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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 497 results for: RAP

Showing: 141 - 160 of 497 results

  1. 6 Tips for Grant Writing

    Type
    Blog Post
    ...perfectly reasonable paragraph unintelligible to a lay audience. My lay paragraph typically includes a...the best place in the world for your research to rapidly progress. Need Help Building Your Scientific Network...kindly friends and family to read my non-expert paragraph and point out words or sentences that are unclear...

  2. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...article ultimately reported the development of a rapid design and prototyping pipeline that can take pathogen...the Zika virus genome with Addgene to facilitate rapid sharing of these tools with the Zika virus research...other critical needs and to further develop our rapid prototyping and sensor assembly pipeline for future...sustainability.   References 1. Pardee, Keith, et al. "Rapid, Low-Cost Detection of Zika Virus Using Programmable...

  3. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type
    Blog Post
    ...models for developmental processes and promising therapeutic tools. The two unique characteristics that define...cells can be used for different research and therapeutic purposes. Both types have their advantages and...of post-transplantation rejection when used therapeutically High Low (if ASCs are isolated from the patient...directly from a patient’s adult cells when used therapeutically, the chances of post-transplantation rejection...

  4. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...sensitization and might open new ways for a novel therapeutic approach against drug addiction. Anxiety tests...pro-inflammatory cytokine interleukin IL-1 beta in the dorsal raphe nucleus (DRN), a region thought to be involved in... medial prefrontal cortex Drd1 neurons produces rapid and long-lasting antidepressant effects." Nature...., Alison V. Roland, and Tracy L. Bale. "Dorsal raphe neuroinflammation promotes dramatic behavioral stress...

  5. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type
    Blog Post
    ...One of the biggest barriers to gene therapy treatment of central nervous system (CNS) diseases is the...Transport across the Blood-Brain Barrier." Molecular Therapy 27.5 (2019): 912-921. PubMed PMID: 30819613. Hordeaux...AAV-PHP. B are limited to C57BL/6J mice." Molecular Therapy 26.3 (2018): 664-668. PubMed PMID: 29428298 PubMed...dose–response and comparison to AAV PHP. B." Gene therapy 25.5 (2018): 392. PubMed PMID: 30013186. Matsuzaki...

  6. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...toxins to facilitate efficient recombineering (Khetrapal et al., 2015). They created vectors for Lambda...sacB cassette with one of three toxins from the Khetrapal paper. We selected the three toxins so that at...control of a rhamnose-inducible promoter, though Khetrapal et al. also demonstrated that the arabinose promoter... 23360965. PubMed Central PMCID: PMC3748948.  Khetrapal, Varnica, et al. "A set of powerful negative selection...

  7. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...they circulate in the blood, rather than being geographically constrained to the brain. In cases where it...underexplored sites on the AAV capsid. Molecular Therapy - Methods & Clinical Development, 26, 331–342. ...for Robust CNS Transgene Expression. Molecular Therapy - Methods & Clinical Development, 15, 320–332. ...-PHP.B Are Limited to C57BL/6J Mice. Molecular Therapy, 26(3), 664–668. https://doi.org/10.1016/j.ymthe...Transport across the Blood-Brain Barrier. Molecular Therapy, 27(5), 912–921. https://doi.org/10.1016/j.ymthe...Non-human Primates Compared with Mice. Molecular Therapy, 27(11), 2018–2037. https://doi.org/10.1016/j.ymthe...

  8. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...are released in each synaptic event and they are rapidly taken up by postsynaptic receptors and transporters...sensitivity. iGluSnFR4f is well-suited for imaging rapid synaptic events such as in the somatosensory cortex...much to learn about their myriad functions and therapeutic potential. Our partners at the Institute for ...critical — factor for successful experiments or therapies. Using AAV vectors with enhancers that drive cell-type-specific...

  9. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    ...of the DA neurons produce behavioral and electroencephalography evidences of arousal in mice previously...enabled the drawing of a detailed and functional topography of the neural circuit architecture of the brain...exceeding the carrying capacity of AAV vectors." Gene Therapy for Neurological Disorders. Humana Press, New York...26135556. Hudry, Eloise, and Luk H. Vandenberghe. "Therapeutic AAV gene transfer to the nervous system: a clinical...

  10. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...software could also be used as a simple tool to rapidly find one or a few gRNAs suggested to be effective...appropriate phenotypic readout, similar mapping could be rapidly performed by targeting all potential components...identified for each gene. This file can be used to rapidly get an overview of the list of gRNAs generated....screen identifies genetic vulnerabilities and therapeutic targets in acute myeloid leukemia." Cell reports...

  11. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...Assembly Standard and New E. Coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional....2020.100135. Fonseca, João Pedro, et al. “A Toolkit for Rapid Modular Construction of Biological Circuits in Mammalian...Extensible Mammalian Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors...Abby V., et al. Modular Vector Assembly Enables Rapid Assessment of Emerging CRISPR Technologies. Cold...

  12. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

    Type
    Blog Post
    .... TALENs - Genome engineering methods improved rapidly in 2011, from zinc finger nucleases to transcription... 10 years of plasmid sharing? Check out this infographic created by our own Addgenie, Laura Veckerelli...Veckerelli. Click on the picture below to see the full graphic.        ...

  13. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    ...become resistant to drugs, particularly targeted therapies,” said Kris Wood of Duke University Medical Center...identify those that confer resistance to drugs,” in a rapid, straightforward, and scalable manner, Wood said... cancer therapy...

  14. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    ...reason this research has been able to progress so rapidly is scientific sharing. Already we’ve seen articles...and data associated with COVID-19 research have rapidly been developed. We asked the individuals behind...is giving Rutgers investigators the agility to rapidly initiate complex research projects that they would...

  15. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    ...Adeno-Associated Virus (AAV) as a Vector for Gene Therapy. BioDrugs 31:317–334 . https://doi.org/10.1007/...Improving the Efficacy of AAV Vectors. Molecular Therapy - Methods & Clinical Development 12:248–265 . https...Vector Production and Transduction. Human Gene Therapy 31:499–511 . https://doi.org/10.1089/hum.2020.069...

  16. Sensing Neuronal Dopamine

    Type
    Blog Post
    ... is relatively slow and is not suited to detect rapid changes in DA concentration. To address this, researchers...Studies on the kinetics of these sensors showed rapid response (60 or 130 ms on and 0.7 or 2.5 s off, ... genetically encoded fluorescent sensor enables rapid and specific detection of dopamine in flies, fish...

  17. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...into the clinic as well. The first sdAb based therapeutic was approved in 2018, with many others in clinical...clinical trials (Jin, et al. 2023). In addition to therapeutics, there is a lot of excitement around using sdAbs...Nanobodies: A Review of Generation, Diagnostics and Therapeutics. Int J Mol Sci 24:5994. https://doi.org/10.3390...

  18. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

    Type
    Blog Post
    ...preclinical and clinical data about a disease or therapeutic Side-by-side comparison of the same experiment...reproducible. For an example, see our ReFigures on the therapeutic use of anti-CD47 antibodies in immuno-oncology...where the impact of teaching and doing science is rapid, concrete and open to innovation by all.  Additional...

  19. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...thicker peptidoglycan layer, gram positive bacteria trap the crystal violet stain within their membrane, ... added, it dehydrates the peptidoglycan layer, trapping the large dye complex created by the interaction...in marine biology and minoring in studio arts photography.  References and resources References Silhavy...

  20. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...more effectively used in a technology or as a therapeutic.  A (very) brief history GCE came onto the synthetic...synthetase introduced to the bacteria using plasmids. Rapid expansion occurred, with hundreds of new ncAAs and... any number of experiments with. The catch The rapid expansion of GCE led to the creation of many new...
Showing: 141 - 160 of 497 results