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We narrowed to 1,020 results for: NTS;

Showing: 141 - 160 of 1020 results

  1. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...transcriptase. Engineering of the PE enzyme and related components have helped to improve the efficiency of editing...

  2. AAV Molecular Tools

    Type
    Collection
    ...encode tools that can be used for neurophysiology experiments, including neuronal connectivity labeling. ID...

  4. Ras Pathway

    Type
    Collection
    ... to find available plasmids for the gene. For components with multiple subunits or isoforms, individual...

  5. Control AAV Preps

    Type
    Collection
    ...Promoter CAG CaMKIIa CMV Dlx EF1a/nEF GFAP and variants Synapsin TBG Other Fluorophore/Tag Green Red None...

  6. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...initial version of EvolvR has an editing window of ~17 nt from the nick site, which was expected since the ...generating novel genetic variants in bacteria. Read on to learn about the key components of EvolvR and its potential...library used. EvolvR: key components The EvolvR system has two key components: the error prone PolI3M that...scale by screening diverse libraries of genetic variants. Several methods for generating mutant libraries...protein-protein interactions, investigate non-coding segments of the genome, or engineer microbes to perform...to start EvolvR-ing your targeted mutagenesis experiments? Find EvolvR plasmids at Addgene! References...

  7. Brain Armamentarium

    Type
    Collection
    ...is developing a collection of molecular genetic reagents to gain access to many different brain cell types...

  8. Retrograde AAV viral preps

    Type
    Collection
    ...Karpova lab. Results for our quality control experiments can be found on the material pages for viral ...

  9. Caltech Systemic Capsids

    Type
    Collection
    ...GRE or transgenic animal For PNS applications in rodents and NHPs: Broad tropism - AAV-MaCPNS2, AAV-PHP....

  10. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...doi.org/10.1016/j.cell.2013.08.021 Waters CA, Strande NT, Pryor JM, Strom CN, Mieczkowski P, Burkhalter MD...regardless of end structure, and dictate the major events of the pathway: Broken ends are recognized by ... probably accounting for less than 5% of repair events. However, the products of accurate repair are easily...Yan et al., 2020). NHEJ can also be engaged by variants of the canonical Cas9 approach. A pair of CRISPR...

  12. Hot Plasmids - October 2020

    Type
    Blog Post
    ...introduce the small wrmScarlet11 which is less than 60 nt in length to tag the target protein. The team validated... without the constraints of antibody labeling. This opens the door to in vivo experiments such as tracking...composed of two FP fragments that are not fluorescent on their own. When the two fragments come together, ...

  13. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...default nucleotide database “Nucleotide collection (nr/nt)” as it contains a composite of GenBank, EMBL, DDBJ...of the different BLAST programs, databases, and elements of the BLAST search pages. At Addgene, we use ...not as fast as megablast, but can return longer alignments to compare with your sequencing trace file. Unlike...be your optimal choice. For difficult sequence alignments that BLAST is unable to handle, Clustal is our...frequent choice for pairwise or multiple sequence alignments of nucleotide or protein sequences. We also use... to confirm your plasmid sequencing results or comments on our suggestions? Share your thoughts here to...

  14. Brain Initiative Collection

    Type
    Collection
    ...identified by depositors and/or when BRAIN Initiative grants are noted in publications associated with Addgene...

  15. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ... The schematic to the right shows the separate elements of the R-mAb expression plasmid involved in coexpression...ER membrane. PCR-mediated fusion of these three elements is followed by their insertion into the p1316 ...

  16. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ...mutations, also has a strict editing window about 15 nt upstream of the PAM. Even if there’s a PAM near your...for you in the comments section! References Hu, Johnny H., et al. "Evolved Cas9 variants with broad PAM...work, there are other options. Commonly used Cpf1 variants, with a  5’ TTTV PAM, improve targeting in AT-...respectively. Researchers also engineered SpCas9 variants with alternative PAMs like NGAG and NGCG and SaCas9...phage-assisted evolution to generate and select for Cas9 mutants with PAM flexibility. In this system, the mutagenesis... in the pool. According to this logic, ω-dCas9 variants recognizing multiple PAMs should have a fitness...promoting survival of only the broadest-targeting variants. For the final round of evolution, they used a...

  17. Design Tips for Prime Editing

    Type
    Blog Post
    ... binding site, starting with a length of about 13 nt (Anzalone et al., 2019). Primer binding sites with...Edit the PAM along with your intended edit. This prevents the Cas9 nickase from re-binding and nicking the... al., 2019). Figure 4: Editing the PAM prevents the prime editor from re-engaging with DNA it ...feel free to share your favorite software in the comments below! References and Resources References Anzalone...prime editing systems by manipulating cellular determinants of editing outcomes. Cell, 184(22), 5635-5652...

  18. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    ... 1A, top):  A CRISPR RNA (crRNA) comprising a 32 nt spacer sequence specifying the target site. Four ...Center. DNA transposons are ubiquitous genetic elements capable of spreading within and between genomes...was named INTEGRATE (Insertion of Transposable Elements by Guide RNA-Assisted Targeting) by the Sternberg... a Type I-F CRISPR-Cas system. The four major components required for RNA-guided DNA integration are (...sequences (R and L ends).  Figure 1: (A) Components involved in each type of CRISPR-Transposon system... proteins (TnsB and C). While requiring fewer components for RNA-guided DNA integration, Type V-K systems... promoters were used to express the necessary components in E. coli (Klompe et al., 2019). Recently, the...

  19. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ... in vivo, the Jaffrey lab used SELEX to find a 98 nt RNA aptamer that would bind the fluorogen and increase...applications, including detection of environmental pollutants, drug discovery, and disease diagnosis. As biosensors...instead of protein-based fluorophores, including: Environments with higher temperatures, since proteins can...electrochemical sensors for emerging environmental pollutants.” Front Chem. 2014; 2: 41. PubMed PMID: 25019067...the Addgene Blog Tips for Using FRET in Your Experiments Learn more about GFP Controlling Protein Activity...

  20. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...https://doi.org/10.1126/science.1138389 Le TTH, Tran NT, Dao TML, Nguyen DD, Do HD, Ha TL, Kühn R, Nguyen... veteran and new Rett researchers can find the reagents and resources they need to cure Rett Syndrome....cardio-respiratory dysfunction, distinctive hand movements, and show autistic-like behaviors.  The role of...
Showing: 141 - 160 of 1020 results