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...Addgene Viral Vectors 101 eBook Viral Vectors 101 Youtube Videos Addgene's Biosafety Resources Resources ...

...Reagents Opti-Prep (60% Iodixanol) QuickSeal tubes QuickSeal tube spacers 16 ga needle 18 ga needle 10 mL ... formulation buffer) to top off the tube. Seal the QuickSeal tubes. Centrifuge at 350,000 x g for 90 min... QuickSeal tube with a 16 ga needle and start collecting 0.5 mL to 1 mL fractions per tube. Avoid the ...mL fractions in microcentrifuge tubes. Repeat for each QuickSeal tube. The first 3 mL collected corresponds...phenol red Underlay each solution into a QuickSeal tube in the order below using a 10 mL syringe and a blunt...,000 x g at 18 °C. Carefully take the QuickSeal tubes out of the rotor and place them in a stable rack...a row of roughly 20 open 1.5 mL microcentrifuge tubes in a rack. These will be used to collect fractions...

...cannot fit into a single centrifuge tube, aliquot it into several tubes/bottles. Remove the supernatant and...stock culture to a 1.75 mL microfuge tube. Centrifuge in microfuge tube at 10,000 g for 30 sec. Pour off ...proteins and genomic DNA. Incubate tube on ice for 5 min. Centrifuge the tube for 5 min at 12,000 g. Notes:...not available. Vortex microfuge tube for 30-60 sec. Centrifuge the tube for 5 min at room temperature on... (pH 4.8). Invert the microfuge tube to mix. Optional: Place the tube either at -20 °C overnight OR -80...attached to the tube after the 70% ethanol wash. You can also pipet the supernatant out of the tube if you are...resuspended. Add 200 μL of Solution II and invert the tube carefully 5 times to mix the contents. The contents...

... mL conical tube on ice pooling the scrapings of two plates into each tube. Keep each tube on ice while...vented falcon tube for every two transformations. Each of the four 14 mL Vented Falcon Tubes should contain...weigh the empty tube beforehand! If you’ve already gone too far, weigh an identical empty tube. It will be...Reagents 200 µL electrocompetent cells (Default: 4 tubes of Endura Duos, Lucigen, 60242-1) Alternatives include...LB Agar per bioassay plate. 14 mL Vented Falcon Tubes (BD Biosciences, 352059) 3X LB Agar + Antibiotic...growth is expected to work. 50 mL Falcon Conical tubes (Fisher, 14-432-22) Reagent Preparation Prepare,...plates. Prechill Micropulser cuvettes on ice. Thaw 4 tubes of electrocompetent cells on ice for 15-20 minutes...

...PCR tube strip into a chilled 96-well freezer block. Add 20 µL of the master mix to each PCR tube. Be ...dispense to the bottom of the tube without collecting drops along the side of the tube. Cap gently - do not push...Bring the PCR tubes to the BSC used for dilutions. Without disturbing the liquid in the tube, gently uncap...uncap the PCR tubes. Add 5 µL of dilutions 6–8 to the appropriate PCR tubes. Pipette back and forth 5 times...times. Lightly cap the PCR tubes. Generate the Droplets Bring the PCR tubes to the droplet generation ...Without disturbing the liquid in the tube, gently uncap the tubes. Add 5 µL of the previously aliquoted... cartridge holder, Bio-Rad, 1863051 8-strip PCR tubes, Axygen, PCR-02-FCP-C ddPCR 96-well PCR plates, ...

...DNA 500 mL vented flask 50 mL conical tubes Microcentrifuge tubes Cell counting chamber slide 30 mL luer-lock...microcentrifuge tube. Vortex the cell suspension. Transfer 10 µL of cell suspension into the microfuge tube containing...Transfer 6 mL of BCD TFX into each of two 50 mL tubes. Cap the tubes and incubate for 1 h in the 37 °C bead bath...transfer the tubes to the biosafety cabinet. Add 180 µg of recombinant antibody plasmid DNA to one tube of 6 ...TFX. Cap the tube and vortex for 5 s to mix. Add 450 µL of 1 mg/mL PEI-MAX to the second tube of 6 mL BCD...Benchtop centrifuge compatible with 50 mL conical tubes Automated cell counter 37 °C, 5% CO 2 incubator ...HEK293 cell suspension into a clean microcentrifuge tube. Pro-Tip Cells settle quickly and need to be resuspended...

... TE solution RNase free tubes: microcentrifuge tubes, 4 mL polypropylene tubes RNase decontamination solution... sample(s).Transfer tissue/cell lysate to a 4 mL tube. Add the following sequentially to 1 mL of lysate...transfer the top, aqueous phase to a new RNAse-free tube. The mixture separates into a bottom organic layer... may have to collect twice or more from the same tube, but unlike using a p1000 tip it will give you more...more control of where you’re aiming your tip in the tube. Precipitate your sample(s). You can use either ...gel-like white pellet of total RNA in the bottom of the tube. Wash the RNA by resuspending the pellet in 0.5–...add RNase-free water or TE as soon as the entire tube is dried but while the white pellet is still visible...

...microfuge tube and vortex. 1 ng/µL : Add 450 µL of 2 ng/µL stock into 450 uL PBS in a microfuge tube and vortex...serum albumin (BSA), VWR 10273-264 50 mL conical tubes, VWR 89039-656 96-well polyester (clear) microplate...microplate seal, Thermo Scientific 5701 Microcentrifuge tubes, Neptune 3745.X Pipette tips, 1000 µL, VWR 76322...Dilute purified antigen to 20 ng/µL in a microfuge tube and vortex. Pro-Tip You may need to try a few different... of 1 ng/µL stock into 450 µL PBS in a microfuge tube and vortex. 0.25 ng/µL : Add 450 µL of 0.5 ng/µL...µL stock into 450 µL PBS in a microfuge tube and vortex. 0.125 ng/µL : Add 450 µL of 0.25 ng/µL stock ...stock into 450 µL PBS in a microfuge tube and vortex. 0 ng/µL : 450 µL PBS only. Pipette 50 µL into the first...

...mL microfuge tubes used in labs, though some larger models can hold larger conical tubes. Depending on... for simply collecting liquid to the bottom of a tube. You will likely encounter several different types...: September 2022 Equipment Centrifuge Microfuge tubes or other suitable container Personal protective ...microcentrifuge with the lid open, the rotor with microfuge tubes in place, and the control panel where you can set...example of a balanced tabletop centrifuge, with tubes evenly distributed around the rotor. How to Centrifuge...needed. This decision should be based on the size of tubes you are using and the speed at which you need to...example, spinning down samples in 1.5 mL microfuge tubes at 10,000 rpm would require a tabletop microcentrifuge...

..., VWR 89130-902 50 mL conical tubes, VWR 89039-656 Microcentrifuge tubes, VWR 89126-724 Filtering system...fractions into the tubes containing 50 µL 1 M Trizma hydrochloride pH 9.0. Cap the tubes and vortex for 5...from the collection tube each time. Place column in a new 50 mL LoBind collection tube. Slowly apply the...Benchtop centrifuge compatible with 50 mL conical tubes NanoDrop spectrophotometer 37 °C, 5% CO 2 incubator... column, Millipore Sigma UFC903024 50 mL LoBind tubes, VWR 76289-498 Before Starting Wipe down all pipettes.... re Collect the flow-through in a 50 mL conical tube and dispose after use. Carefully pour the filtered...Trizma hydrochloride pH 9.0 to 10 microcentrifuge tubes. Cap the Gravitrap columns at the bottom. Add 5 ...

...mix to add to each PCR tube. Secure the tops to the PCR tubes, gently tap each tube to bring all the liquid...times. Keep tube on ice. Add the forward and reverse primers to the thinned walled PCR tubes. Note: Do ...List Reagents for each 50µL PCR: Thin-walled PCR tube Ice Bucket 2 μL Template DNA (10 ng-500 ng) 5 μl...resource for choosing primers. Place thin-walled PCR tubes on ice Set up a 50 μL reaction (Keep all your reagents...DO NOT get added to a master mix. Place reaction tubes in PCR machine. Set annealing temperature 5°C below... we multiply each volume by 7. In a single 1.5mL tube combine the following: 10X Taq buffer with MgCl ...added, pipet the forward primer onto one side of the tube wall and the reverse primer onto the other. Add ...

...PCR tube strip into a chilled 96-well freezer block. Add 16 µL of the master mix to each PCR tube. Be ...dispense to the bottom of the tube without collecting drops along the side of the tube. Add 4 µL of the 25 ng... cartridge holder, Bio-Rad, 1863051 8-strip PCR tubes, Axygen, PCR-02-FCP-C ddPCR 96-well PCR plates, ...Polystyrene reservoirs, VWR, 89094-662 Microcentrifuge tubes, VWR, 87003-294 Primers/probe targeting RRE: forward...virus in DMEM complete: Prepare 7 microcentrifuge tubes with the appropriate amount of media volume listed... DMEM complete and transfer to a microcentrifuge tube. Centrifuge for 5 min at 100 x g . Gently aspirate...Prepare the RRE/RPP30 master mix in a microcentrifuge tube as shown below. For 8 samples prepare enough master...

...hours. c. In polypropylene microfuge tubes (do NOT use polystyrene tubes), make a cocktail for each transfection...walls of the tube before it has been diluted. Mix by swirling or gently flicking the tube. Incubate for...not onto the walls of the tube. Mix by swirling or gently flicking the tube. f. Incubate for 20-30 minutes... 7kb band and place in a sterile microcentrifuge tube. TIP: When visualizing DNA fragments to be used ...Streptomycin Invitrogen: #15140-122 Polypropylene tubes VWR: #87003-290 Note: pLKO.1 could also be packaged... μL FuGENE® + 740 μL OPTI-MEM In a polypropylene tube, add OPTI-MEM first. Pipette FuGENE® directly into...temperature. e. Add 80 μL of FuGENE® master mix to each tube from step c for a total volume of 100 μL. Pipette...

... lid and the tube of a glycerol stock before you place the sample at -80°C. Frozen tubes are hard to write...culture to 500 μL of 50% glycerol in a 2 mL screw top tube or cryovial and gently mix. Notes: Make the 50% ...solution by diluting 100% glycerol in dH20. Snap top tubes are not recommended as they can open unexpectedly...unexpectedly at -80°C. Freeze the glycerol stock tube at -80°C. The stock is now stable for years, as long as it...recover bacteria from your glycerol stock, open the tube and use a sterile loop, toothpick or pipette tip...for long periods at -80°C can lose labels stuck to tube! Reference Page Top Index...

...microcentrifuge or falcon tube. GENTLY mix by flicking the bottom of the tube with your finger a few times...shock each transformation tube by placing the bottom 1/2 to 2/3 of the tube into a 42°C water bath for...42 °C Ice bucket filled with ice Microcentrifuge tubes Sterile spreading device Reagents LB agar plate ... on the competent cells you are using). Put the tubes back on ice for 2 min. Add 250-1,000 μl LB or SOC...transformation efficiency (such as when you have a tube of plasmid DNA and just need to transform bacteria...

...Microcentrifuge tubes, Neptune 3745.X 10 cm tissue culture dish, Corning 430167 15 mL conical tubes, VWR 21008...membrane. Aliquot 500–1000 μL into sterile tubes. Store the tubes at -20 °C or -80 °C. After thawing, the... for up to 2 months. After 2 months, discard the tube and thaw a new working stock. The optimal mass DNA...PEI dropwise while gently flicking the diluted DNA tube. Incubate the mixture 12–15 min at RT. During the...transfer the harvested media to a polypropylene storage tube and store at 4 °C between harvest. Centrifuge the...

...Place the gel in a labeled microfuge tube. Using a scale, weigh the tube with the gel fragment after zeroing... with an empty tube. Alternatively, you can just subtract the weight of the empty tube from the weight...weight of the tube with the gel fragment. The weight of the gel is directly proportional to its liquid volume...

...membrane. Aliquot 500–1000 µL into sterile tubes. Store the tubes at -80 °C. After thawing the solution can...Polyethylenimine, linear MW 25,000 Da Microcentrifuge tubes, Neptune 3745.X 10 cm tissue culture dish, Corning... for up to 2 months. After 2 months, discard the tube and thaw a new working stock. Considerations Before...PEI dropwise while gently flicking the diluted DNA tube. Incubate the mixture 15–20 min at RT. Carefully...

...microcentrifuge tube, a bottle etc.). If there is already other liquid in the receiving tube, place the pipette...Containers to hold measured liquid (ex: microfuge tube, bottle, etc.) Labels for containers Reagents Liquid... avoid touching the pipette to the inside of the tube. Only the tip should be in the liquid. Pro-Tip If...pipetting the same amount of liquid into different tubes or into wells of a microplate, you may consider ...

...Place the mixed oligos in a 1.5mL microfuge tube. Place tube in 90-95°C hot block and leave for 3-5 minutes...minutes). Method #2 Place mixed oligos in a PCR tube. Place tube in a thermocycler programmed to start at 95...