possibilities to delineate the function of large protein cohorts in diverse mammalian cell types....

...properties of the final product, particularly in large animal studies. Endotoxin contamination is minimized...

... a patent counsel and ran a group at two fairly large companies, Genzyme and Biogen. They’re very different...

... the user in different formats. This can have a large impact on downstream results. Examples: “HTML”, ... agnostic”, “model organisms”, “small genome”, “large genome”, and “organism biased scoring”. Trends: ... CRISPR design (I’m talking screens and/or very large genomes), then it is worthwhile buying a dedicated...

...offering temperature-sensitive materials, we noticed a large percentage of these shipments arrived in India at...expertise in a specific country can quickly reduce a large number of barriers. Though we haven’t solved every...