We narrowed to 971 results for: lars;
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TypeBlog Post...infections, we are treating microbes with compounds similar to those they have been battling for centuries.... that will be more difficult to treat later. A similar situation can occur if you treat a bacterial infection...
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The Challenges of Cell Culture
TypeBlog Post... match their original description. The oldest, largest, and most diverse of these repositories is the ...out on low passage cells and this means you can regularly discard the cells you are working with because... -
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog Post...undergo homology-directed repair (HDR), a distinct cellular DNA repair pathway. Accomplishing this usually... iPSC-based models of neurodegenerative and neurovascular diseases. He worked on the described project... -
A Control for All Seasons
TypeBlog Post...both groups on a western blot and look for the molecular weight shifts indicative of tau phosphorylation...against a protein standard (P) to check for tau molecular weight shifts. A negative control is a sample... -
Transferable Skills Guide: Teamwork
TypeBlog Post... looking at a process-oriented team serving a particular function. Project team A Project Team is assembled...an excuse. Every psych test I’ve ever taken has declared me an introvert, and I identify as one. But some... -
DNA Quantification
TypeProtocol...Information During several different stages of molecular cloning, it is important to get a quick and accurate... a liquid to determine the concentration of a particular substance in that liquid. Molecules absorb different... -
Protocol - How to Design Primers
TypeProtocol...also use a nucleotide-based molecular insert (inosine) instead of a regular nucleotide for broader pairing... -
Protocol - How to Streak a Plate
TypeProtocol... plate, do NOT dig into the agar. Another very popular technique is to draw in discontinuous lines. Start...30 °C instead of 37 °C. This is often true for large unstable plasmids, which sometimes recombine at ... -
How to Deposit Your Plasmids with Addgene
TypeBlog Post[email protected]. These deposits are considered large deposits and are exceptions to our standard workflow...your plasmid is designed to allow you to produce large amounts of your insert in E. coli, then this is ... -
Plasmid Modification by Annealed Oligo Cloning (with Protocols)
TypeProtocol... pH7.5-8.0, 50mM NaCL, 1mM EDTA) and mixed in equimolar concentrations. We recommend mixing 2μg each ...μl). Mix the annealed oligos with cut vector in molar ratios (vector:insert) between 4:3 and 1:6 in a ...