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We narrowed to 168 results for: crispr library

Showing: 141 - 160 of 168 results

  1. CRISPR Plasmids - Yeast

    Type
    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... CRISPR Yeast CRISPR Plasmids: Yeast Browse ... following CRISPR plasmids have been designed for use in yeast. Cut Fully functional CRISPR/Cas enzymes...CRISPR plasmids that have been designed for use in yeast. CRISPR...Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian... Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand...

  2. CRISPR 101: Cytosine and Adenine Base Editors

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    Blog Post
    ...Addgene Blog CRISPR 101: Homology-Directed Repair CRISPR 101: Cytosine Transversion Editors CRISPR 101: Any...Addgene.org Catch up on your CRISPR background with our CRISPR Guide Browse CRISPR plasmids for base editing...Early CRISPR applications were often limited by the low editing efficiency of homology-directed repair... indels were observed than point modifications. CRISPR base editing persists through multiple cell divisions...editing based on Cas9 off-target activity.  Find CRISPR base editing plasmids at Addgene Improving cytosine...near-PAMless Cas9 variants significantly expands the library of accessible genomic targets for base editors ...which you can learn more about in this blog post: CRISPR 101: RNA Editing with Cas13. Finally, the development...

  3. Neurodegeneration Research Collection

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    Collection
    ... Elife. 2023 Oct 24. See More CRISPR Tools Find CRISPR pooled libraries available from Addgene, sortable...guide to using plasmid pooled libraries . New and Noteworthy: Use a CRISPRi system to target alpha-synuclein...neurodegeneration research. Disease Info Plasmid Collection CRISPR Tools AAV Viral Preps iPSC Differentiation Factors...about the blood brain barrier integrity, and using CRISPR screens to understand more globally how neurons.... Target neural oxytocin receptors using an AAV-CRISPR/Cas9 strategy for gene editing across divergent...

  4. CRISPR History and Development for Genome Engineering

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    Collection
    ...like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR revolution shows no...information on CRISPR, check out Addgene’s CRISPR Guide and CRISPR blog posts , or find CRISPR plasmids for... CRISPR CRISPR History CRISPR History and Development for Genome Engineering You may also like...sequencing, CRISPR is a robust system for genome-wide screening. CRISPR Technological Advances CRISPR technology...finger nucleases (ZFNs) and TALENs. CRISPR: An Adaptive Immune System CRISPR (Clustered Regularly Interspaced...prokaryotes used CRISPR as part of an adaptive immune system - utilizing various CRISPR-associated (Cas...of DNA known as CRISPR arrays. Separate Cas proteins then express and process the CRISPR loci to generate...

  6. CRISPR Plasmids - Prime Edit

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    Collection
    ... CRISPR Prime Edit CRISPR Plasmids: Prime Edit Browse CRISPR Plasmids By Function ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Prime...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian... . Browse, sort, or search the tables below for CRISPR prime editing plasmids. Plasmids are available ...

  7. CRISPR Plasmids - Bacteria

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    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Base... CRISPR Bacteria CRISPR Plasmids: Bacteria...following CRISPR plasmids have been designed for use in bacteria. Cut Fully functional CRISPR/Cas enzymes...CRISPR plasmids that have been designed for use in bacteria. CRISPR...Plasmid Gene/Insert Promoter PI Publication Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...mutations. ID Plasmid Promoter PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand...

  8. CRISPR Plasmids - Mammalian Expression

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    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Base...CRISPR plasmids that have been designed for use in mammalian systems. CRISPR... CRISPR Mammalian Expression CRISPR...CRISPR Plasmids: Mammalian Expression The following CRISPR plasmids have been designed for use in mammalian...mammalian expression systems. Cut Fully functional CRISPR/Cas enzymes will introduce a double-strand break (...Promoter Selectable Marker PI Publication Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...

  9. Depositor Collections

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    Collection
    ...Browse depositor collections of antibody, CRISPR, disease research, visualization, protein expression...Plasmid Kits Plasmid Special Collections Pooled Libraries Antibodies NeuroMab/Trimmer Lab Recombinant mAb...

  10. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...latest work, the team designed and screened a large library of iGluSnFR3 mutants, identifying the two  best-performing...editing, think beyond Cas9 with STITCHR: a combined CRISPR / retrotransposon system with the pithy name "site-specific...site-specific target-primed insertion through targeted CRISPR homing of retroelements." The Abudayyeh-Gootenberg...

  11. CRISPR Plasmids - Plants

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    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... CRISPR Plants CRISPR Plasmids: ...following CRISPR plasmids have been designed for use in plants. Cut Fully functional CRISPR/Cas enzymes...CRISPR expression plasmids that have been designed for use in plants. CRISPR...: Plants Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian... Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand...

  12. Bacterial Expression Systems

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    Collection
    ...with Addgene's CRISPR Guide . Browse CRISPR plasmids available at Addgene through our CRISPR Plasmids and...tools like CRISPR-Cas9 are not only valuable but often essential. Find a collection of CRISPR plasmids ...Bacterial CRISPR Plasmids page . Additional Addgene Genome Engineering Resources Learn more about CRISPR with...zinc finger proteins. Addgene Blog CRISPR Methods for Bacteria CRISPR-based Genome Editing Systems For ...Empty Backbones Collection Molecular Biology Guide CRISPR Guide Bacteria are commonly used to create, store...MoClo Toolkit (Addgene #1000000080) features a library of promoters and purification tags compatible with...Biosensors Kit (Addgene #1000000021) is a vector library for the generation of FRET biosensors for ratiometric...

  13. Fluorescent Proteins 101: Aptamer Fluorophores

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    Blog Post
    ...sorting (FACS) of E. coli transformed with aptamer libraries, the Jaffrey group selected for a 49-nucleotide... References 1. Ma, Hanhui, et al. “Multicolor CRISPR labeling of chromosomal loci in human cells.” Proc...

  14. CRISPR Plasmids - Tagging

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... CRISPR Protein Tagging CRISPR Plasmids...Read our CRISPR 101 post for more information about HDR/repair template design. How to use CRISPR to tag...for a CRISPR-based system to add a tag (currently using FLAG) to endogenous proteins. This CRISPR/Cas plasmid-system...SapTrap CRISPR/Cas Toolkit SapTrap is a modular toolkit from Erik Jorgensen's lab for building CRISPR targeting...CRISPR plasmids for endogenous tagging of your gene of interest. CRISPR...Plasmids - Protein Tagging Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit...

  15. Guide to Using Pooled Libraries

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    Guide
    ...most popular libraries! Genome-wide Screening Using CRISPR/Cas9 Lentiviral CRISPR Libraries Enable Genome-Scale...Amplifying and Using the Library Once you receive your library from Addgene, check the library information to see...this creates a pooled lentiviral CRISPR library. Apply pooled library to cells. DIVERGE: Positive and ...Overview of what pooled libraries are; how to amplify and use a pooled library; and what types of screens... Science Guides Guide to Plasmid Pooled Libraries Plasmid Pooled Libraries Jump To: ...: Pooled Library Guide Introduction Library Amplification Types of Screens Resources An Introduction to... to Pooled Libraries Pooled libraries represent a powerful tool for forward genetic screening, or identifying...

  16. Cancer and the Immune System: Deciphering the Relationship

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    Blog Post
    ... first ever CRISPR-based clinical trial in 18 lung cancer patients. This study used CRISPR gene editing...Our Cancer Collection Pages Use the Clon Tracer Library to Research Cancer Drug Resistance Checkout the...

  17. CRISPR Guide

    Type
    Guide
    ...screening using CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries...gene, so CRISPR libraries contain thousands of unique gRNAs. gRNA design for CRISPR libraries is usually...post on lentiviral pooled libraries . Browse Libraries: CRISPR Pooled Libraries Cas9 Fusion Tools dCas9 cannot...Guide CRISPR Guide Jump To: CRISPR Guide Overview CRISPR Basics Small Edits Large Edits Genome-Wide Screening...Figure 7: Overview of CRISPR transposases Browse Plasmids: CRISPR transposases CRISPR Recombinases and Integrases...genome-wide screens using CRISPR involves pooled lentiviral CRISPR libraries. Figure 8: Overview of genome-wide...Non-mammalian CRISPR libraries are also available, such as those for yeast or drosophila . Although CRISPR has ...

  18. Science Guides

    Type
    Guide
    ... below. Antibodies Chemogenetics CRISPR Optogenetics Pooled Libraries Viral Vectors Antibodies Produced...Read More CRISPR Class 2 C lustered R egularly I nterspaced S hort P alindromic R epeat (CRISPR) systems...background information on CRISPR and the basics for planning your first CRISPR experiment. Read More Optogenetics...understand their functions. Read More Pooled Libraries Pooled libraries are single preparations of many different...given library have the same backbone, but they express or target different genes. Some libraries cover...

  19. Lentivirus Plasmids

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    Collection
    ...lentiviral plasmids to express your CRISPR gRNA? Please see our CRISPR Empty gRNA plasmids table and search...Titering Your Lentiviral Preps Lentiviral Pooled Libraries All Viral Plasmids Packaging Plasmids Transfer...

  20. Modular Cloning Guide

    Type
    Guide
    ...multiplexed CRISPR editing, and more. Fungal Toolkit for Modular Cloning (FTK) Fungal Expression, CRISPR Arnold...experiments. Multiplex CRISPR/Cas9 Assembly Systems Genome Engineering, CRISPR, Mammalian Expression Takashi...MoClo-compatible CRISPR/Cas9 cloning kit with intronized Cas9 Genome Engineering, CRISPR Sylvestre Marillonnet...SpCas9-coding gene. MoClo CRISPR/Cas Toolkit for Plants Genome Engineering, CRISPR, Plant Expression Vladimir...Engineering, CRISPR, Plant Expression Johannes Stuttmann Plasmids for assembly of constructs for CRISPR genome...Genome Engineering, CRISPR, Yeast Expression Brigitte Gasser Plasmids to perform CRISPR/Cas9 mediated genome...(a.k.a. K. phaffii ). NT-CRISPR Plasmid Collection Genome Engineering, CRISPR, Bacterial Expression Anke...
Showing: 141 - 160 of 168 results