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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 469 results for: LARGE

Showing: 141 - 160 of 469 results

  1. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...ambitious projects that require epitope tagging of a large cohort of genes (e.g. tagging genes representing...success of the epitope tagging experiment depends largely on how efficiently these components are delivered...immense possibilities to delineate the function of large protein cohorts in diverse mammalian cell types....

  2. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ... chemical synthesis of these molecules. To make large amounts of pure product in an affordable way, researchers...that can be assembled into individual TUs or very large (up to 20!) multi-TU constructs (Moore et al., 2016...needed in mammalian cell types, and comes with a large library of common parts to get you started quickly...build genetic circuits and would benefit from a larger library of parts but don’t need to add tags to ...multiplexed CRISPR, and chromosomal integration of larger expression cassettes (Shaw et al., 2023). Those...

  4. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ... pathway or measurement of a biomolecule while largely preserving spatial and temporal cellular processes... Engineering of NEMO as calcium indicators with large dynamics and high sensitivity. Nat Methods. 2023...receptor Glutamate receptor exocytosis and spine enlargement during chemically induced long-term potentiation...

  6. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...offering temperature-sensitive materials, we noticed a large percentage of these shipments arrived in India at...expertise in a specific country can quickly reduce a large number of barriers. Though we haven’t solved every...

  7. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...deliver larger cargo such as base editors and prime editors. While using AAV to deliver large transgenes... with split AAVs. In general split AAVs break a large transgene in two pieces and package each piece into...

  8. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...reconstitution via mRNA trans-splicing. By splitting a large gene into smaller fragments, delivering them by ...prime editing, and delivery of disease-relevant large proteins. They demonstrated REVeRT in various contexts...

  9. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...Reddit. Here are some reasons why: 1. Reddit has a large and active community of people interested in science... moderators did not delete or suppress were the large number of scientist posters with serious career ...

  11. Fueled by Coffee at #SfN14

    Type
    Blog Post
    ...open-access. Though it is time for me to refill my large coffee cup and get back to our booth, I will have...

  12. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ...individual plasmid within a single bacterial cell. Large plasmids usually have a low copy number (approximately... other hand, smaller plasmids can be present in large numbers, 50 or more per cell and have a high copy...usually want to start 2 mL in a falcon tube, but for larger preps you might want to use as much as a liter ...

  13. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...colonies that are comprised of cells with a large nucleus, large nucleoli, and little cytoplasm. Positive...other methods. This could be a reflection of the large size of the plasmid or silencing of the plasmid ...oncogenes, such as Myc. Viral vectors also have the largest genomic footprint of iPSC generating methods due...infect dividing and nondividing cells and have a larger cloning capacity (8-10 kb) than other retroviral...transfection efficiency is cell-type dependent and larger plasmids have lower rates of transfection. Minicircles...

  18. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...counter-selection. Anecdotally, there is also a large amount of user-to-user variability, and in some ...modification of bacterial genomes. It can accomplish large deletions, insertions, and even precise point mutations...

  19. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ...companies now sell fast digest enzymes that can digest large amounts of DNA in as little as 10 minutes, but check...Additionally, if your final product is going to be very large (>10kb) you might want to use electro-competent ..., the DNA migrates toward the anode (+ charge). Larger fragments of linearized DNA migrate slower than...purification you can do is a miniprep, but if you need larger quantities of DNA, you’ll need to do a midiprep...

  20. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...genes for deoxyribose synthesis Allows uptake of large plasmids hee   High electroporation efficiency...Chloramphenicol High competency cloning and propagation of large plasmids, ligated DNA, and libraries, nalidixic ...
Showing: 141 - 160 of 469 results