We narrowed to 532 results for: lias

...your training? These could serve two purposes – familiarize you with the techniques you will be learning ...you get started!) Are there protocols you can familiarize yourself with? If your bench mentor hasn’t sent...the protocol we’ll be using next week so I can familiarize myself with it.” It won’t be a bother to them...

...proper controls are invariably inconclusive and unreliable. Proper controls provide the constant variables...backbone as Plasmid A—that does not target any mammalian gene. This non-targeting control is critical to.... Replicate controls: technical and biological Reliable results are reproducible from properly controlled...

... nuclease domains: pST1374 (for expression in mammalian cells or zebrafish) pDW1775 (for expression in... domain. Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish (can be used as a substitute...domain ( Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish). pMLM802 (for expression of...domain ( Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish). pMG10 (a '“2-in-1” ZFN cassette...domain ( Sollu et al., Nucleic Acids Res ) in mammalian cells or zebrafish). pCLR2068 (for expression ...domain. ( Doyon et al., Nat Methods 2011 ) in mammalian cells or zebrafish as described by Moore et al...

...expressing proteins in bacteria, yeast, insect and mammalian cells. Check out Kay et al., 2009 for methods ...purification of tagged transcription factors in mammalian cells and transgenic mice.” PNAS 100.13 (2003)..., M., & Ting, A. Y. “Imaging proteins in live mammalian cells with biotin ligase and monovalent streptavidin...

...have been using AAV vectors for gene editing in mammalian cells since 1998. In that year, Russell and Hirata...zygotes and thereby correct a mutation that causes Hemophilia B. Unfortunately, work combining CRISPR with ...postnatal administration of AAV vector cures haemophilia B mice." Scientific Reports 7.1 (2017): 4159....

...bloggers Dominik Paquet and Dylan Kwart from Ludwig-Maximilians-University in Munich and Marc Tessier-Lavigne...to be incorporated into the edited genome. In mammalian cells such as stem cells, HDR is relatively rare...Institute for Stroke and Dementia Research, Ludwig-Maximilians-University Munich. His lab develops iPSC-based...

...care about. Don’t try to present topics that are unfamiliar to you because the company you are visiting works...unless you are certain that the audience will be familiar with the terms. If you feel you need to define...lecture.This takes practice (see above) and a deep familiarity with your content. If at all possible, practice...

... There are a couple different approaches to be familiar with when talking about IF, direct and indirect...antibody that you consider in order to determine how reliable the antibody seems and what sort of protocol you..., will help make antibodies more effective and reliable in the future.  We hope that this post has shed...

...Destination constructs were designed for optimal mammalian expression of Voytas Golden Gate-assembled TALEN...destination vectors and a reporter vector for mammalian cell-based validation assay that are designed ...vectors, pcDNA-TAL-NC2 and pCAGGS-TAL-NC2, are mammalian expression/mRNA synthesis vectors containing unique...Torres-Padilla These plasmids were designed for mammalian expression of fluorescent TAL effectors to visualize...plasmids were optimized for TALEN expression in mammalian systems including hard-to-transfect cell lines...sequence to drive efficient TALEN expression in mammalian cells, (iv) an improved, truncated TALE backbone...and pTAL7b for enrichment of double-transfected mammalian cells. In addition, a T7 promoter was included...

...subdivisa (CsChR), Chloromonas oogama (CoChR), and Scherffelia dubia (SdChR). ShChR, nicknamed Chronos, is blue...catalytically inactive Cas9 DNA-binding domain in mammalian cells. (Guilinger et al., Nat Biotechnol. 2014... in the mouse brain. Kim et al is utilizing a mammalian GRASP (GFP reconstitution across synaptic partners...and have been tested in bacterial, insect and mammalian cells. These vectors were designed for parallel...

...45), there are also initiatives to create more reliable reagents for future research. I’ve talked to many...many researchers who have complaints about the unreliability of antibodies. IPI is working to address this...repeat previously published results. This over-reliance on the perfect story for a “high impact” publication...

...with, making them the go-to choice for studying mammalian biology. Similar to any model, mice are not without...https://doi.org/10.1016/j.cell.2013.04.025 Zhang X, Liang P, Ding C, Zhang Z, Zhou J, Xie X, Huang R, Sun ...srep32565 Resources on the Addgene Blog CRISPR 101: Mammalian Expression Systems and Delivery Methods Learn ...

... developed by the GENIE Project team at HHMI's Janelia Research Campus (Aggarwal et al., 2025).In this...CMV promoter drives robust gene expression in mammalian cells. Flexible tagging: Choose untagged or add...expressing tagged proteins, and stay tuned for mammalian expression and AAV plasmid sets launching later...

...targeting astroglia. It has additionally been reported to have tropism for liver, vascular endothelial, and...Vectors Mediate Efficient Transduction of Airway Epithelial Cells in Mouse Lungs Compared to That of AAV2...

...Return to top Plasmids Encoding Mammalian Genes or Inserts Several mammalian genes have been identified as...SARS-CoV-2 Plasmids Other Coronavirus Plasmids Mammalian Targets General Plasmid Tools Pooled Libraries... SARS-CoV-2 plasmids for E. coli , yeast, and mammalian expression, and spike protein...cell protein 4) is involved in beating of airway cilia (Kogiso et al., 2020) Cytokines - SARS-CoV-2 infection...CRISPR tools, like Cas12a and Cas13a plasmids, to reliably detect nucleic acids SARS-CoV-2 main protease ...., Marunaka, Y., & Nakahari, T. (2020). Airway Ciliary Beating Affected by the Pcp4 Dose-Dependent [Ca2...window) Wang, K., Chen, W., Zhang, Z., Deng, Y., Lian, J. Q., Du, P., Wei, D., Zhang, Y., Sun, X. X., ...

...8 CKLFSF8, CKLFSF8-V2 CNTF ciliary neurotrophic factor HCNTF CNTFR ciliary neurotrophic factor receptor...glucagon-like peptide 2 receptor - GMFB glia maturation factor, beta GMF GMFG glia maturation factor, gamma GMF-...vascular endothelial growth factor A MGC70609, MVCD1, VEGF, VEGF-A, VPF VEGFB vascular endothelial growth...Categories Plasmid Tables Additional Resources Mammalian organisms are exposed to millions of potential...histocompatibility complex, class II, DQ alpha 1 CD, CELIAC1, DQ-A1, FLJ27088, FLJ27328, GSE, HLA-DQA, MGC149527...histocompatibility complex, class II, DQ beta 1 CELIAC1, HLA-DQB, IDDM1 HLA-DRA major histocompatibility...HsT17083, P58, PI-PLC PROCR protein C receptor, endothelial (EPCR) CCCA, CCD41, CD201, EPCR, MGC23024, bA42O4.2...

...is variable transduction of oligodendrocytes, microglia, astrocytes, neurons, and inhibitory neurons. ...Serotype Oligodendrocytes AAV8 > AAV2 & AAV6 Microglia AAV9 > AAV2 & AAV6 Astrocytes AAV8 > AAV2 & ...Anterograde axonal transport of AAV2-GDNF in rat basal ganglia." Molecular Therapy 19.5 (2011): 922-927. PubMed...

...mentioned above and is a popular RNAi trigger in the mammalian research community. Fortunately, many tools for...such as in the model flowering plant Arabidopsis thaliana (Li et al., 2013; Zhang et al., 2018) and the ...posts Resources on Addgene.org Find tools for mammalian RNAi Browse all plasmids for RNAi Check out Addgene's...

...engineered to eliminate binding of endogenous mammalian transcription factors to further minimize background... expression of the transactivator proteins in mammalian cells or other species by codon optimization of...expression of EGFP Tet-On 3G rtTA TRE3GS Xiaojun Lian 171123 pLVX-TetOne-Puro-GFP Lentiviral Tet-On vector...Tet-On 3G rtTA Oskar Laur 96963 pCAG-TetON-3G Mammalian expression of the Tet-On 3G transactivator Tet-On...plasmids. tTA Viviana Gradinaru 104102 pCAG-tTA Mammalian expression of tTA from the CAG promoter for Tet-Off... 172878 pSF3-ultraID Expression of UltraID in mammalian cells with the Tet-On or Tet-Off system Julien...Joan Brugge Return to Top Additional Resources Mammalian shRNA Plasmids Cre-lox Plasmids Stem Cell Research...

..., the Qi lab employed a gRNA-based design more familiar to those using CRISPR in eukaryotes (Qi et al....transcription of one or more genes in E. coli. and in a mammalian cell line (Qi et al., 2013). Two important features...two tandem copies of the gRNA cassette, one can reliably knockdown expression of multiple target transcripts...