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We narrowed to 450 results for: abo.5

Showing: 141 - 160 of 450 results

  1. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...similarly but not identically to BE3, modifying a 3-5 base window 18 bases upstream of the PAM.  David Liu...than BE2 Nishida et al., 2016 Target-AID   Edits 3-5 base window surrounding -18 position upstream of the...CRISPR-based cytosine base editing. Nature Biotechnology, 41(5), 663–672. https://doi.org/10.1038/s41587-022-01532...generated from TadA variants. Nature Biotechnology, 41(5), 686–697. https://doi.org/10.1038/s41587-022-01611...low off-target activity. Nature Biotechnology, 41(5), 673–685. https://doi.org/10.1038/s41587-022-01533...Base Transversion Editors Learn about base editing for plants Read about four base editor reporters Additional... The BE3 system increased editing frequency to above 30% for a variety of targets in human cell lines...

  2. Retrograde AAV viral preps

    Type
    Collection
    ...DREADD Gether 83896 pAAV-hDlx-GiDREADD-dTomato-Fishell-5 hDlx Inhibitor DREADD Fishell 83897 pAAV-hDlx-GqDREADD-dTomato-Fishell...serotype from the Alla Karpova and David Schaffer laboratories. These viral vector preparations were produced...Janelia Research Campus viral project team in collaboration with David Schaffer (University of California...

  3. Hot Plasmids Podcast Episode 2: New RFPs, AAVs, & More

    Type
    Blog Post
    ...Advice on Picking a Graduate Lab and Project Check Out 5 Science Videos from Tom McFadden Resources at Addgene.org...of our Hot Plasmids podcast series, you'll learn about new red fluorescent proteins, AAV tools for targeting...

  5. The time and cost required to make a plasmid

    Type
    Blog Post
    ...Addgene Blog 5 factors to help you choose the right cloning method Find blog posts about scientific sharing...scientists save even more!) If you assume an average of 5 hours of hands-on time per plasmid, Addgene, since...cloning before you can start your experiment? What about all the reagents you need to order? Sometimes, it...dive deep into generating the data you’re excited about. When we were closing in on the one million plasmids...million plasmids shared than not!), we think it’s about time we updated this post. How much time does ...

  6. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...LTRs define the limit of what will be packaged. The 5’ LTR and 3’ LTR, respectively, act as RNA polymerase...adenovirus types, but most vectors are based on adenovirus 5 (Ad5).     Fig. 3 - Viral vector components...aren’t reflected in the common components outlined above for gamma-retroviruses. While not required for lentiviral...and helper plasmids for all the viruses discussed above (gamma-retrovirus, lentivirus, adenovirus, and adeno-associated.... Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press; 1997. Principles of Retroviral Vector ...

  7. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ... Antibody Validation proposed 5 pillars for antibody validation.  The 5 pillars include genetic strategies...Narrowing the field After working your way through the above steps, you may find that you still have a number...advice if you need to troubleshoot. What do you know about the vendor and the vendor’s technical support? An...validation is equal and you should think critically about what data is and is not shown on an antibody website...

  8. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...set at .05, then 5% of the time the statistical test will be incorrect. If you have 5 samples and perform...comparison is needed, without a high number of samples (~5). Tukey’s test – Most conservative test. Higher false...a summary snapshot without telling you anything about outliers or data spread within those data sets. ...the rules” and which we have certain information about. The assumptions and requirements that need to be...Quantitative If your data doesn’t fit the requirements above, a non-parametric test may be the way to go, which...only performing the comparisons you really care about. Family-wise error is the probability of a false...rate than the multiple comparison tests described above. Violated assumptions and statistics Statistical...

  9. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...transcription systems in Drosophila.” Nat Neurosci 9(5) (2006): 703-9. PubMed PMID: 16582903. Potter, Christopher...Caenorhabditis elegans.” Nat Methods 9(4) (2012): 391-5. PubMed PMID: 22406855. PubMed Central PMCID: PMC3846601...promoters.” Proc Natl Acad Sci U S A. 80(1) (1983): 21-5. PubMed PMID: 6337371. PubMed Central PMCID: PMC393301...can be used in the repressible manner described above, as well as the inducible manner detailed in our...necessary to have all 3 of the different binary systems above if they all behave very similarly?” The simple answer... at the same time.  The 3 binary systems listed above are orthogonal, meaning that they do not affect ...

  10. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...part A of the transgene contains a promoter and the 5’-fragment of the transgene, followed by a splice site...part A of the transgene contains the promoter, the 5’ half of the transgene, a splice donor sequence and... degron, or degradation sequence, upstream of the 5’ splice donor and downstream of the 3’ splice acceptor...first time AAV user Learn about how AAV is used in retinal gene therapy Learn about the parts of an AAV transfer...

  11. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...sequence and has been suggested to cause cytotoxicity [5]. Second, instead of using a donor-plasmid, you could...PMID: 23723351. PubMed Central PMCID: PMC3690869. 5. D. Grégoire and M. Kmita, “Recombination between ...retroviral insertion sites.,” Nat. Protoc., vol. 4, no. 5, pp. 789–98, Jan. 2009. PubMed PMID: 19528954. PubMed...transposase itself, please read on to learn more about this exciting technology from the folks at Transposagen...Additional Resources on the Addgene Blog Learn about the Sleeping Beauty Transposase Read up on Genome...

  12. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...modifications, specifically ones which do not contain a 5’ triphosphate in the gRNA, reduce the innate immune...Controlled CRSIPR-Cas-based Genomic Regulation. ACS 6, 5, 695-703 (2020). https://pubs.acs.org/doi/10.1021/...“I hope it isn’t degraded” when scientists talk about their precious RNA samples. RNA is inherently less...efficacy as well as the unnecessary stress of worrying about the status of your RNA. gRNAs modifications are ...Some of the RNA stabilizing modifications listed above were developed before CRISPR-Cas9, for use in applications...

  13. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    .... 1993 Dec 5;234(3):534-41. PubMed PMID: 8254658. 5. Bernard, P. and Couturier, M. “Cell killing by the...ccd operon in plasmid F.” J Bacteriol. 1989 May;171(5):2353-60. PubMed PMID: 2651399. PubMed Central PMCID...in modern cloning, and why should you learn more about it? Read on to find out how ccdB can make your cloning...of this system for enhancing cloning efficiency about 20 years ago and developed cloning vectors to harness...

  14. Enabling high school research at the Journal of Emerging Investigators

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    Blog Post
    ...assess whether or not their achieving it. Chapter 5 - JEI's future - getting more involved in the classroom...working in a lab for the first time, and thinking about the future. Many thanks to our guests,Olivia Ho-Shing...resources Use our Plasmids 101 eBook to teach students about basic plasmid biology Use or CRISPR 101 eBook to...

  15. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...expertise. 5. Present your work often Surprisingly, many PFs are reluctant to give talks or posters about their...position would begin- for you, at the beginning of Year 5. Now work backwards. To publish a submitted manuscript...have finite time and resources. You typically have 4-5 years to make discoveries and publish papers to position...Nature and Science, for example). This blog post is about how to get the most out of your postdoctoral training...your new model?  PFs need to be regularly thinking about manuscripts. The other items will follow. If you...this is a strong indicator that this person cares about your science and your development. Taken together...Researchers face a fundamental conflict. Science is about exploration, discovering new information. There ...

  16. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ... tracked at 5 minute intervals via Western blot. Phosphorylation of MEK1/2 peaks at just 5 minutes post-Raf1...Additional Resources on the Addgene Blog Learn More about Photoactivatable Fluorescent Proteins Control Protein...

  17. New Videos: Addgene Lab Tips

    Type
    Blog Post
    ...New Educational Resource: The Addgene Videos Page 5 Science Rap Videos and Their Creator: Tom McFadden...locate materials quickly and easily. Learn more about using barcodes. Check out our “Lab Tip” filter...

  18. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...epitope. Epitopes are between 5-8 amino acids long, with a typical length of 5 or 6 amino acids (Cruse et...facilitated by an enzymatic reaction. Figure 5: Immunofluorescence staining for CD31 (green), CARD14...is referred to as an antigen. You can learn more about antibodies in our Antibodies 101 animation, What... expressing this protein. Read on to learn more about antibodies and how to use them in the lab! Parts...lots is a big concern. You can find more details about polyclonal antibodies on our Antibodies 101: Polyclonal...not necessarily what you are getting. Learn more about monoclonal antibodies here. Recombinant antibodies... as well as compare between samples. Learn more about flow cytometry. Cell and tissue labeling Antibodies...

  19. Important Considerations When Using AAVs

    Type
    Blog Post
    ...etc.) (4). This has also been identified with AAV2 (5), AAV6 (6) and AAV5 at excitatory connections (3)....tropism and axonal transport of AAV serotypes 1, 2, 5, 6, 8 and 9 in the mouse brain." PloS one 8.9 (2013...PMID: 24694006. PubMed Central PMCID: PMC4137353. 5. Ciesielska, Agnieszka, et al. "Anterograde axonal... Its Fruit Is Sweet The Future? I knew little about adeno-associated Viral Vectors (AAVs) before starting...I’ll provide some tips that will help you think about how to get started with AAVs. Since my research ...understanding of receptor physiology and the effects of metabotropic and ionotropic influences on overall cell function... the biotechnology firm Biogen have recently collaborated to develop “AAV 3.0”, vectors that will have...

  20. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...in Sweden. It can be very time consuming to design 5 guide RNAs (gRNAs) targeting each of the 1000 genes...constellations: Doench/Root (2), Zhang (3), Wu (4), Yusa (5), Wang/Lander/Sabatini (6), and Chari/Mali/Church ...PMID: 26190106. PubMed Central PMCID: PMC4559080. 5. Tzelepis, Konstantinos, et al. "A CRISPR dropout ... to design gRNAs for all the purposes discussed above. Please see videos and text on the website for more...Output: A text file containing full information about the suggested gRNAs to use, including all information... hard to read. It´s suggested that you open the above text files, copy the content of the file, and paste...Additional Resources on the Addgene Blog Learn about CRISPR Screens How to Design Your gRNA Tips for ...
Showing: 141 - 160 of 450 results