Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 682 results for: RON

Showing: 141 - 160 of 682 results

  1. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ... Jason Moffat at the University of Toronto has created the Toronto KnockOut (TKO) CRISPR Library. This...for neuronal systems. To develop a new single-component tool for magnetogenetic control of neurons, the...sequences outside of their endogenous chromatin environment and relies on the fact that enhancers function...J Genet Genomics. 2016. PubMed PMID: 26887506 Toronto KnockOut (TKO) CRISPR library In order to identify...Natl Acad Sci USA. 2016. PubMed PMID: 26884157 Aronshtam, et al. Nucleic Acids Res. 1996. PubMed PMID: ...but turns more red in lower (e.g. vacuolar) pH environments that inactivate SEP and leave only DsRed to ...the Co-IP compared of a control Co-IP indicates stronger interaction. The system has been demonstrated ...

  2. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...inducible degron (AID) system in C. elegans By Alyssa Cecchetelli The auxin inducible degron (AID) system...Tracking cell-cell interactions New auxin inducible degron system for C. elegans New CRISPR plasmids New items...cell types including endothelial cell-pericyte, neuron-astrocyte, and cancer-stromal cell pairs.   ...   Schematic showing the auxin-inducible degron system. Image from Ashley et al., 2021.   The...spatiotemporal expression pattern and an auxin inducible degron (AID) sequence attached to your protein of interest...presence of auxin, this TIR1 complex binds to the degron sequence ultimately leading to the degradation ...the AID* (minimal 44 amino acids auxin inducible degron sequence) tagged proteins. Degradation of your ...

  3. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type
    Blog Post
    ...activity of neurons on or off using pulses of light. By activating a neuron or set of neurons, you can see...activate a set of neurons, maybe we could treat patients suffering from Parkinson's, chronic pain, deafness...independently control the activity of two populations of neurons at once. Addgene spoke to Boyden about this latest...contributes to behavior or pathology, and by disabling neurons you can figure out what they are necessary for....examples where you want to activate two sets of neurons and see how different parts of the brain or the...

  4. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...Much later, it was shown that putting the operon under a strong promoter results in high R-body expression...machines that can extend and retract over many microns in response to pH changes without the need for ... are composed of 4 proteins encoded in a single operon and were first expressed in E. coli in the 1980s... scaffold that changes shape in response to environmental conditions. More speculatively, R bodies might...of armor to protect their host from predation (Petroni et al 2000). With a better understanding of how...new ways to interact mechanically with their environment.   Jessica Polka is a Postdoctoral Research ... Plasmids from This Publication at Addgene 4. Petroni, G., Spring, S., Schleifer, K.-H., Verni, F. & ...

  5. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...Access to Projection Neurons. Neuron, 92(2), 372–382. https://doi.org/10.1016/j.neuron.2016.09.021 Walters..., 2023). It may be a good choice for targeting neurons, with high transduction frequencies in the central... is derived from AAV2 and engineered to infect neurons and undergo retrograde transport, traveling up ...to target the central nervous system, including neurons, AAV5 is generally the best choice for targeting... year (Dhungel et al., 2025)! It can transduce neurons for retrograde labeling, as well as astrocytes ...enables efficient retrograde targeting of projection neurons and enhances astrocyte-directed transduction. Nature..., S., Bigot, A., Butler-Browne, G., Mouly, V., Negroni, E., & Trollet, C. (2020). Transduction Efficiency...

  6. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ...from a state of chronic hepatitis caused by hypernutrition and alcohol consumption. Chronic inflammation ...cancer and defeat it we need to attack it from all fronts!”  Here I aim to compile a few highlights from ...researchers are preparing and carrying out this multi-pronged attack. Inflammation and tumorigenesis The conference...about alcohol and obesity as driving factors for chronic inflammation which in turn suppresses cancer immunosurveillance...surface receptor PD-1. This immunosuppressive environment in turn may represent a favorable state for tumor...Resources. Studying the immune system and tumor microenvironment Tumor Immunosurveillance and Defense With ...Friedmann-Morvinski about tools used to analyse the tumor microenvironment (one of the plasmids on her poster is pTomo-Ras...

  7. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type
    Blog Post
    ...our observations at the electron microscope showed us that, in monkey neurons, the large majority of the...are powerful tools to modulate the activity of neurons and other brain cells. Since the opsins or chemogenetic... we used AAV viral vectors to express hM4Di in neurons of rhesus monkeys or mice (Galvan et al., 2019)...found a larger proportion on the membrane in mice neurons, most of them were still cytoplasmic. Use of ‘small...hM4Di‐mCherry at the plasma membrane in monkey neurons, we next hypothesized that a different tag fused... of interest the membrane.   Figure 1: Electron microscope images showing ultrastructural localization...protein of interest, an alternative could be bicistronic expression of the fluorescent protein with the...

  8. Important Considerations When Using AAVs

    Type
    Blog Post
    ...oligodendrocytes, microglia, astrocytes, neurons, and inhibitory neurons. Your choice of AAV will depend upon... thanks to our guest blogger Katrina Armstrong. Katrina Armstrong is a Neurophysiology Msc Student at ...post was contributed by guest blogger Katrina Armstrong, a Neurophysiology Msc Student at the University... These AAVs may move toward the cell bodies of neurons (retrograde transport) or towards the synapse (...a larger amount of vector using a serotype with strong CNS tropism (4). If axonal transport is not desirable...neuroanatomical tracing (11). With the ability to produce neuron specific transfection using Cre-lox, this new method... method could allow for labeling of neurons showing a very specific phenotype. Previous neural tracing...

  9. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...plasmids: Luc (intronless; CMV-LUC2CP/ARE and Luc-I (intron-containing; CMV-LUC2CP/intron/ARE). The Luc-I...Luc-I intron is a chimeric β-globin/immunoglobulin intron that has been commonly used in constitutive splicing...Multimerization via the SunTag system also shows strong upregulation of gene activation when fused to dCas9... simplifies the process of creating complex polycistronic lentiviruses. The MuLE toolbox contains a series...induced-pluripotent stem cells (iPSCs) to take on a “neuron-like” phenotype when targeting genes involved in...in mammalian cells, yeast, and Drosophila. Synchronization of secretory protein traffic in mammalian cells...disrupts this interaction and thus triggers a synchronous release of the reporters which can then be tracked...

  10. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...factor RepA binds to the iteron to initiation replication. However, in iteron-containing plasmids,  RepA... regulation system involving antisense RNAs or iterons that inhibits replication when copy number in the...   Figure 1: The replication problem. Iterons are repeated sequences within the origin of replication...del Solar et al., 1998). There are two models for iteron-based plasmid replication control that are not ...the second model, termed the “handcuff” model, iterons of two separate plasmids bind to the same RepA ...these plasmid types? Read our Plasmids 101: Environmental Plasmids blog). Scientists have designed small...replication and transfer systems and host taxonomy. Frontiers in Microbiology 6: . https://doi.org/10.3389/fmicb...

  11. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...tobacco. Studying gene function in C. roseus is strongly limited by the availability of methods. Infection...reporter gene examples are below. The GUS gene (β-glucuronidase) with histochemical GUS-staining is a very useful...days in the dark after the transformation. Use introns Plant promoters can be recognized or leaky in Agrobacterium...Agrobacterium, but not the plant. For example, we have seen strong expression of the GUS gene with the commonly used...Agrobacterium. This can be easily avoided by the use of introns, which will be removed from the transcripts in ...plants, check out this interview with the Nicola Patron, who shared the MoClo plant kit via Addgene. For...well working protocol in your lab, it can become a strong tool for your research. Feel free to add further...

  12. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ... pSC101 copy number is regulated by iterons. RepA binds to iterons and recruits host proteins to the ori...is initiated at the ori and may or may not be synchronized with the replication of the host cell's chromosomal...RepA binds to directly repeated sequences called iterons located near the ori and directly interacts with...check, high copy numbers can allow RepA to link iterons from two different plasmids, forming a pair of ...either of them (Park et al., 2001). The number of iterons encoded on a given plasmid influences how high ...host, as no additional components are necessary. Iterons are not the only tool to stringently regulate plasmid... numbers, RepA proteins form complexes between iterons on different plasmids in a process called "handcuffing...

  13. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...electrochemical sensors for emerging environmental pollutants.” Front Chem. 2014; 2: 41. PubMed PMID: 25019067...variety of applications, including detection of environmental pollutants, drug discovery, and disease diagnosis...instead of protein-based fluorophores, including: Environments with higher temperatures, since proteins can...Jonathan. “RNA Fluorescence with Light-Up Aptamers.” Front Chem. 2016 Jun 28;4:29. PubMed PMID: 27446908. PubMed...systems using immobilized aptamers.” Biosens Bioelectron. 2011 May 15;26(9):3725-36. PubMed PMID: 21419619.... PubMed Central PMCID: PMC4800036. 12. Wang, Rongsheng E., et al. “Aptamer-Based Fluorescent Biosensors...assay for prion diseases diagnostic”. Procedia Environmental Sciences. Volume 12, Part B, 2012, Pages 1348...

  14. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...neural fibers developed by the Anikeeva lab are microelectronic fibers made from thermoplastic polymers that...desired thickness. The fibers are drawn with microelectronics included in them for downstream control – ...trended with expression of the opsin in the target neurons, validating the functionality of these inputs and...Success! They also observed reliable optically evoked neuron activity for at least several months after implantation..., P., & Chandrakasan, A. P. (2020). Modular Optoelectronic System for Wireless, Programmable Neuromodulation... Anikeeva, P. (2019). Flexible fiber-based optoelectronics for neural interfaces. Chemical Society Reviews...A., … Anikeeva, P. (2023). Multifunctional microelectronic fibers enable wireless modulation of gut and...

  15. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...the first light-activated tool for controlling neuronal activity, channelrhodopsin-2 (ChR2), a blue light-activated... exposed to blue light, this channel activates neuronal activity by opening and allowing positively charged...channels (rhodopsins) either excite or inhibit neuronal activity upon exposure to light within a specific...hyperpolarize the cell (Mattis et al., 2012). Bicistronic vectors combine excitation and inhibition by ... when you want to activate or inhibit specific neurons without needing to consider secondary signaling... off-target activity. Whether you are studying neuronal signaling or cancer pathways there are a number...2022). Red Light Optogenetics in Neuroscience. Frontiers in Cellular Neuroscience, 15, 778900. https://...

  16. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ...mutants using error-prone PCR might be a good solution. The process of error-prone PCR is pretty much ...different ways to achieve this goal, including error-prone PCR, degenerate primers, gene shuffling, and structure-guided...BioRender.com.   Generating mutant libraries   Error-prone PCR So, you want to improve an antibody, but don...primers Using degenerate primers is similar to error-prone PCR. Degenerate primers are a group of primers that...instead of forcing sequence diversity using error-prone polymerases, you force sequence diversity using ...using a pool of different primers. While error-prone PCR is usually employed to mutate whole antibody sequences...cytometry for improved binding under defined environmental conditions. These methods are especially powerful...

  17. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...might think that being able to figure out what went wrong with a PCR is a pretty niche skill; outside of a...about seven different skills to you, you are not wrong. Problem-solving overlaps heavily with other skills...core part of our academic training.  Developing strong problem solving skills is just a matter of applying...As such, scientists are uniquely prepared to be strong problem solvers both in and out of the lab. The...position. Of course, there are limits to how far strong problem solving skills alone can take you - just...effectively does not mean I am qualified to be an astronaut. But, knowing that this skillset is a part of ... Ashley Waldron...

  18. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ... How do you visualize protein turnover within a neuron? For my PhD I studied a synaptic protein that is... to study how protein turnover is regulated in neurons. Protein turnover is the combined effect of protein... I wanted to measure it’s levels in its normal neuronal context. In other words, I needed a method for...for visualizing protein turnover in situ in neurons. The classic approach for studying protein turnover... whole population of one particular protein in neurons or in any other cell type. However, we cannot distinguish... newly synthesized proteins in rat hippocampal neurons." Nature neuroscience 13.7 (2010): 897-905. PubMed...Robert H. Singer. "Single β-actin mRNA detection in neurons reveals a mechanism for regulating its translatability...

  19. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ... photons are absorbed by matter, they promote electrons within atoms to a higher energy state. This process... called excitation. After a short period, the electrons revert to a lower energy state, whereby a photon...those of absorbed photons. When photons excite electrons of a fluorophore, they can do so at a range of...by the cytometer. Brighter fluorophores have a stronger signal. Table 1: Degrees of brightness for a few...absorbed and then emitted, which creates a relatively stronger — or brighter — signal.   Figure 2: Excitation... real-life advantage of a brighter fluorophore? Strong brightness makes it easier to distinguish a signal...and FITC an organic molecule), their bleed is so strong that these spectra almost overlap. Hence, they ...

  20. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    ...be used for sparse labeling of neurons for tracing experiments.  Neuronal tracing experiments: While synthetic...broad number of cell types, such as inhibitory neurons, rods, cones, or retinal ganglion. Rather than ...that produce sparse labeling would be useful for neuronal tracing experiments where lower levels of labeling...labeling makes it easier to trace a neuron’s projections. Generating animal models: Transgenic animals allow...for retinal gene therapy Find more about precise neuronal targeting using AAV technologies Browse all of... H.P., Krol, J., & Roska, B. (2019). Targeting neuronal and glial cell types with synthetic promoter AAVs...
Showing: 141 - 160 of 682 results