We narrowed to 413 results for: his

1. From Lab Bench to Leadership: Transitioning into Management as a PhD

   Type

   Blog Post

   ..." He successfully transitioned to industry after his PhD and a couple of postdocs focusing on molecular...

2. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

   Type

   Blog Post

   ...Division of Life Sciences at Rutgers University. His dissertation and research background is in citizen...

3. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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   ...team used nickel-coated agarose beads coated with his-tagged OptoNBs. OptoNB coated beads were then imaged...

4. 3 Challenges in Plant Synthetic Biology

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   ... can be found climbing mountains or playing with his two cats. Keira Havens grew up in Hawaii where she...

5. Kiran Musunuru on the Newest TALEN Genome-Editing System

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   ...the kit now makes possible, and how it compares to his CRISPR/Cas9 system. Addgene: For people unfamiliar...modeling in stem cells. Addgene: Tell me about the history. How had investigators done this kind of work before...

6. Troubleshooting Your Plasmid Cloning Experiment

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   ...cloning of more than 10,000 DNA custom constructs. His work was acknowledged in hundreds of publications...

7. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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   ...as well as cell lineage studies. Eisenhoffer and his colleagues evaluated CreLite for cell tracing using...

8. Hot Plasmids - May 2022

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   Blog Post

   ...allow researchers to avoid the off-target effects of hi-multiplexing within in a cell, while potentially ...

9. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

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   Blog Post

   ...our guest blogger Jon Chow. Jon Chow is finishing his immunology PhD at Harvard University. He has had ...

10. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

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    ... At the Broad, my colleague Cory Johannessen and his team have recently demonstrated the power of this...one assay per gene approach that has been the historical standard. Specifically, in our recent publications...

11. The time and cost required to make a plasmid

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    Blog Post

    ...traditional “one at a time” approach. According to his math, it takes five hours of hands-on time to make...

12. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

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    Blog Post

    ..., courtesy of Addgene depositor Stephen Elledge. His new method, named sequence- and ligation-independent...

13. Sharing is Caring: But How to Distribute Open Hardware?

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    ...   About the author: Benedict Diederich received his PhD from the Heintzmann Lab at the Leibniz IPHT Jena...

14. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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    Blog Post

    ...Alabama in Huntsville explains it, a major goal in his laboratory is to understand the function of the non-coding...

15. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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    ...start site in those 15,977 human genes. Weissman and his colleagues demonstrated in a paper in Cell that they...

16. RANbodies: Reporter Nanobody Fusions

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    ...them easy to harvest from cell culture media. Their His tags allow for further purification, if desired, ...targeted the following antigens: GFP, RFP,  the histone protein H2A2B, and the actin-binding protein gelsolin...however, compatible with most multi-labeling immunohistochemical protocols where either a directly labeled...RANbodies) as versatile, small, sensitive immunohistochemical reagents. PNAS. PubMed PMID: 29440485. Additional...

17. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

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    Blog Post

    ...Allinen et al., 2004). In fact, Sanford H. Barsky and his team tested the influence myoepithelial cells had...breast tumorigenesis: Friend or foe?." Histology and histopathology (2018): 18021-18021. PubMed PMID: 29978449...

18. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

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    Blog Post

    ...sensitive GFP2 (roGFP2)-based biosensors for live cells. His work focuses on the enzyme hydropersulfide-propagating... light activation and CNO silencing. (D) Immunohistochemistry verification of the dual virus expression...

19. Human Germline Editing Using CRISPR

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    Blog Post

    ...paper to understand what Shoukhrat Mitalipov and his colleagues have achieved, and how the field will ...

20. Addgene: The First Twenty Years

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    Blog Post

    ...Béthune, Professor at HAW Hamburg, posting about his Blue Flame Award on Bluesky. In 2016, we also began...