...team used nickel-coated agarose beads coated with his-tagged OptoNBs. OptoNB coated beads were then imaged...

...the Ragon Institute of MGH, MIT, and Harvard, and his lab found that the neutralization effectiveness of...

...bacteria interact with each other and plant surfaces. His system of choice is the Arabidopsis thaliana - Pantoea...serotonin receptor to study how single nucleotide polymorphisms present in the human population can affect drug...

...our guest blogger Jon Chow. Jon Chow is finishing his immunology PhD at Harvard University. He has had ...

...functions as one of the “healthy human volunteers” in his studies! This was followed by a talk from Professor...Immunosurveillance and Defense With increasingly sophisticated visualization techniques it is possible to follow...

... can be found climbing mountains or playing with his two cats. Keira Havens grew up in Hawaii where she...

...the kit now makes possible, and how it compares to his CRISPR/Cas9 system. Addgene: For people unfamiliar...modeling in stem cells. Addgene: Tell me about the history. How had investigators done this kind of work before...

...allow researchers to avoid the off-target effects of hi-multiplexing within in a cell, while potentially ...

..., courtesy of Addgene depositor Stephen Elledge. His new method, named sequence- and ligation-independent...

...traditional “one at a time” approach. According to his math, it takes five hours of hands-on time to make...

...   About the author: Benedict Diederich received his PhD from the Heintzmann Lab at the Leibniz IPHT Jena...

...as well as cell lineage studies. Eisenhoffer and his colleagues evaluated CreLite for cell tracing using...

...cloning of more than 10,000 DNA custom constructs. His work was acknowledged in hundreds of publications...

...our guest blogger Jon Chow. Jon Chow is finishing his immunology PhD at Harvard University. He has had ...

... At the Broad, my colleague Cory Johannessen and his team have recently demonstrated the power of this...one assay per gene approach that has been the historical standard. Specifically, in our recent publications...

...Alabama in Huntsville explains it, a major goal in his laboratory is to understand the function of the non-coding...

...start site in those 15,977 human genes. Weissman and his colleagues demonstrated in a paper in Cell that they...

...them easy to harvest from cell culture media. Their His tags allow for further purification, if desired, ...targeted the following antigens: GFP, RFP,  the histone protein H2A2B, and the actin-binding protein gelsolin...however, compatible with most multi-labeling immunohistochemical protocols where either a directly labeled...RANbodies) as versatile, small, sensitive immunohistochemical reagents. PNAS. PubMed PMID: 29440485. Additional...

...sensitive GFP2 (roGFP2)-based biosensors for live cells. His work focuses on the enzyme hydropersulfide-propagating... light activation and CNO silencing. (D) Immunohistochemistry verification of the dual virus expression...

...Béthune, Professor at HAW Hamburg, posting about his Blue Flame Award on Bluesky. In 2016, we also began...